Melatonin Acts Through Different Mechanisms to Control Oxidative Stress and Primordial Follicle Activation and Survival During in Vitro Culture of Bovine Ovarian Tissue DOI
Bianca R. Silva, Francisco C. Costa, Miguel F. De Lima Neto

et al.

Published: Jan. 1, 2023

This study aims to evaluate the effects of melatonin on primordial follicle activation and survival, stromal cell density collagen distribution in extracellular matrix (ECM). The involvement receptors mTORC1 pathway these processes were also investigated. To this end, ovarian fragments culture for six days α‐MEM + alone or supplemented with 1000 pM melatonin, both luzindole (inhibitor receptors), 0.16 µg/ml rapamycin (mTORC1 inhibitor). At end period, tissues processed classical histology follicles classified as normal degenerated, well growing follicles. ECM evaluated. Samples destined measure levels thiol mRNA CAT, SOD, GPX1 PRDX1, activity antioxidant enzymes GPX1. results showed that cultured had significantly higher percentage morphologically than those control medium (α‐MEM+). presence either rapamycin, however, did not block positive survival (P>0.05). reduced increased development follicles, but blocked by (P<0.05). Melatonin, influence number cells. In contrast, percentages tissues, rapamycin. Tissues CAT lower GPx when compared medium. conclusion, promotes activation, increases fiber vitro bovine tissue through its membrane-coupled mTORC1. On other hand, increase acting pathways, since it can pass membranes directly regulate oxidative stress.

Language: Английский

Synthesis and application of gold nanoparticles as antioxidants DOI Creative Commons
Babay Asih Suliasih, Setia Budi, Haliza Katas

et al.

Pharmacia, Journal Year: 2024, Volume and Issue: 71, P. 1 - 19

Published: May 23, 2024

Non-communicable diseases (NCDs) and premature aging, caused by free radicals, have spurred a demand for extensive research into finding effective antioxidants. Currently, there is an abundance of both natural synthetic antioxidants, including metal nanoparticles with high antioxidant activity. Among these, gold (AuNPs) stand out as favoured antioxidants because their minimal toxicity, simple synthesis, detectability. The properties AuNPs enhance its wide-ranging potential use in healthcare applications anti-aging, anti-inflammatory, wound healing agents, well treatment various diseases. This review highlights recent progress the synthesis method assessing capacity delves mechanism action explores health applications. In conclusion, considering physicochemical biological properties, along benefits challenges future development, are deemed promising suitable clinical

Language: Английский

Citations

12
Ascorbic acid and resveratrol improve the structural integrity of the extracellular matrix and enhance follicular survival in cultured bovine ovarian tissue DOI
Francisco C. Costa, J.R.V. Silva,

Francisco F. Caetano Filho

et al.

Theriogenology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Melatonin acts through different mechanisms to control oxidative stress and primordial follicle activation and survival during in vitro culture of bovine ovarian tissue DOI
Bianca R. Silva, Francisco C. Costa, Miguel F. De Lima Neto

et al.

Domestic Animal Endocrinology, Journal Year: 2023, Volume and Issue: 86, P. 106824 - 106824

Published: Nov. 8, 2023

Language: Английский

Citations

8
α-Pinene Improves Follicle Morphology and Increases the Expression of mRNA for Nuclear Factor Erythroid 2-Related Factor 2 and Peroxiredoxin 6 in Bovine Ovarian Tissues Cultured In Vitro DOI Creative Commons
Venância Antônia Nunes Azevedo,

Ernando Igo Teixeira de Assis,

Anderson Weiny Barbalho Silva

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(10), P. 1443 - 1443

Published: May 12, 2024

Oxidative stress during in vitro of ovarian tissues has adverse effects on follicle survival. α-pinene is a monoterpenoid molecule with antioxidant activity that great potential to maintain cell survival vitro. This study investigated the effect (1.25, 2.5, 5.0, 10.0, or 20.0 μg/mL) primordial growth and morphology, as well stromal cells collagen fibers bovine slices cultured for six days. The transcripts catalase (CAT), superoxide dismutase (SOD), peroxiredoxin 6 (PRDX6), glutathione peroxidase (GPX1), nuclear factor erythroid 2-related 2 (NRF2) was by real-time PCR. were processed histological analysis evaluate follicular growth, density, fibers. results showed 10.0 µg/mL increased percentages normal follicles but did not influence growth. (10.0 µg/mL) kept density levels tissue like uncultured tissues. Ovarian control medium had reduced expression mRNA NRF2, SOD, CAT, GPX1, PRDX6, NRF2 PRDX6. In conclusion, improves survival, preserves levels, increases PRDX6 after culture tissue.

Language: Английский

Citations

2
Alpha lipoic acid controls degeneration and ensures follicular development in ovine ovarian tissue cultured in vitro DOI
Lucy Vanessa Sulca Ñaupas,

F.D.R. Gomes,

Anna Clara Accioly Ferreira

et al.

Theriogenology, Journal Year: 2024, Volume and Issue: 225, P. 55 - 66

Published: May 19, 2024

Language: Английский

Citations

1
Punicalagin increases follicular activation, development and activity of superoxide dismutase 1, catalase, and glutathione peroxidase 1 in cultured bovine ovarian tissues DOI

Vitória Santos Bezerra,

Francisco C. Costa,

Francisco F. Caetano Filho

et al.

Reproduction Fertility and Development, Journal Year: 2024, Volume and Issue: 36(13)

Published: Aug. 11, 2024

Context The overproduction of reactive oxygen species (ROS) during in vitro culture ovarian tissues impairs follicular development and survival. Aims To evaluate the effects punicalagin on survival primordial follicles, stromal cell collagen fibres, as well levels mRNA for nuclear factor erythroid 2-related 2 (NRF2 ), superoxide dismutase 1 (SOD1 catalase (CAT glutathione peroxidase (GPX1 ) perirredoxin 6 (PRDX6 activity antioxidant enzymes cultured bovine tissues. Methods Bovine cortical were 6days α-MEM+ alone or with 1.0, 10.0, 100.0μM at 38.5°C 5% CO2 . Follicle morphology growth, density, fibres evaluated by classical histology, while expression was real-time PCR. analysed Bradford method. Key results Punicalagin improved follicle development, reduced SOD1 CAT , but did not influence cells fibres. (10.0μM) increased thiol SOD1, GPX1 enzymes. Conclusions promotes activates Implications improves

Language: Английский

Citations

1
Efeito da rutina sobre a maturação in vitro de oócitos de ovelhas obtidos de folículos pré-antrais cultivados in vitro DOI Creative Commons
Maria Lilian Gomes Loiola Torres, Kíscyla Oliveira de Andrade, Regina Lúcia dos Santos Silva

et al.

Semina Ciências Agrárias, Journal Year: 2024, Volume and Issue: 45(1), P. 207 - 226

Published: Feb. 22, 2024

Os objetivos deste estudo foram verificar o efeito da rutina sobre a maturação in vitro (MIV) de oócitos provenientes folículos secundários ovelhas cultivados e analisar possível envolvimento via mTOR na MIV, sob influência rutina. por 18 dias em meio α-Mínimo Essencial (α-MEM) suplementado com albumina sérica bovina (BSA), insulina, glutamina, hipoxantina, transferrina, selênio, ácido ascórbico leptina (meio controle: α-MEM+). Em seguida, os avaliados quanto à morfologia, formação do antro diâmetro folicular taxa totalmente crescidos (≥110 µm). Oócitos submetidos MIV cultivo tecidos 199 (TCM199) soro fetal bovino (FBS), hormônio luteinizante (LH), folículo estimulante recombinante (rFSH) controle MIV) ou neste 0,1, 1 10 µg.mL-1 Ao final atividade mitocondrial, concentração espécies reativas oxigênio (ERO) glutationa (GSH), porcentagem retomada meiose, fragmentação DNA mTOR. Após vitro, 77,5% dos estavam normais 77,7% tornaram-se antrais, 380,41 µm diâmetro. Além disso, 70% que cresceram atingiram ≥110 MIV. A aumentou significativamente retomaram meiose (47,27%) comparação ao (30,43%). Houve um aumento significativo nas concentrações ROS GSH maturados 0.1 outros tratamentos (p < 0,05). TCM199+ (p<0,05) percentual (30%) comparado tratamento (0%). ambos presença ausência rapamicina apresentaram semelhante meiótica (61,76% para TCM199 + 70,73% rapamicina) (p>0,05). conclusão, reduziu danos DNA.

Citations

0
In Vitro Culture of Bovine Ovarian Cortex Induces Structural Remodeling of Ovarian Extracellular Matrix and Reduces Stromal Cells and Enzymatic Antioxidant Protection DOI
Francisco C. Costa, Bianca R. Silva,

Francisco F. Caetano Filho

et al.

Published: Jan. 1, 2024

This study aimed to investigate the changes in extracellular matrix and number of stromal cells around primordial primary follicles culture bovine ovarian tissues. Furthermore, follicular oocyte diameters, granulosa cell survival, activity antioxidant enzymes were assessed. To this end, fragments cultured α-minimum essential medium (α-MEM+) for 6 days. Before (uncultured control) after culturing, fixed, processed histological analysis stained with hematoxylin eosin assess survival measure diameters. analyze collagen fibers glycosaminoglycans, sections picrosirius red or Alcian blue. Uncultured tissue samples also used mRNA expression superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), peroxiredoxin (PRDX). The thiol levels CAT, SOD, GPX investigated. results demonstrated that, 6-day tissues, GAGs network exhibited a great degree disorganization, deposition these components surrounding was significantly reduced when compared uncultured (P<0.0001). (P<0.05) (P<0.0001) In addition, number, as well diameters both categories Regarding protection, GPX, PRDX downregulated Similarly, CAT reduced. conclusion, vitro affects abundance organization glycosaminoglycans network, reduces stroma follicles. resulted lower proportion health cells, protection.

Language: Английский

Citations

0
Melatonin Acts Through Different Mechanisms to Control Oxidative Stress and Primordial Follicle Activation and Survival During in Vitro Culture of Bovine Ovarian Tissue DOI
Bianca R. Silva, Francisco C. Costa, Miguel F. De Lima Neto

et al.

Published: Jan. 1, 2023

This study aims to evaluate the effects of melatonin on primordial follicle activation and survival, stromal cell density collagen distribution in extracellular matrix (ECM). The involvement receptors mTORC1 pathway these processes were also investigated. To this end, ovarian fragments culture for six days α‐MEM + alone or supplemented with 1000 pM melatonin, both luzindole (inhibitor receptors), 0.16 µg/ml rapamycin (mTORC1 inhibitor). At end period, tissues processed classical histology follicles classified as normal degenerated, well growing follicles. ECM evaluated. Samples destined measure levels thiol mRNA CAT, SOD, GPX1 PRDX1, activity antioxidant enzymes GPX1. results showed that cultured had significantly higher percentage morphologically than those control medium (α‐MEM+). presence either rapamycin, however, did not block positive survival (P>0.05). reduced increased development follicles, but blocked by (P<0.05). Melatonin, influence number cells. In contrast, percentages tissues, rapamycin. Tissues CAT lower GPx when compared medium. conclusion, promotes activation, increases fiber vitro bovine tissue through its membrane-coupled mTORC1. On other hand, increase acting pathways, since it can pass membranes directly regulate oxidative stress.

Language: Английский

Citations

0