Unfolded proteins in the mitochondria activate HRI and shut-down of mitochondrial protein translation DOI Creative Commons

Yongshu Wu,

Yang Yang,

Xiaodong Qin

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 21, 2024

Abstract Mitochondrial unfolded protein response (UPR mt ) is triggered through eIF2α phosphorylation in mammal. However, the mechanisms of UPR activation and influence on mitochondrial translation remain unclear. In this study, we confirmed that was a rapid specific stress with pharmacological induction, along expression phosphorylation, ATF4, CHOP. Meanwhile, up-regulation some chaperones, cytochrome P450 enzymes, DDIT4 determined by RNA-Seq ribosome profiling, essential for expressing ATF4 CHOP, then traffics into nucleus initiates CHOP expression. addition, generation ROS morphology unchanged under GTPP induced . Furthermore, unraveled mechanism HRI kinase mediates proteins CRISPR-Cas9 technology recruitment interaction other proteins. number imports were inhibited accumulation protein. These findings provide molecular impact cellular translation, which will offer novel insights functional research , including its implications human diseases pathobiology.

Language: Английский

SIRT2 Inhibition Enhances Mitochondrial Apoptosis in Brucella-Infected Bovine Placental Trophoblast Cells DOI Creative Commons
M. Zhang, Lin Qi,

Junmei Li

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

Abstract Brucella being a successful pathogen, employs plethora of immune evasion mechanisms. This contributes to pathogenesis, persistence and also limits the efficacy available treatment. Increasing understanding host-pathogen interactions suggests that integrating host-directed strategies with existing anti-Brucella treatments could lead more effective bacterial clearance reduction in drug-resistant strains. SIRT2 is nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase found mammals. It can deacetylate various transcription factors regulatory proteins, playing crucial role pathogen infection-induced apoptosis. In this study, we investigate Brucella-induced cell apoptosis using bovine placental trophoblast cells. Our results indicate B. abortus A19 infection upregulates protein expression significantly induces mitochondrial these Furthermore, Inhibition exacerbates A19-induced markedly inhibits intracellular survival. These prove pathogenesis mechanism action.

Language: Английский

Citations

1
Inter- and intracellular mitochondrial communication: signaling hubs in aging and age-related diseases DOI Creative Commons
Meng Zhang, Wei Jin, Chang He

et al.

Cellular & Molecular Biology Letters, Journal Year: 2024, Volume and Issue: 29(1)

Published: Dec. 18, 2024

Abstract Mitochondria are versatile and complex organelles that can continuously communicate interact with the cellular milieu. Deregulated communication between mitochondria host cells/organelles has significant consequences is an underlying factor of many pathophysiological conditions, including process aging. During aging, lose function, mitocellular pathways break down; mitochondrial dysfunction interacts dyscommunication, forming a vicious circle. Therefore, strategies to protect function promote effective increase healthy lifespan longevity, which might be new treatment paradigm for age-related disorders. In this review, we comprehensively discuss signal transduction mechanisms inter- intracellular communication, as well interactions hallmarks This review emphasizes indispensable position in aging organisms, crucial signaling hubs. addition, also specifically focus on status mitochondria-targeted interventions provide potential therapeutic targets diseases. Graphical

Language: Английский

Citations

0
Control of Mitochondrial Quality: A Promising Target for Diabetic Kidney Disease Treatment DOI Creative Commons
Q Li, Jin Shang, Reiko Inagi

et al.

Kidney International Reports, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

0
Unfolded proteins in the mitochondria activate HRI and shut-down of mitochondrial protein translation DOI Creative Commons

Yongshu Wu,

Yang Yang,

Xiaodong Qin

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 21, 2024

Abstract Mitochondrial unfolded protein response (UPR mt ) is triggered through eIF2α phosphorylation in mammal. However, the mechanisms of UPR activation and influence on mitochondrial translation remain unclear. In this study, we confirmed that was a rapid specific stress with pharmacological induction, along expression phosphorylation, ATF4, CHOP. Meanwhile, up-regulation some chaperones, cytochrome P450 enzymes, DDIT4 determined by RNA-Seq ribosome profiling, essential for expressing ATF4 CHOP, then traffics into nucleus initiates CHOP expression. addition, generation ROS morphology unchanged under GTPP induced . Furthermore, unraveled mechanism HRI kinase mediates proteins CRISPR-Cas9 technology recruitment interaction other proteins. number imports were inhibited accumulation protein. These findings provide molecular impact cellular translation, which will offer novel insights functional research , including its implications human diseases pathobiology.

Language: Английский

Citations

0