Co-targeting of glial activation and inflammation by tsRNA-Gln-i-0095 for treating retinal ischemic pathologies DOI Creative Commons
Ying Zhang, Yan Ma,

Yu‐Ke Ji

et al.

Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 10, 2025

Ischemic retinopathies are the major causes of blindness, yet effective early-stage treatments remain limited due to an incomplete understanding underlying molecular mechanisms. Significant changes in gene expression often precede structural and functional alterations. Transfer RNA (tRNA)-derived small RNAs (tsRNAs) emerging as novel regulators, involved various biological processes human diseases. In this study, tsRNA-Gln-i-0095 was identified a regulator, which significantly upregulated retinal ischemia/reperfusion (I/R) injury. Reducing levels suppressed reactive gliosis, lowered inflammatory cytokine levels, protected ganglion cells from I/R These effects led reduced damage, inhibited glial activation inflammation, enhanced neuronal function. Mechanistically, downregulated NFIA TGFBR2 through miRNA-like mechanism. Collectively, study highlights potential targeting therapeutic approach reduce injury preserve visual

Language: Английский

Nanoparticle-Based gene therapy strategies in retinal delivery DOI
Thomas S. Foster, Patrick J. Lim, Susbin Raj Wagle

et al.

Journal of drug targeting, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 20

Published: Jan. 3, 2025

Vision loss and blindness are significant issues in both developed developing countries. There a wide variety of aetiologies that can cause vision loss, which outlined this review. Although treatment has significantly improved over time for some conditions, nearly half all people with impairment left untreated. Gene delivery is an emerging field may assist the these difficult to manage forms loss. Here we review how component nanotechnology-based, non-viral gene systems being applied help resolve impairment. This focuses on use lipid polymer nanoparticles, quantum dots as vectors eye. Finally, also highlight technologies be useful discipline.

Language: Английский

Citations

0
A review on eye diseases induced by blue light: pathology, model, active ingredients and mechanisms DOI Creative Commons
Yan Yuan, Yibo Wu, Yu Zhao

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 23, 2025

Blue light induced eye damage (BLED) belongs to modern diseases. It is an ophthalmic disease caused by prolonged exposure electronic devices or screens containing a large amount of high-energy short waves (blue light). Specific symptoms include dryness and discomfort in the eyes, blurred vision, headache, insomnia, severe cases, it may also cause various diseases such as cataracts glaucoma. At present, development health products drugs for blue injury faces many difficulties. Therefore, further exploration research are needed on pathogenesis, pathophysiology, pharmacological mechanisms injury. Natural medicine ingredients preparations have unique advantages targeting fatigue due their multi-component synergistic effects, overall regulation, mild safe characteristics. Starting from disease-related pathophysiological characteristics injury, this article elucidates treating same time, reviews vitro cultured cell animal model conditions order provide reference subsequent modeling experiments. And explore future directions new ideas methods prevention treatment BLED.

Language: Английский

Citations

0
Dual mRNA nanoparticles strategy for enhanced pancreatic cancer treatment and β-elemene combination therapy DOI Creative Commons

Qianru Zhu,

Changyuan Yu, Yiquan Chen

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(11)

Published: March 11, 2025

Pancreatic ductal adenocarcinoma (PDAC) is notoriously immune-resistant, limiting the clinical efficacy of single-agent immune modulators and thereby necessitating exploration multimodal immunotherapy combinations. Traditional approaches combining conventional checkpoint inhibitors with neoantigen vaccines have shown some promise in treating PDAC but are often compromised by intratumoral T lymphocyte exhaustion systemic toxicity. Hence, novel needed to address these challenges. Herein, we demonstrate that mRNA polymeric nanoparticles encoding anti-PD-1 antibodies situ at tumor site enhance therapeutic neoantigen-based vaccine for PDAC. This mRNA-based, antibody production strategy also protects tumor-infiltrating cells from PD-1 inhibition, potentially reducing toxicities induced inhibition. Our study may provide an innovative dual nanoparticle effective immunotherapy, as well cancer combination therapy other clinically approved drugs (e.g., β-elemene).

Language: Английский

Citations

0
Suture‐Free Corneal Transplantation via Bioself‐Adhesive Lenticules Based on Macromolecular Electrostatic Entanglement DOI Open Access

Zhe Zhang,

Yanze Yu,

Ding Zhao

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Abstract The corneal lenticules extracted from small incision lenticule extraction (SMILE) can be used as an optional transplantation material. However, it cannot for deep anterior lamellar keratoplasty due to its thickness limitations. In this study, oxidized chondroitin sulfate (O─CS) containing ─SO 3 − groups twisted with generation 4.0 polyamidoamine dendrimers (G4 PAMAM) which contain −NH 2 + via electrostatic entanglement form a cohesive module, is applicable in suture‐free of cornea, providing the possibility repairing defects. intermolecular enhanced self‐adhesion module (33.80 ± 2.12 kPa) high light transmittance (more than 70%) and excellent biocompatibility, benefits stable adhesion long term. Additionally, G4 PAMAM control long‐term puerarin drug release then inhibit postoperative inflammatory response. bioself‐adhesion between macromolecules realize tissues, provides promising strategy living tissue wound repair.

Language: Английский

Citations

0
Stimulus-responsive nanomaterials for ocular antimicrobial therapy DOI
Tao Zhang, Zichao Luo

Nanoscale, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review highlights recent stimulus-responsive antimicrobial nanotherapeutics for ocular infections, focusing on their design, progress, and advantages/limitations.

Language: Английский

Citations

0
Alternative routes for parenteral nucleic acid delivery and related hurdles: highlights in RNA delivery DOI
Joana Cunha, Fátima V. Ventura, Christine Charrueau

et al.

Expert Opinion on Drug Delivery, Journal Year: 2024, Volume and Issue: 21(10), P. 1415 - 1439

Published: Sept. 14, 2024

Nucleic acid-based therapies are promising advancements in medicine. They offer unparalleled efficacy treating previously untreatable diseases through precise gene manipulation techniques. However, the challenge of achieving targeted delivery to specific cells remains a significant obstacle.

Language: Английский

Citations

3
Co-targeting of glial activation and inflammation by tsRNA-Gln-i-0095 for treating retinal ischemic pathologies DOI Creative Commons
Ying Zhang, Yan Ma,

Yu‐Ke Ji

et al.

Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 10, 2025

Ischemic retinopathies are the major causes of blindness, yet effective early-stage treatments remain limited due to an incomplete understanding underlying molecular mechanisms. Significant changes in gene expression often precede structural and functional alterations. Transfer RNA (tRNA)-derived small RNAs (tsRNAs) emerging as novel regulators, involved various biological processes human diseases. In this study, tsRNA-Gln-i-0095 was identified a regulator, which significantly upregulated retinal ischemia/reperfusion (I/R) injury. Reducing levels suppressed reactive gliosis, lowered inflammatory cytokine levels, protected ganglion cells from I/R These effects led reduced damage, inhibited glial activation inflammation, enhanced neuronal function. Mechanistically, downregulated NFIA TGFBR2 through miRNA-like mechanism. Collectively, study highlights potential targeting therapeutic approach reduce injury preserve visual

Language: Английский

Citations

0