The impact of ischemic stroke on bone marrow microenvironment and extracellular vesicles: A study on inflammatory and molecular changes

Experimental Neurology, Journal Year: 2024, Volume and Issue: 379, P. 114867 - 114867

Published: June 22, 2024

An ischemic stroke (IS) is caused due to the lack of blood flow cerebral tissue. Most studies have focused on how affects localized tissue, but it has been observed that a can cause secondary complications in distant organs, such as Bone Marrow (BM). Our study effect strokes bone marrow microenvironment. (BM) vital organ maintains inflammatory homeostasis and aids repair damaged tissue after injury/IS. We used middle artery occlusion (MCAO) model adult mice (6 months) investigated changes BM environment. cells were for western blot RT-PCR, supernatant was cytokine analysis extracellular vesicle (EVs) isolation. significant increase total cell number within an TNF-alpha MCP-1, which are known inducing pro-inflammatory Western blots whole lysate demonstrated elevated levels factors (IL-6, TNF-alpha, TLR-4) senescence markers (p21 p16). EVs isolated from showed no change size or concentration; however, we found carried increased miRNA-141-3p miRNA-34a. Proteomic BM-derived alteration protein cargo IS. FgB, C3, Fn1, Tra2b levels. The signaling pathway mitochondrial function most affected marrow. IS induces environment secreted BM.

Language: Английский

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Stem cell-derived exosomes for ischemic stroke: a conventional and network meta-analysis based on animal models

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 23, 2024

Objective We aimed to evaluate the efficacy of stem cell-derived exosomes for treating ischemic stroke and screen optimal administration strategy. Methods searched PubMed, Web Science, Embase, Cochrane Library, Scopus databases relevant studies published from their inception 31 December 2023. Conventional network meta-analyses routes administration, types, immune compatibility were performed using cerebral infarct volume (%) modified neurological severity score (mNSS) as outcome indicators. Results A total 38 randomized controlled animal experiments included. meta-analysis showed that compared with negative control group: intravenous significantly reduced mNSS; intranasal (%); intracerebral mNSS. Adipose-derived mesenchymal (ADSC-Exos), bone marrow (BMSC-Exos), dental pulp (DPSC-Exos) neural (NSC-Exos) Endothelial progenitor (EPC-Exos), embryonic (ESC-Exos), induced pluripotent (iPSC-Exos) (NPC-Exos) Umbilical cord (UCMSC-Exos) there was no significant difference between urogenital (USC-Exos) controls. Engineered had better than unmodified exosomes. Both allogeneic xenogeneic The best route reducing Among 10 types administered intravenously, BMSC-Exos type Allogeneic in (%), whereas Conclusion This meta-analysis, by integrating available evidence, revealed is are exosome type, have mNSS, which can provide options preclinical studies. In future, more high-quality experiments, especially direct comparative needed determine strategy stroke. Systematic Review Registration: https://www.crd.york.ac.uk/PROSPERO/display_record.php?ID=CRD42024497333 , PROSPERO, CRD42024497333

Language: Английский

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Reveal more mechanisms of precondition mesenchymal stem cells inhibiting inflammation

World Journal of Stem Cells, Journal Year: 2024, Volume and Issue: 16(4), P. 459 - 461

Published: April 24, 2024

Hypoxia can get more ability to inhibit inflammation. But how it impact on survival time of mesenchymal stem cells (MSCs) is confusing and preconditioned MSCs inhibiting inflammation are partially known. Those issues decided the value by hypoxia.

Language: Английский

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Extracellular vesicles of ADSCs inhibit ischemic stroke-induced pyroptosis through Gbp3 regulation: A role for the NLRP3/GSDMD signaling pathway

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 146, P. 113881 - 113881

Published: Dec. 24, 2024

Language: Английский

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