The potential role of seminal extracellular vesicles as biomarkers of male fertility and sperm cryotolerance in livestock species DOI Creative Commons
Sanchi Rana, Farooz Ahmad Lone, João Batista Freire de Souza

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(12)

Published: Nov. 17, 2024

Seminal extracellular vesicles are lipid bilayer membrane-bound that contain the important biomolecules of semen and function in intercellular communication reproductive tract. They secreted by male tract epithelium accessory sex glands particularly seminal vesicle prostate. The number plasma is greater than other body fluids, but least studied, especially livestock species. In vitro studies on influence spermatozoa functions have revealed their potential role improving quality preservation. Their as cryoprotectants cryopreservation a less developed area has good scope for research. SEVs isolated from various methods based principle size density gradient separation. Ultracentrifugation combined with ultracentrifugation most commonly used isolation method. Characterization done morphology, specific membrane markers (CD9and CD63), particle concentration. Many proteomic transcriptomic compositional differences between fertile sub-fertile populations. This provides hope utilizing these noninvasive diagnostic biomarkers fertility. ability to protect cargo phagocytosis degradation makes them more relevant reliable biomarkers. review discusses summarizes information available applications Also micro RNAs protein show altered expression pathological infertility conditions. Hence level could be fertility

Language: Английский

Swine in vitro embryo production: Potential, challenges, and advances DOI Creative Commons
Manuela Garcia-Canovas, Inmaculada Parrilla, Cristina Cuello

et al.

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 270, P. 107600 - 107600

Published: Sept. 12, 2024

Language: Английский

Citations

6
Small and Large Extracellular Vesicles of Porcine Seminal Plasma Differ in Lipid Profile DOI Open Access
Pablo Martínez‐Díaz, Ana Parra, Christian M. Sánchez‐López

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7492 - 7492

Published: July 8, 2024

Seminal plasma contains a heterogeneous population of extracellular vesicles (sEVs) that remains poorly characterized. This study aimed to characterize the lipidomic profile two subsets differently sized sEVs, small (S-) and large (L-), isolated from porcine seminal by size-exclusion chromatography characterized an orthogonal approach. High-performance liquid chromatography-high-resolution mass spectrometry was used for analysis. A total 157 lipid species 14 classes 4 major categories (sphingolipids, glycerophospholipids, glycerolipids, sterols) were identified. Qualitative differences limited cholesteryl ester present only in S-sEVs. L-sEVs had higher levels all quantified due their larger membrane surface area. The distribution pattern different, especially sphingomyelins (more S-sEVs) ceramides L-sEVs). In conclusion, this reveals suggesting they differ biogenesis functionality.

Language: Английский

Citations

4
Isolation and Characterization of Extracellular Vesicle Subsets in Donkey Seminal Plasma DOI
Jordi Miró, Jaime Catalán, Pablo Martínez‐Díaz

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Protective role of extracellular vesicles against oxidative DNA damage DOI Creative Commons
Jordi Ribas‐Maynou, Ana Parra, Pablo Martínez‐Díaz

et al.

Biological Research, Journal Year: 2025, Volume and Issue: 58(1)

Published: March 13, 2025

Abstract Background Oxidative stress, a source of genotoxic damage, is often the underlying mechanism in many functional cell disorders. Extracellular vesicles (EVs) have been shown to be key regulators cellular processes and may involved maintaining redox balance. Herein, we aimed develop method assess effects EVs on DNA oxidation using porcine seminal plasma extracellular (sEVs). Results The technique was set cell-free plasmid avoid bias generated by uptake sEVs sperm cells, employing increasing concentrations hydrogen peroxide (H 2 O ) that generate single-strand breaks (SSBs). Because SSBs contain free 3’-OH end allow extension through quantitative PCR, such -and therefore SYBR intensity- showed proportional amount SSB. In next stage, H co-incubated with two size-differentiated subpopulations (small large) permeabilized non-permeabilized whether intravesicular content (IC) or surface protects from oxidative damage. obtained small reduced incidence ( P < 0.05), whereas large had no impact generation > 0.05). IC protective effect against Conclusions Our results suggest sEVs, including peripheral corona layer, exert function alterations are originated mechanisms. addition, our vitro study opens path detect, localize quantify derived different fluids, elucidating their physiopathological states.

Language: Английский

Citations

0
Updating Research on Extracellular Vesicles of the Male Reproductive Tract in Farm Animals: A Systematic Review DOI Creative Commons
Pablo Martínez‐Díaz, Ana Parra,

Marina Montesdeoca

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(21), P. 3135 - 3135

Published: Oct. 31, 2024

This systematic review examined research studies on extracellular vesicles (EVs) of the male reproductive tract in livestock species to summarize topics and methodologies used, key findings, future directions. PubMed Scopus were searched for time ranges up 1 September 2024, 1383 articles identified. The application screening eligibility criteria resulted selection 79 focusing EVs livestock. Porcine bovine most studied. A variety EV isolation techniques with ultracentrifugation being common. Characterization was a weak point, only 24.05% characterizing according MISEV guidelines. Inadequate characterization compromises reliability results. results 19 that provided good showed from are phenotypically compositionally heterogeneous. These papers also these would be involved regulation sperm functionality. Research remains scarce, further is needed, which should include appropriate aim find efficient methods isolate them assess their involvement functionality spermatozoa cells female genital tract.

Language: Английский

Citations

2
Analysis of stallion spermatozoa metabolism using Agilent Seahorse XFp Technology DOI Creative Commons
José M. Ortiz-Rodríguez, Diego Bucci,

Laura Tovar-Pascual

et al.

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 271, P. 107633 - 107633

Published: Nov. 3, 2024

Language: Английский

Citations

2
The role of reproductive tract extracellular vesicles on boar sperm function DOI
Zhiqian Xu,

Ke Zhang,

Youbing Yang

et al.

Theriogenology, Journal Year: 2024, Volume and Issue: 230, P. 278 - 284

Published: Sept. 27, 2024

Language: Английский

Citations

1
RNA profiles differ between small and large extracellular vesicle subsets isolated from porcine seminal plasma DOI Creative Commons
Isabel Barranco, C. Almiñana, Ana Parra

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Dec. 27, 2024

Abstract Background Extracellular vesicles (EVs) are essential for cell-to-cell communication because they transport functionally active molecules, including proteins, RNA, and lipids, from secretory cells to nearby or distant target cells. Seminal plasma contains a large number of EVs (sEVs) that phenotypically heterogeneous. The aim the present study was identify RNA species contained in two subsets porcine sEVs different sizes, namely small (S-sEVs) (L-sEVs). were isolated 54 seminal samples by method combining serial centrifugations, size exclusion chromatography, ultrafiltration. characterized using an orthogonal approach. Analysis content quantification performed RNA-seq analysis. Results had distributions ( P < 0.001). They also showed differences concentration, morphology, specific protein markers 0.05). A total 735 RNAs identified quantified, which included: (1) mRNAs, rRNAs, snoRNAs, snRNAs, tRNAs, other ncRNAs (termed as “all RNAs”), (2) miRNAs (3) piRNAs. distribution pattern these classes differed between S-sEVs L-sEVs More than half RNAs”, piRNAs found be differentially abundant S- (FDR 0.1%). Among RNAs, RNAs” more L- S-sEVs, whereas most L-sEVs. Differentially equally distributed Some all associated with sperm quality functionality male fertility success. Conclusions Small show quantitative content. These would suggest each sEV subtype exerts functional activities targeted cells, spermatozoa female reproductive tract.

Language: Английский

Citations

1
The potential role of seminal extracellular vesicles as biomarkers of male fertility and sperm cryotolerance in livestock species DOI Creative Commons
Sanchi Rana, Farooz Ahmad Lone, João Batista Freire de Souza

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(12)

Published: Nov. 17, 2024

Seminal extracellular vesicles are lipid bilayer membrane-bound that contain the important biomolecules of semen and function in intercellular communication reproductive tract. They secreted by male tract epithelium accessory sex glands particularly seminal vesicle prostate. The number plasma is greater than other body fluids, but least studied, especially livestock species. In vitro studies on influence spermatozoa functions have revealed their potential role improving quality preservation. Their as cryoprotectants cryopreservation a less developed area has good scope for research. SEVs isolated from various methods based principle size density gradient separation. Ultracentrifugation combined with ultracentrifugation most commonly used isolation method. Characterization done morphology, specific membrane markers (CD9and CD63), particle concentration. Many proteomic transcriptomic compositional differences between fertile sub-fertile populations. This provides hope utilizing these noninvasive diagnostic biomarkers fertility. ability to protect cargo phagocytosis degradation makes them more relevant reliable biomarkers. review discusses summarizes information available applications Also micro RNAs protein show altered expression pathological infertility conditions. Hence level could be fertility

Language: Английский

Citations

0