Molecular Cytogenetics in Domestic Bovids: A Review
Animals,
Journal Year:
2023,
Volume and Issue:
13(5), P. 944 - 944
Published: March 6, 2023
The
discovery
of
the
Robertsonian
translocation
(rob)
involving
cattle
chromosomes
1
and
29
demonstration
its
deleterious
effects
on
fertility
focused
interest
many
scientific
groups
using
chromosome
banding
techniques
to
reveal
abnormalities
verify
their
in
domestic
animals.
At
same
time,
comparative
studies
among
various
species
or
wild
animals
were
found
useful
for
delineating
evolution
species.
advent
molecular
cytogenetics,
particularly
use
fluorescence
situ
hybridization
(FISH),
has
allowed
a
deeper
investigation
through:
(a)
physical
mapping
specific
DNA
sequences
regions;
(b)
markers
identification
regions
involved
abnormalities,
especially
when
poor
patterns
are
produced;
(c)
better
anchoring
radiation
hybrid
genetic
maps
(d)
comparisons
related
unrelated
by
FISH
and/or
Zoo-FISH
techniques;
(e)
study
meiotic
segregation,
sperm-FISH,
some
abnormalities;
(f)
conserved
lost
(g)
informatic
genomic
reconstructions,
addition
CGH
arrays,
predict
species;
(h)
stability
PCR
applications.
This
review
summarizes
most
important
applications
cytogenetics
bovids,
with
an
emphasis
Language: Английский
Expression of miR-138 in cryopreserved bovine sperm is related to their fertility potential
Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: Sept. 20, 2023
MicroRNAs
(miRNAs)
are
small,
single-stranded,
non-coding
RNA
molecules
of
22-24
nucleotides
that
regulate
gene
expression.
In
the
last
decade,
miRNAs
have
been
described
in
sperm
several
mammals,
including
cattle.
It
is
known
can
act
as
key
regulators
early
embryogenesis
mice
and
humans;
however,
little
about
content,
expression,
function
sperm-borne
bovine
embryo.
this
study,
total
was
isolated
from
29
cryopreserved
samples
(each
coming
a
separate
bull)
using
RNeasy
kit
treatment
with
DNase
I.
concentration
purity
were
determined
through
an
Epoch
spectrophotometer
Agilent
Bioanalyzer.
The
expression
10
candidate
(bta-miR-10a,
bta-miR-10b,
bta-miR-138,
bta-miR-146b,
bta-miR-19b,
bta-miR-26a,
bta-miR-34a,
bta-miR-449a,
bta-miR-495
bta-miR-7),
previously
identified
testis
and/or
epididymis,
evaluated
RT-qPCR.
cel-miR-39-3p
used
spike-in
exogenous
control.
Nonparametric
Mann-Whitney
tests
run
to
evaluate
which
differentially
expressed
between
bulls
high
fertility
[HF;
non-return
rates
(NRR)
ranging
39.5
43.5]
those
subfertility
(SF;
NRR
33.3
39.3).
Several
functionality
parameters
(e.g.,
viability,
membrane
stability
or
oxygen
consumption,
among
others)
measured
by
multiplexing
flow
cytometry
sensing
technologies.
(260/280
nm
ratio)
(mean
±
SD)
99.3
84.6
ng/µL
1.97
0.72,
respectively.
Bioanalyzer
results
confirmed
lack
somatic
cells.
terms
presence
absence
miRNAs,
after
applying
Livak
method,
8
out
(bta-miR-10b,
-138,
-146b,
-19b,
-26a,
-449a,
-495,
-7)
consistently
detected
sperm,
whereas
other
two
-34a)
absent.
Interestingly,
relative
one
miRNA
(bta-miR-138)
significantly
lower
SF
than
HF
group
(P
=
0.038).
addition
being
associated
potential,
found
be
negatively
correlated
consumption.
three
(bta-miR-19b,
bta-miR-26a
bta-miR-7)
also
variables.
conclusion,
although
functional
validation
studies
required
confirm
these
results,
study
suggests
bta-miR-138
involved
fertilization
events
beyond,
supports
its
use
biomarker
Language: Английский
Telomere Dynamics in Livestock
Biology,
Journal Year:
2023,
Volume and Issue:
12(11), P. 1389 - 1389
Published: Oct. 31, 2023
Telomeres
are
repeated
sequences
of
nucleotides
at
the
end
chromosomes.
They
deteriorate
across
mitotic
divisions
a
cell.
In
Homo
sapiens
this
process
lifetime
reduction
has
been
shown
to
correspond
with
aspects
organismal
aging
and
exposure
stress
or
other
insults.
The
early
impetus
characterize
telomere
dynamics
in
livestock
related
concern
that
aged
donor
DNA
would
result
earlier
cell
senescence
overall
cloned
animals.
Telomere
length
investigations
dairy
cows
included
breed
effects,
estimates
additive
genetic
control
(heritability
0.12
0.46),
effects
external
stressors
on
degradation
animal
life.
Evaluation
telomeres
respect
also
conducted
pigs
horses,
there
fewer
reports
biology
beef
cattle,
sheep,
goats.
There
were
minimal
associations
productivity
measures.
Most,
but
not
all,
work
documented
an
inverse
relationship
between
peripheral
blood
age;
is,
longer
was
associated
younger
age.
Because
longevity
affects
profitability,
role
tissue-specific
attrition
may
present
alternative
improvement
strategies
for
while
providing
translational
biomedical
knowledge.
Language: Английский
Influence of breed and environment on leukocyte telomere length in cattle
Vavilov Journal of Genetics and Breeding,
Journal Year:
2024,
Volume and Issue:
28(2), P. 190 - 197
Published: April 11, 2024
High
milk
yield
is
associated
with
reduced
longevity
in
high-producing
dairy
cattle
breeds.
Pre-term
culling
leads
to
high
replacement
heifer
demand
and
economic
losses
for
the
industry.
Selection
this
trait
limited
because
of
low
heritability
difficulties
phenotype
measurement.
Telomeres
are
elements
found
at
ends
chromosomes,
consisting
repetitive
DNA
sequences,
several
thousand
base
pairs
length,
coupled
nucleoprotein
complexes.
Eventually,
humans
most
other
animals,
telomere
length
reduces
age.
When
telomeric
truncated
a
critical
cell
ageing,
cycle
arrest,
apoptosis
induced.
As
result,
can
be
considered
as
predictor
health
risks
an
individual’s
lifespan.
The
leukocyte
may
used
proxy
productive
lifespan
improve
selection.
Our
objectives
were
assess
effects
breed
group
(dairy
vs.
beef)
on
estimate
effect
cold
climate
Kalmyk
populations
from
South
(Rostov
Oblast)
Far
North
(Republic
Sakha)
regions
Russia.
lengths
estimated
computationally
whole-genome
resequencing
data.
We
leveraged
data
sex,
age
239
animals
17
factor
had
significant
across
our
sample.
There
was
no
difference
between
beef
groups.
population
animals.
In
conclusion,
we
that
breed,
but
not
beef),
significantly
cattle.
Residence
colder
climates
longer
Language: Английский
Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021-2023
Czech Journal of Animal Science,
Journal Year:
2023,
Volume and Issue:
68(12), P. 469 - 485
Published: Dec. 20, 2023
Flow
cytometry
(FC)
is
a
very
powerful
method
for
the
assessment
of
spermatozoa
quality
in
multiple
livestock
species,
including
bulls.
Nowadays,
many
flow
cytometric
tests
are
available
detection
bull
sperm.
These
based
on
fluorochromes
(fluorescent
tags
or
dyes)
and
use
correct
concentrations
an
essential
aspect
successful
optimization
experimental
methodology.
Modern
cytometers
much
more
affordable,
such
that
spermatologists
now
able
to
FC
methodology
(and
even
image
cytometry)
their
studies.
The
present
review
aims
give
summary
current
methodologies
used
assess
sperm
by
cytometry.
Namely,
we
here
studies
related
evaluation
published
last
three
years
(from
2021
2023).
Importantly,
review,
concentration
unified
presented
using
comparable
molarity
units.
Furthermore,
rationale
each
particular
fluorescent
tests,
citation
corresponding
original
methodology,
given.
This
will
help
shorten
time
needed
quality.
Language: Английский