Effect of the GnRH stimulation test on Canine Prostatic‐Specific Esterase (CPSE) DOI
Lluis Ferré‐Dolcet, Maria Carmela Pisu,

Simona Attard

et al.

Reproduction in Domestic Animals, Journal Year: 2023, Volume and Issue: 58(8), P. 1125 - 1131

Published: June 20, 2023

The gonadotropin-releasing hormone (GnRH) stimulation test is used to investigate testicular production of testosterone (T) when performing a breeding soundness examination. In male dogs with fertility problems, the prostate should also be investigated as prostatic conditions may frequently lower semen quality. Serum concentrations canine prostatic-specific esterase (CPSE) increase in benign hyperplasia (BPH). When examination dog, GnRH administration done at beginning process and then both T CPSE are assayed on same serum sample collected 1 h following injection. aim this study was assess whether or not alter healthy prostate. Twenty-eight client-owned intact adult were included study. Following 7-day sexual rest all underwent clinical an ultrasonographic gland. Prostatic size parenchyma every tested dog evaluated by ultrasonography conditions. Two different protocols used, A = gonadorelin 50μg/dog SC (n 15) B buserelin 0.12 μg/kg IV 13). measured before after laser-induced fluorescence analysis. Buserelin equally effective causing significant post sample. considering 28 together, did change either compound; however, 4/28 cases, value markedly increased values compatible diagnosis BPH. There no difference action concentrations. secretion approximately 15% treated gonadorelin. Therefore, whenever diagnostic testing dogs, post-GnRH

Language: Английский

Multiparametric ultrasound (MPUS) evaluation of the testes of normozoospermic dogs – a pilot study DOI Creative Commons

Luiz Paulo Nogueira Aires,

B Gasser,

Priscila Silva

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 28, 2025

Ultrasound is an important tool in small animal andrology for assessing the integrity of testes. This study explores ultrasound's role canine testes and understanding correlation sonographic parameters to sperm quality. It investigates B-mode Doppler ultrasound techniques alongside advanced methods like Shear-wave Elastography (SWE) Contrast-enhanced (CEUS). The aim was standardize a multiparametric (MPUS) evaluation protocol using these normozoospermic dogs. Eight healthy male dogs were assessed. assessed testicular morphology, while evaluated artery waveform morphology velocimetric parameters. SWE measured stiffness, CEUS perfusion. Seminal also analyzed. Results revealed normal findings some correlations between demonstrated consistent stiffness regardless depth evaluation, with head epididymis ejaculate volume. identified blood flow differences perfusion correlated features. comprehensive assessment provides valuable insights into reproductive health, providing basis further studies on abnormal quality different fertility statuses.

Language: Английский

Citations

0
Defining an Optimal Range of Centrifugation Parameters for Canine Semen Processing DOI Creative Commons

Nicole Sugai,

Stephen R. Werre,

Julie T. Cecere

et al.

Animals, Journal Year: 2023, Volume and Issue: 13(8), P. 1421 - 1421

Published: April 21, 2023

Our objective was to determine a clinically relevant range of centrifugation parameters for processing canine semen. We hypothesized that higher gravitational (g) force and longer time would result in improved spermatozoa recovery rate (RR) but poorer semen quality. Cooled storage under standard shipping conditions used as stressor evaluate long-term treatment effects. Individual ejaculates collected from 14 healthy dogs were split into six groups (400 g, 720 900 g 5 or 10 min). Sperm RR (%) calculated post-centrifugation, plasma membrane integrity (%, Nucleocounter® SP-100™), total progressive motility subjective computer-assisted sperm analysis), morphology eosin-nigrosin staining) assessed on initial raw (T0), post-centrifugation (T1), 24 h (T2) 48 (T3) after cooling. losses minimal, RRs similar across (median >98%, p ≥ 0.062). Spermatozoa not different between at any point (p 0.38) declined significantly during cooling (T1 vs. T2/T3, ≤ 0.001). Similarly, did differ treatments all T1 T3 0.02). In conclusion, our study showed within 400 g-900 5-10 min is appropriate

Language: Английский

Citations

5
Canine sperm motility is associated with telomere shortening and changes in expression of shelterin genes DOI Creative Commons
Hossein Hassanpour, Pezhman Mirshokraei,

Marzieh Salehpour

et al.

BMC Veterinary Research, Journal Year: 2023, Volume and Issue: 19(1)

Published: Nov. 10, 2023

Abstract Background Motion quality is a critical property for essential functions. Several endogenous and exogenous factors are involved in sperm motility. Here, we measured the relative telomere length evaluated gene expression of its binding-proteins, shelterin complex ( TRF1, TRF2, RAP1, POT1, TIN2 , TPP1 ) dogs using quantitative real-time PCR. We compared them between two subpopulations with poor good motion qualities (separated by swim-up method). Telomere shortening alterations result from ROS, genotoxic insults, genetic predisposition. Results Sperm kinematic parameters were then telomeric index each parameter was calculated. Telomeric linearity, VSL, VCL, STR, BCF, ALH significantly higher sperms than quality. demonstrated that associated shorter telomere, POT1 genes, lower RAP1 dog sperm. The levels TRF1 remained consistent despite variations length. Conclusion Data provided evidence there considerable changes many components TIN2, spermatozoa Possibly, defects Our data suggests new approach semen assessment etiologic investigations subfertility or infertility male animals.

Language: Английский

Citations

2
Canine semen processing for chilled shipment DOI Creative Commons

Julie T. Cecere,

Nicole Sugai

Clinical Theriogenology, Journal Year: 2023, Volume and Issue: 15

Published: Dec. 29, 2023

Advanced reproductive techniques for dogs have become commonplace in general practice during last decade. Most procedures performed these practices are based on what is known semen processing and shipping the equine industry. This presentation reviews current literature of canine attempts to standardize maximize their successful use clinical settings.

Language: Английский

Citations

1
Effect of the GnRH stimulation test on Canine Prostatic‐Specific Esterase (CPSE) DOI
Lluis Ferré‐Dolcet, Maria Carmela Pisu,

Simona Attard

et al.

Reproduction in Domestic Animals, Journal Year: 2023, Volume and Issue: 58(8), P. 1125 - 1131

Published: June 20, 2023

The gonadotropin-releasing hormone (GnRH) stimulation test is used to investigate testicular production of testosterone (T) when performing a breeding soundness examination. In male dogs with fertility problems, the prostate should also be investigated as prostatic conditions may frequently lower semen quality. Serum concentrations canine prostatic-specific esterase (CPSE) increase in benign hyperplasia (BPH). When examination dog, GnRH administration done at beginning process and then both T CPSE are assayed on same serum sample collected 1 h following injection. aim this study was assess whether or not alter healthy prostate. Twenty-eight client-owned intact adult were included study. Following 7-day sexual rest all underwent clinical an ultrasonographic gland. Prostatic size parenchyma every tested dog evaluated by ultrasonography conditions. Two different protocols used, A = gonadorelin 50μg/dog SC (n 15) B buserelin 0.12 μg/kg IV 13). measured before after laser-induced fluorescence analysis. Buserelin equally effective causing significant post sample. considering 28 together, did change either compound; however, 4/28 cases, value markedly increased values compatible diagnosis BPH. There no difference action concentrations. secretion approximately 15% treated gonadorelin. Therefore, whenever diagnostic testing dogs, post-GnRH

Language: Английский

Citations

0