RAPID GENOTYPING OF PRNP GENE POLYMORPHISMS TO ASSESS SCRAPIE SUSCEPTIBILITY IN BRAZILIAN SHEEP DOI Creative Commons
Camila Souza Rodrigues, Danielle Assis de Faria, K.M. Silva

et al.

Revista de Gestão Social e Ambiental, Journal Year: 2024, Volume and Issue: 18(12), P. e010511 - e010511

Published: Dec. 26, 2024

Objective: Our study aimed to develop and validate a low-density SNP panel for 15 PRNP gene polymorphisms genotyping sheep from breeds in Brazil, incorporating samples the Brazilian Animal Germplasm Biobank (BBGA). It was estimated both allelic, genotypic haplotypic frequencies selected markers. Theoretical Framework: The hypothesis centers on need cost-effective reduced panel, enhance accuracy, support selective breeding against scrapie sheep. Method: methodology adopted comprises with markers associated scrapie, designed genotype DNA of breeds. Results Discussion: achieved success rate 73%, as out genotyped markers, only four were excluded by call rate. Moreover, 73% (845) initial 1,152 successfully genotyped. Analyzing three most important polymorphisms, we found that ARQ/ARQ, susceptibility frequent all In contrast, ARR/ARR (resistance) BBGA (4%). Research Implications: Overall, can be rapid, dependable, tool enhancing genetic selection However, further technology optimization is necessary, which excluding replacing non-functional Originality/Value: This presents valuable insights providing cost-effective, validated tailored sheep, streamline programs.

Language: Английский

The Evolution of Conservation Biobanking: A Literature Review and Analysis of Terminology, Taxa, Location, and Strategy of Wildlife Biobanks Over Time DOI
Devin M. Chen, Gabriela F. Mastromonaco

Biopreservation and Biobanking, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Nearly one-third of flora, fauna, and funga species on Earth are threatened with extinction. In response, the prevalence repositories-often called "biobanks" or "genome resource banks"-for storing biological materials from has become more widespread. This research examined trends for (1) terminology, (2) taxa representation, (3) global distribution, (4) operational approach biobanks versus genome banks relating to zoos wildlife. Our literature search results indicate that although banking began earlier in 1990s, biobanking seen a surge publications over 3.5× since 2020. Genome bank articles were highly focused mammals (68%), while multi-taxonomic overviews less-studied taxa. parameters found largest number wildlife Europe (18) lowest South America (2), though likely impacted by being completed English. Additionally, only 28% (7/25) biodiversity hotspots contain biobank based our methodology. While not all efforts published reported, these findings suggest "biobank" will be widely used term future, is needed non-mammalian taxa, there geographical gaps biobanks, conservation programs should focus biospecimens wide set individuals develop assisted reproductive technologies concomitantly goal maintaining healthy, sustainable populations long term.

Language: Английский

Citations

0
Human and wildlife biobanks of germplasms and reproductive tissues can contribute to a broader concept of One Health DOI Creative Commons
Mayako Fujihara, Pierre Comizzoli

F&S Reports, Journal Year: 2025, Volume and Issue: 6, P. 63 - 66

Published: April 1, 2025

Language: Английский

Citations

0
Approaching the mystery of aging by the epigenetic clock DOI Creative Commons
Miho Inoue‐Murayama

Primates, Journal Year: 2024, Volume and Issue: 65(5), P. 359 - 363

Published: Aug. 14, 2024

Language: Английский

Citations

0
RAPID GENOTYPING OF PRNP GENE POLYMORPHISMS TO ASSESS SCRAPIE SUSCEPTIBILITY IN BRAZILIAN SHEEP DOI Creative Commons
Camila Souza Rodrigues, Danielle Assis de Faria, K.M. Silva

et al.

Revista de Gestão Social e Ambiental, Journal Year: 2024, Volume and Issue: 18(12), P. e010511 - e010511

Published: Dec. 26, 2024

Objective: Our study aimed to develop and validate a low-density SNP panel for 15 PRNP gene polymorphisms genotyping sheep from breeds in Brazil, incorporating samples the Brazilian Animal Germplasm Biobank (BBGA). It was estimated both allelic, genotypic haplotypic frequencies selected markers. Theoretical Framework: The hypothesis centers on need cost-effective reduced panel, enhance accuracy, support selective breeding against scrapie sheep. Method: methodology adopted comprises with markers associated scrapie, designed genotype DNA of breeds. Results Discussion: achieved success rate 73%, as out genotyped markers, only four were excluded by call rate. Moreover, 73% (845) initial 1,152 successfully genotyped. Analyzing three most important polymorphisms, we found that ARQ/ARQ, susceptibility frequent all In contrast, ARR/ARR (resistance) BBGA (4%). Research Implications: Overall, can be rapid, dependable, tool enhancing genetic selection However, further technology optimization is necessary, which excluding replacing non-functional Originality/Value: This presents valuable insights providing cost-effective, validated tailored sheep, streamline programs.

Language: Английский

Citations

0