Pan-PTM profiling identifies post-translational modifications associated with exceptional longevity and preservation of skeletal muscle function in Drosophila DOI Creative Commons

Suresh Poudel,

Chia-Lung Chuang, Him K. Shrestha

et al.

npj Aging, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 30, 2025

Skeletal muscle weakness is a major component of age-associated frailty, but the underlying mechanisms are not completely understood. Drosophila has emerged as useful model for studying skeletal aging. In this organism, previous lab-based selection established strains with increased longevity and reduced functional decline compared to parental strain. Here, we have applied computational pipeline (JUMPptm) retrieving information on 8 post-translational modifications (PTMs) from proteomes 2 long-lived corresponding strain in young old age. This pan-PTM analysis identified 2470 modified sites (acetylation, carboxylation, deamidation, dihydroxylation, mono-methylation, oxidation, phosphorylation, ubiquitination) several classes proteins, including evolutionarily conserved contractile proteins metabolic enzymes. PTM consensus sequences further highlight amino acids that enriched adjacent site, thus providing insight into flanking residues influence distinct PTMs. Altogether, these analyses identify PTMs associated during aging may underlie negligible senescence lab-evolved strains.

Language: Английский

Pan-PTM profiling identifies post-translational modifications associated with exceptional longevity and preservation of skeletal muscle function in Drosophila DOI Creative Commons

Suresh Poudel,

Chia-Lung Chuang, Him K. Shrestha

et al.

npj Aging, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 30, 2025

Skeletal muscle weakness is a major component of age-associated frailty, but the underlying mechanisms are not completely understood. Drosophila has emerged as useful model for studying skeletal aging. In this organism, previous lab-based selection established strains with increased longevity and reduced functional decline compared to parental strain. Here, we have applied computational pipeline (JUMPptm) retrieving information on 8 post-translational modifications (PTMs) from proteomes 2 long-lived corresponding strain in young old age. This pan-PTM analysis identified 2470 modified sites (acetylation, carboxylation, deamidation, dihydroxylation, mono-methylation, oxidation, phosphorylation, ubiquitination) several classes proteins, including evolutionarily conserved contractile proteins metabolic enzymes. PTM consensus sequences further highlight amino acids that enriched adjacent site, thus providing insight into flanking residues influence distinct PTMs. Altogether, these analyses identify PTMs associated during aging may underlie negligible senescence lab-evolved strains.

Language: Английский

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