A novel thin plate spline methodology to model tissue surfaces and quantify tumor cell invasion in organ-on-chip models DOI Creative Commons
Elizabeth Elton, Carly Strelez, Nolan Ung

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 21, 2023

ABSTRACT Organ-on-chip (OOC) models can be useful tools for cancer drug discovery. Advances in OOC technology have led to the development of more complex assays, yet analysis these systems does not always account advancements, resulting technical challenges. A challenging task two-channel microfluidic is define boundary between channels so objects moving within and quantified. We propose a novel imaging-based application thin plate spline method – generalized cubic that used model coordinate transformations tissue compartments quantification invaded objects, representing early steps metastasis. To evaluate its performance, we applied our analytical approach an adapted developed by Emulate, Inc., utilizing system with endothelial cells bottom channel colorectal (CRC) patient-derived organoids (PDOs) top channel. Initial visualization this revealed variations due microscope stage tilt ridge valley-like contours surface. The was functionalized into reproducible process web tool Chip Invasion Contour Analysis (ChICA) surface quantify invading tumor across multiple chips. illustrate applicability method, CRC organoid-chips seeded two different cell types measured distinct surfaces invasion dynamics. Since ChICA utilizes only positional data output from imaging software, applicable agnostic image used. variation introduced manufacturing or during experimental workflow, quickly accurately measure invasion, explore biological mechanisms

Language: Английский

A novel thin plate spline methodology to model tissue surfaces and quantify tumor cell invasion in organ-on-chip models DOI Creative Commons
Elizabeth Elton, Carly Strelez, Nolan Ung

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 21, 2023

ABSTRACT Organ-on-chip (OOC) models can be useful tools for cancer drug discovery. Advances in OOC technology have led to the development of more complex assays, yet analysis these systems does not always account advancements, resulting technical challenges. A challenging task two-channel microfluidic is define boundary between channels so objects moving within and quantified. We propose a novel imaging-based application thin plate spline method – generalized cubic that used model coordinate transformations tissue compartments quantification invaded objects, representing early steps metastasis. To evaluate its performance, we applied our analytical approach an adapted developed by Emulate, Inc., utilizing system with endothelial cells bottom channel colorectal (CRC) patient-derived organoids (PDOs) top channel. Initial visualization this revealed variations due microscope stage tilt ridge valley-like contours surface. The was functionalized into reproducible process web tool Chip Invasion Contour Analysis (ChICA) surface quantify invading tumor across multiple chips. illustrate applicability method, CRC organoid-chips seeded two different cell types measured distinct surfaces invasion dynamics. Since ChICA utilizes only positional data output from imaging software, applicable agnostic image used. variation introduced manufacturing or during experimental workflow, quickly accurately measure invasion, explore biological mechanisms

Language: Английский

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