Retinoblastoma-related (RBR) has both canonical and non-canonical regulatory functions during thermo-morphogenic responses in Arabidopsis seedlings DOI Creative Commons

Rasik Shiekh Bin Hamid,

Fruzsina Nagy,

Nikolett Kaszler

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 31, 2024

Abstract Warm temperatures accelerate plant growth, but the underlying molecular mechanism is not fully understood. Here, we show that increasing temperature from 22°C to 28°C rapidly activates proliferation in apical shoot and root meristems of wild-type Arabidopsis seedlings. We found one central regulators cell proliferation, cycle inhibitor RETINOBLASTOMA-RELATED (RBR), suppressed by warm temperatures. RBR became hyper-phosphorylated at a conserved CYCLIN-DEPENDENT KINASE (CDK) site young seedlings growing 28°C, parallel with stimulation expressions regulatory CYCLIN D/A subunits CDK(s). Interestingly, while under ectopic slowed down acceleration it triggered elongation growth post-mitotic cells hypocotyl. In agreement, genes thermomorphogenic response, including PIF4 PIF7 , as well their downstream auxin biosynthetic YUCCA ( YUC1-2 YUC8-9 ) were all up-regulated expressing line down-regulated mutant reduced level. suggest has both canonical non-canonical functions control proliferative respectively.

Language: Английский

Plants and global warming: challenges and strategies for a warming world DOI

Pratyay Seth,

José Sebastián

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(1)

Published: Jan. 1, 2024

Language: Английский

Citations

21
How Do Arabidopsis Seedlings Sense and React to Increasing Ambient Temperatures? DOI Creative Commons
Attila Fehér,

Rasik Shiekh Bin Hamid,

Zoltán Magyar

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(2), P. 248 - 248

Published: Jan. 16, 2025

Plants respond to higher ambient temperatures by modifying their growth rate and habitus. This review aims summarize the accumulated knowledge obtained with Arabidopsis seedlings grown at normal elevated temperatures. Thermomorphogenesis in shoot root is overviewed separately, since experiments indicate differences key aspects of thermomorphogenesis two organs. includes variances thermosensors transcription factors, as well predominance cell elongation or division, respectively, even though auxin plays a role regulating this process both Recent findings also highlight meristems suggest that cycle inhibitor RETINOBLASTOMA-RELATED protein may balance division increased

Language: Английский

Citations

0
Nitric oxide-mediated thermomemory: a new perspective on plant heat stress resilience DOI Creative Commons

Sheeba Naaz,

Anjali Pande, Ashverya Laxmi

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 28, 2025

In the intricate world of plant responses to environmental stress, concept thermomemory has emerged as a fascinating and complex phenomenon. Plants, sessile organisms, continually face challenge adapting fluctuating climates, ability “remember” prior heat stress encounters, phenomenon known is testament their remarkable adaptability. Nitric oxide (NO), versatile signaling molecule in physiology, been implicated myriad cellular processes crucial for adaptation. From its involvement stomatal regulation influence on gene expression antioxidant defense mechanisms, NO emerges central orchestrator plant’s response elevated temperatures. Exploration NO-mediated pathways provides insights into how plants not only cope with immediate but also retain memory these encounters. Unraveling molecular intricacies NO’s enhances our understanding sophisticated strategies employed by navigate changing climate, offering potential avenues innovative approaches enhancing crop resilience sustainable agriculture.

Language: Английский

Citations

0
ERF transcription factor regulons underpin growth-defence trade-off under acute heat stress in rice seedlings DOI Creative Commons
Akshay U. Nair,

Shubham Vishwakarma,

Titir Guha

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Summary Rice, a staple cereal crop, faces significant threats from rising temperatures, affecting all growth stages including early seedling establishment. Despite being critical in determining overall and productivity, response to heat stress during the stage remains understudied. This research aimed assess impact of acute on rice seedlings unravel underlying molecular mechanisms. Rice were exposed varying intensities durations determine threshold growth. To elucidate transcription factor (TF)-mediated regulatory mechanisms their functional interactome stress, transcriptomic analysis shoots roots was performed. Transcriptome unveiled comprehensive TF-target map for roots, potentially involved modulation growth-defence trade-off stress. Ethylene Responsive Factors (ERFs) emerged as central regulators, with phytohormones ethylene jasmonic acid acting upstream modulators. Pre-treatment these alleviated adverse effects study uncovers key governing responses involving ERFs-hormonal interactions. Modulating core regulators presents promising strategy enhance resilience, addressing global food security amid temperatures.

Language: Английский

Citations

0
RtHSFA9s of Rhodomyrtus tomentosa Positively Regulate Thermotolerance by Transcriptionally Activating RtHSFA2s and RtHSPs DOI Creative Commons
Huiguang Li, Ling Yang, Yujie Fang

et al.

Life, Journal Year: 2024, Volume and Issue: 14(12), P. 1591 - 1591

Published: Dec. 2, 2024

Heat shock transcription factors (HSFs) are crucial components in heat stress response. However, the contribution of HSFs governing inherent thermotolerance Rhodomyrtus tomentosa has barely been investigated. We here compared roles RtHSFA9a, RtHSFA9b, and RtHSFA9c tolerance. These three genes results gene duplication events, but there exist vast variations their amino acid sequences. They all localized to nucleus. Arabidopsis thaliana plants with overexpressed RtHSFA9a outperformed wild-type plants, while over-accumulation RtHSFA9b had little impact on plant thermotolerance. By transiently overexpressing R. seedlings, mRNA abundance response genes, including RtHSFA2a, RtHSFA2b, RtHSP17.4, RtHSP21.8, RtHSP26.5, RtHSP70, were upregulated. Transactivation assays confirmed that regulatory divergences among these viz., highest activity regulating RtHSP70; can transcriptionally activate makes limited contributions accumulation RtHSP70. Our indicate RtHSFA9 make thermal adaption by positively RtHSP which provides novel insights into subfamily.

Language: Английский

Citations

2
RETINOBLASTOMA‐RELATED Has Both Canonical and Noncanonical Regulatory Functions During Thermo‐Morphogenic Responses in Arabidopsis Seedlings DOI Creative Commons

Rasik Shiekh Bin Hamid,

Fruzsina Nagy,

Nikolett Kaszler

et al.

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

ABSTRACT Warm temperatures accelerate plant growth, but the underlying molecular mechanism is not fully understood. Here, we show that increasing temperature from 22°C to 28°C rapidly activates proliferation in apical shoot and root meristems of wild‐type Arabidopsis seedlings. We found one central regulators cell proliferation, cycle inhibitor RETINOBLASTOMA‐RELATED (RBR), suppressed by warm temperatures. RBR became hyper‐phosphorylated at a conserved CYCLIN‐DEPENDENT KINASE (CDK) site young seedlings growing 28°C, parallel with stimulation expressions regulatory CYCLIN D/A subunits CDK(s). Interestingly, while under ectopic slowed down acceleration it triggered elongation growth post‐mitotic cells hypocotyl. In agreement, genes thermomorphogenic response, including PIF4 PIF7 , as well their downstream auxin biosynthetic YUCCA ( YUC1‐2 YUC8‐9 ) were all up‐regulated expressing line down‐regulated mutant reduced level. suggest has both canonical non‐canonical functions control proliferative respectively.

Language: Английский

Citations

1
Retinoblastoma-related (RBR) has both canonical and non-canonical regulatory functions during thermo-morphogenic responses in Arabidopsis seedlings DOI Creative Commons

Rasik Shiekh Bin Hamid,

Fruzsina Nagy,

Nikolett Kaszler

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 31, 2024

Abstract Warm temperatures accelerate plant growth, but the underlying molecular mechanism is not fully understood. Here, we show that increasing temperature from 22°C to 28°C rapidly activates proliferation in apical shoot and root meristems of wild-type Arabidopsis seedlings. We found one central regulators cell proliferation, cycle inhibitor RETINOBLASTOMA-RELATED (RBR), suppressed by warm temperatures. RBR became hyper-phosphorylated at a conserved CYCLIN-DEPENDENT KINASE (CDK) site young seedlings growing 28°C, parallel with stimulation expressions regulatory CYCLIN D/A subunits CDK(s). Interestingly, while under ectopic slowed down acceleration it triggered elongation growth post-mitotic cells hypocotyl. In agreement, genes thermomorphogenic response, including PIF4 PIF7 , as well their downstream auxin biosynthetic YUCCA ( YUC1-2 YUC8-9 ) were all up-regulated expressing line down-regulated mutant reduced level. suggest has both canonical non-canonical functions control proliferative respectively.

Language: Английский

Citations

0