Scientia Sinica Vitae, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 29, 2024
Heliyon, Journal Year: 2024, Volume and Issue: 10(17), P. e37192 - e37192
Published: Aug. 30, 2024
In immunology, the role of macrophages extends far beyond their traditional classification as mere phagocytes; they emerge pivotal architects immune response, with function being significantly influenced by multidimensional environmental stimuli. This review investigates nuanced mechanisms which diverse external signals ranging from chemical cues to physical stress orchestrate macrophage polarization, a process that is crucial for modulation responses. By transitioning between pro-inflammatory (M1) and anti-inflammatory (M2) states, exhibit remarkable plasticity, enabling them adapt influence surroundings effectively. The exploration polarization provides compelling narrative on how these cells can be manipulated foster an environment conducive tissue repair regeneration. Highlighting cutting-edge research, this presents innovative strategies leverage dynamic interplay environment, proposing novel therapeutic avenues harness potential in regenerative medicine. Moreover, critically evaluates current challenges future prospects translating macrophage-centered laboratory clinical applications.
Language: Английский
Citations
4
Molecular Aspects of Medicine, Journal Year: 2025, Volume and Issue: 102, P. 101338 - 101338
Published: Feb. 19, 2025
Language: Английский
Citations
0
Nutrients, Journal Year: 2025, Volume and Issue: 17(6), P. 964 - 964
Published: March 10, 2025
Background/Objectives: Our lab has previously reported that Grifola frondosa (maitake mushroom) GF5000 antidiabetic potential owing to its ability improve insulin resistance. This study aimed gain insight into the system-level hypoglycemic mechanisms of using transcriptomics, proteomics, and network pharmacology. provides new insights GF5000, identifying key molecular targets involved in mitigating resistance T2DM. Methods: Liver protein gene expression normal control (NC), diabetic (DC), GF5000-treated (GF5000) rats were analyzed via iTRAQ RNA-seq. The relationships between differentially expressed genes (DEGs), proteins (DEPs), type 2 diabetes (T2DM) disease studied Metascape Cytoscape GeneMANIA plug-in. Results: One hundred fifty-two DEGs sixty-two DEPs identified; twenty DEGs/DEPs exhibited same trend mRNA levels when comparing vs. DC groups. analysis revealed T2DM included four DEGs—Gck, Scd, Abcb4, Cyp3a9—and two DEPs—glucokinase acetyl-CoA carboxylase 2. A Cytoscape–GeneMANIA thirteen related showed Apoa1/Apolipoprotein A-I, Gckr/glucokinase regulatory protein, Gck/glucokinase had highest connectivity centrality topological network. qPCR results confirmed increased GCK GCK-knockdown HepG2 cells. Conclusions: These provide theoretical evidence for use as a active nutritional ingredient prevention treatment findings suggest multiple pathways implicated T2DM, offering multi-faceted approach management prevention.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: March 25, 2025
Right atrial (RA) dysfunction is an emerging risk factor for poor outcomes in pulmonary arterial hypertension, however the mechanisms underlying compromised RA function are understudied. Multi-omic analyses defined cellular and molecular mediators associated with artery banded (PAB) swine. 4-week-old castrated male Yorkshire pigs were subjected to PAB aged six weeks induce right heart failure. Cardiac MRI evaluated size function. snRNAseq cell-specific alterations tissue. Mitochondrial proteomics metabolomics examined metabolic samples. Inducible pluripotent stem cell-derived cardiomyocytes (iPSC-ACM) treated tunicamycin endoplasmic reticulum (ER) stress mitochondrial structure probed. induced dilation/dysfunction cardiomyocyte hypertrophy. demonstrated altered composition of by increased inflammatory macrophages alteration subpopulations. exhibited ER enzyme dysregulation. RAs, but not ventricles, had downregulation branched chain amino acid degrading enzymes. Metabolomics profiling revealed BCAA fatty metabolism impaired dysfunctional RA. Tunicamycin-induced disrupted structure/function iPSC-ACMs. evaluations demonstrate characterized derangements due dysregulation accumulation pro-inflammatory macrophages.
Language: Английский
Citations
0
Advanced Science, Journal Year: 2025, Volume and Issue: unknown
Published: April 8, 2025
Abstract Baicalin has been widely used for its anti‐inflammatory pharmacological properties, yet effects on bacterial intestinal inflammation and the mechanisms remain unclear. This study revealed that baicalin alleviates through regulating macrophage polarization increasing Lactobacillus amylovorus abundance in colon. Specifically, transcriptomic analysis showed restored Escherichia coli ‐induced genes expression changes including T helper cell 17 differentiation‐related genes, related TLR/IRF/STAT signaling pathway. Subsequent microbial non‐targeted metabolomic these may be to enhancement of upregulation metabolites chrysin, lactic acid, indoles. Furthermore, whole‐genome sequencing provided insights into functional potential metabolic annotations. supplementation mice similarly inhibited M1 TLR4/IRF/STAT Additionally, baicalin, , or chrysin alone could regulate polarization, highlighting their independent potential. Notably, this pathway synthesis chrysin. These findings provide new therapeutic preventing treating inflammation, offering key targets future interventions.
Language: Английский
Citations
0
Algal Research, Journal Year: 2024, Volume and Issue: 84, P. 103742 - 103742
Published: Oct. 9, 2024
Language: Английский
Citations
1
Published: Jan. 1, 2024
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0
Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 18, 2024
Building upon previous structure–activity relationships about the fungicidal amide and hydrazide lead structures, 24 novel amide-hydrazide compounds were designed synthesized with L-isoleucine as initial skeleton to explore impact of substituents in bridge on activity. Among these compounds, A5 exhibited excellent broad spectrum inhibitory activity, along satisfactory vivo protective efficiency against R. solani at concentrations 200 50 μg·mL–1. Scanning electron microscopy (SEM) transmission (TEM) observations revealed that compound induced significant morphological changes mycelium coupled vacuole rupture cytoplasmic inhomogeneity cellular structures. Transcriptomic metabolomic analyses indicated that, following treatment, differentially expressed genes metabolites significantly enriched carbohydrate metabolism-related pathways well lipid metabolism-associated pathways, including glycerophospholipid metabolism, steroid biosynthesis, arachidonic acid sphingolipid metabolism. Additionally, demonstrated low toxicity zebrafish, survival rates 100% 60% 1 10 μg·mL–1, respectively, over a period 7 days. The above results provide theoretical guidance for development green candidates.
Language: Английский
Citations
0
Scientia Sinica Vitae, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 29, 2024
Citations
0