Sustainable keratoplasty models using agri-food waste: a hypothesis for transforming biowaste into biomaterials for tissue engineering research

Frontiers in Sustainable Food Systems, Journal Year: 2025, Volume and Issue: 9

Published: March 26, 2025

With a growing global population, ways to counterbalance the demand for meat products with effective food security and waste management innovative scalable solutions. Concurrently, alarming incidence of end-stage organ failure, limited availability transplantable organs, directives reduce reliance on animal testing underscore need clinically viable sustainable alternatives. Our approach introduces hypothesis-driven, renewable tissue engineering strategy that creates low-cost keratoplasty models derived entirely from agri-food waste. Specifically, we hypothesize abundant by-products, such as eyes bladders, provide practically unlimited readily available supplies corneal tissues urine-derived stem cells (USCs) can be repurposed into cost-effective, relevant Traditional approaches often rely cadaveric tissues, invasive cell sourcing, or expensive commercial lines, which require complex resource-intensive processes, including high-end bioreactor systems manufacturing environments. These requirements limit widespread adoption technological progress needed increase supply keratografts. proposed leverages combination post-mortem bladder harvesting, in turn facilitates decellularization, non-invasive USC differentiation, compartment-specific recellularization help overcome barriers associated traditional seeding generate this type Overall, our perspective suggests way devise transformative resource-efficient engineering, specifically geared toward improving outcomes while offering broader applications regeneration other bodily tissues/organs biotechnological innovation.

Language: Английский

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Bone Tissue Engineering: From Biomaterials to Clinical Trials

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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ATM knock out alters calcium signalling and augments contraction in skeletal muscle cells differentiated from human urine-derived stem cells

Cell Death Discovery, Journal Year: 2025, Volume and Issue: 11(1)

Published: April 15, 2025

Abstract Ataxia-telangiectasia (A-T) is a rare neurodegenerative disorder caused by the deficiency of serine/threonine kinase ataxia telangiectasia mutated (ATM) protein, whose loss function leads to altered cell cycle, apoptosis, oxidative stress balance and DNA repair after damage. The clinical manifestations are multisystemic, among them cerebellar degeneration muscular ataxia. molecular mechanism which ATM A-T still uncertain and, currently only symptomatic treatments available. In this study, we generated functional skeletal muscle model that recapitulates highlights role in calcium signalling contraction. To aim, using CRISPR/Cas9 technology, knocked out protein urine-derived stem cells (USCs) from healthy donors. resulting USCs-ATM-KO maintained stemness but showed G2/S cycle progression an inability UV Moreover, they increased cytosolic release ATP stimulation detriment mitochondria. alterations homoeostasis were differentiation into (USC-SkMCs) correlated with impaired Indeed, USC-SkMCs-ATM-KO contraction kinetics dramatically accelerated compared control cells. These results highlight relevant muscle, not dependent on non-functional neuronal communication, paving way for future studies interpretation

Language: Английский

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Effective Bone Tissue Fabrication Using 3D-Printed Citrate-Based Nanocomposite Scaffolds Laden with BMP9-Stimulated Human Urine Stem Cells

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 17(1), P. 197 - 210

Published: Dec. 24, 2024

Effective repair of large bone defects through tissue engineering (BTE) remains an unmet clinical challenge. Successful BTE requires optimal and synergistic interactions among biocompatible scaffolds, osteogenic factors, osteoprogenitors to form a highly vascularized microenvironment for regeneration osseointegration. We sought develop effective system by using 3D printed citrate-based mPOC/hydroxyapatite (HA) composites laden with BMP9-stimulated human urine stem cells (USCs). Specifically, we synthesized characterized methacrylate poly(1,8 octamethylene citrate) (mPOC), mixed it 0%, 40% or 60% HA (i.e., mPOC-0HA, mPOC-40HA, mPOC-60HA), fabricated composite scaffold via micro-continuous liquid interface production (μCLIP). The 3D-printed mPOC-HA scaffolds were compatible USCs that exhibited high activity in vitro upon BMP9 stimulation. Subcutaneous implantation revealed formation all three types scaffolds. Histologic evaluation the mPOC-60HA yielded most mature bone, resembling native extensive scaffold-osteointegration. Collectively, these findings demonstrate composite, cells, potent factor constitute desirable triad engineering.

Language: Английский

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