Lithium-doped calcium silicate cement regulates the immune microenvironment and promotes M2 macrophage polarization for enhancing bone regeneration

Journal of Biological Engineering, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 6, 2025

Bone defects present a significant challenge in orthopedics and trauma surgery, necessitating innovative approaches to stimulate effective bone regeneration. This study investigated the potential of lithium-doped calcium silicate (LiCS) cement enhance regeneration modulate immune microenvironment promote tissue repair. We synthesized LiCS ceramic powder performed comprehensive analyses its physicochemical properties, including phase composition, morphology, setting time, mechanical strength. The results demonstrated that incorporation lithium into significantly increased diametral tensile strength (DTS) facilitated hydroxyapatite formation compared with undoped silicate. In vitro assays revealed enhanced proliferation, adhesion, spread Wharton's jelly mesenchymal stem cells (WJMSCs). Additionally, Li-CS exhibited remarkable immunomodulatory properties by reducing pro-inflammatory cytokines increasing anti-inflammatory cytokines, promoting polarization macrophages towards M2 phenotype. presence Li also improved osteogenic differentiation WJMSCs, as evidenced elevated levels alkaline phosphatase osteocalcin expression. These findings underscore dual functional capabilities enhancing osteogenesis modulating environment, making it promising material for engineering

Language: Английский

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Modelling of macrophage responses to biomaterials in vitro: state-of-the-art and the need for the improvement

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: March 26, 2024

The increasing use of medical implants in various areas medicine, particularly orthopedic surgery, oncology, cardiology and dentistry, displayed the limitations long-term integration available biomaterials. effective functioning successful requires not only technical excellence materials but also consideration dynamics biomaterial interaction with immune system throughout entire duration implant use. acute as well decisions about efficiency are done by local resident tissue macrophages monocyte-derived that start to be recruited during damage, when is installed, continuously healing phase. Our review summarized knowledge currently used macrophages-based vitro cells include murine human lines primary ex vivo differentiated macrophages. We provided information most frequently examined biomarkers for inflammation, chronic foreign body response fibrosis, indicating benefits model systems. Particular attention given scavenging function controls dynamic composition peri-implant microenvironment ensures timely clearance microorganisms, cytokines, metabolites, extracellular matrix components, dying debris. outline perspective application 3D systems modelling tissue-specific avoiding animal experimentation.

Language: Английский

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TNF-α-licensed exosome-integrated titanium accelerated T2D osseointegration by promoting autophagy-regulated M2 macrophage polarization

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 727, P. 150316 - 150316

Published: June 25, 2024

Language: Английский

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Immunomodulatory and bone regenerative properties of copper/procyanidins-modified titanium surfaces

Biomaterials Advances, Journal Year: 2025, Volume and Issue: 169, P. 214199 - 214199

Published: Jan. 23, 2025

Language: Английский

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Multifunctional Micro/Nano-Textured Titanium with Bactericidal, Osteogenic, Angiogenic and Anti-inflammatory Properties: Insights from In Vitro and In Vivo Studies

Materials Today Bio, Journal Year: 2025, Volume and Issue: 32, P. 101710 - 101710

Published: March 29, 2025

Titanium (Ti) is widely used as an implantable material for bone repair in orthopedics and dentistry. However, Ti implants are vulnerable to bacterial infections, which can compromise patient recovery lead implant failure. While a controlled inflammatory response promotes regeneration, chronic inflammation caused by infections Bone complex process inflammation, angiogenesis osteogenesis tightly interconnected, requiring cooperation between mesenchymal stem cells (MSC), macrophages endothelial cells. Here, we fabricated bio-inspired with either microstructured (Micro Ti) or nanostructured (Nano surface textures that exhibit robust mechano-bactericidal properties. In vitro, both textured surfaces improved blood coagulation osteogenic marker expression compared smooth surfaces. Additionally, Nano promoted macrophage polarization towards the M2 phenotype enhanced paracrine effects of MSCs on angiogenesis, key processes tissue regeneration. vivo kinetic analysis reconstruction rat calvarial model showed osseointegration, evidenced increased volume, mineral density, bone-implant contact. Notably, Micro no significant differences from control implants. These findings highlight potential nanopatterns simultaneously prevent enhance osseointegration modulating protein adsorption, osteogenesis. This study provides new insights into development bifunctional implants, offering perspectives next generation bone-related biomaterials.

Language: Английский

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Osteoimmunomodulatory biomaterials: Engineering strategies, current progress, and future perspectives for bone regeneration

Applied Materials Today, Journal Year: 2025, Volume and Issue: 44, P. 102753 - 102753

Published: April 29, 2025

Language: Английский

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Timely delivery of bone marrow mesenchymal stem cells based on the inflammatory pattern of bone injury environment to promote the repair of calvarial bone defects in rats: An optimized strategy for bone tissue engineering

Journal of Tissue Engineering, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 1, 2024

Stem cell-based therapy plays a significant role in the repair of bone defects. However, traditional stem cell transplantation strategies tissue engineering are characterized by low survival rates and unstable treatment outcomes. In this study, we propose timely delivery strategy for inflammatory changes setting injury to improve rate transplanted cells repair. The results tracing vivo showed that could effectively low-dose exogenous defect areas, CD31 immunofluorescence histological sections suggested promoted vascularization new formation calvarial area. Subsequently, analyzed mechanism action “Two-step” from perspective microenvironment regulation, first batch caused localized transient increases apoptosis levels factors, recruited macrophage chemotaxis, second may promote pro-inflammatory - anti-inflammatory transformation tissue. Finally, mRNA sequencing suggest important initiators repair, which not only actively regulate immune at defect, but also guide richer cellular activity more positive biochemical responses. Therefore, leads efficient environment regulation superior effects, provide an alternative option defects future.

Language: Английский

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Inflammatory or Reparative? Tuning Macrophage Polarization Using Anodized Anisotropic Nanoporous Titanium Implant Surfaces

Small Science, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 17, 2024

Modulating macrophage phenotype based on implant surface characteristics, including topography and chemistry, has been employed to enhance osseointegration long‐term functional outcomes for titanium (Ti)‐based implants. An excessive and/or prolonged M1 response can lead damaging immune‐inflammatory reactions, negatively influencing the fate of implant, hence, modulating these responses via nanoscale modification is an emerging paradigm. Herein, anodized single‐step electrochemical anodization, with preserved underlying microfeatures superimposed nanopores (50 70 nm), compared irregular rough microrough (machined‐like) surfaces, investigated its effect functions primary macrophages in vitro. Significantly reduced proliferation increased tissue‐reparative M2 polarization are confirmed nanopores, which more pronounced nm diameter. Moreover, osteoclastogenesis while osteogenic differentiation osteoblasts enhanced (higher pores). Advanced nanoengineered Ti implants tissue integration by inflammatory at implant–cell interface.

Language: Английский

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Lithium-doped calcium silicate regulates the immune microenvironment and promotes M2 macrophage polarization for enhancing bone regeneration

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 11, 2024

Bone defects present a significant challenge in orthopedics and trauma surgery, necessitating innovative approaches to stimulate effective bone regeneration. This study investigated the potential of lithium-doped calcium silicate (LiCS) cement enhance regeneration modulate immune microenvironment promote tissue repair. We synthesized LiCS ceramic powder performed comprehensive analyses its physicochemical properties, including phase composition, morphology, setting time, mechanical strength. The results demonstrated that incorporation lithium into significantly increased diametral tensile strength (DTS) facilitated hydroxyapatite formation compared with undoped silicate. In vitro assays revealed enhanced proliferation, adhesion, spread Wharton's jelly mesenchymal stem cells (WJMSCs). Additionally, Li-CS exhibited remarkable immunomodulatory properties by reducing pro-inflammatory cytokines increasing anti-inflammatory cytokines, promoting polarization macrophages towards M2 phenotype. presence Li also improved osteogenic differentiation WJMSCs, as evidenced elevated levels alkaline phosphatase osteocalcin expression. These findings underscore dual functional capabilities enhancing osteogenesis modulating environment, making it promising material for engineering

Language: Английский

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Macrophages and the musculoskeletal system

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 99 - 168

Published: Jan. 1, 2024

Language: Английский

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