Laser-assisted bioprinting of targeted cartilaginous spheroids for high density bottom-up tissue engineering

Biofabrication, Journal Year: 2024, Volume and Issue: 16(4), P. 045029 - 045029

Published: Aug. 12, 2024

Abstract Multicellular spheroids such as microtissues and organoids have demonstrated great potential for tissue engineering applications in recent years these 3D cellular units enable improved cell–cell cell–matrix interactions. Current bioprinting processes that use multicellular building blocks limited control on post printing distribution of cell or moderate throughput efficiency. In this work, we presented a laser-assisted approach able to transfer larger structures. Cartilaginous formed by human periosteum derived cells (hPDCs) were successfully bioprinted possessing high viability the capacity undergo chondrogenic differentiation printing. Smaller hPDC with diameters ranging from ∼100 150 µ m through laser-induced forward method (LIFT) however constituted challenge. For reason novel alternative was developed termed laser induced propulsion mesoscopic objects (LIPMO) whereby bioprint up 300 m. Moreover, combined process computer aided image analysis demonstrating ‘target shoot’, automated selection, multiple large single sequence. By taking advantage target shoot system, multilayered constructs containing density fabricated.

Language: Английский

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Biotechnological advances in 3D modeling of cancer initiation. Examples from pancreatic cancer research and beyond

Biofabrication, Journal Year: 2025, Volume and Issue: 17(2), P. 022008 - 022008

Published: Feb. 28, 2025

In recent years, biofabrication technologies have garnered significant attention within the scientific community for their potential to create advancedin vitrocancer models. While these been predominantly applied model advanced stages of cancer, there exists a pressing need develop pertinent, reproducible, and sensitive 3D models that mimic cancer initiation lesions native tissue microenvironment. Such hold profound relevance comprehending intricacies initiation, devise novel strategies early intervention, and/or conduct sophisticated toxicology assessments putative carcinogens. Here, we will explain pivotal factors must be faithfully recapitulated when constructing models, with specific focus on pancreatic lesions. By synthesizing current state research in this field, provide insights into advances breakthroughs. Additionally, delineate key technological biological challenges necessitate resolution future endeavors, thereby paving way more accurate insightfulin

Language: Английский

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3D bioprinted thick hepatic constructs with vascular network as a physiologically relevant in vitro organ model

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101786 - 101786

Published: April 1, 2025

Language: Английский

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Technique of 3D printing for scaffolding in tissue engineering of bones: Opportunities and challenges

Materials Today Communications, Journal Year: 2024, Volume and Issue: 42, P. 111249 - 111249

Published: Dec. 9, 2024

Language: Английский

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