Protective Effects of Bromelain in Testicular Torsion-Detorsion: Reducing Inflammation, Oxidative Stress, and Apoptosis While Enhancing Sperm Quality

Biomolecules, Journal Year: 2025, Volume and Issue: 15(2), P. 292 - 292

Published: Feb. 15, 2025

Inflammation and increased oxidative stress in testicular tissue are documented side effects of torsion the testicles. The preventive role Bromelain (Bro) against testicle torsion-induced ischemia/reperfusion (I/R) injury was investigated this research. Five groups six animals each were created: ischemia, Ischemia+Reperfusion (I+R), Ischemia+Reperfusion+Bromelain (I+R+Bro; 10 mg/kg), control (sham), (Bro; mg/kg). An I/R damage resulted from two hours 720° clockwise twisting left testis. Blood samples epididymal sperm collected after reperfusion to analyze parameters (recovery, motility, viability, morphology) cytokines that promote inflammation (IL-1β, IL-6, TNF-α). Using Western blotting, examined for histopathological alterations, antioxidant enzymes (GSH, SOD), lipid peroxidation (MDA), apoptosis, survival-related proteins (TLR4, Caspase-3, Bcl-2, NRF-2, HO-1, PI3K, mTOR, AKT-1). While raising activities GSH SOD, enzymes, Bro administration dramatically reduced MDA concentrations. I+R+Bro group had significantly amounts promoted compared I+R group. Bro's protective properties also attributed altered by it participate apoptosis survival cells. Sperm morphology, concentration notably improved bromelain-treated group, according spermatological examination. Testicular treated with bromelain showed less histological evaluations than untreated These findings imply has anti-inflammatory, anti-apoptotic, qualities. It effectively reduces modulating PI3K/Akt/mTOR NRF-2/HO-1 pathways, hence minimizing injury.

Language: Английский

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Prophylactic Effects of Rhamnetin Flavonoid on Indomethacin‐Induced Gastric Ulceration by Modulating HSP 70/Bax, SOD/MDA and TNF‐α/IL‐10

Clinical and Experimental Pharmacology and Physiology, Journal Year: 2025, Volume and Issue: 52(4)

Published: Feb. 19, 2025

ABSTRACT Rhamnetin is a naturally occurring flavonoid compound found in many wild plant species and indigenous fruits. Despite its numerous biological potentials, such as anti‐inflammatory, antioxidant antimicrobial effects, there lack of literature elucidating gastroprotective action anticipating molecular mechanism. Natural products can be good alternative to overcome the side effects relapses associated with anti‐ulcer drugs. This study aims elucidate rhamnetin's acute toxicity using indomethacin ulceration model. Animals were arbitrarily divided into five groups: negative control group (A) positive (B), both treated 1% carboxymethyl cellulose; reference (C) receiving 20 mg/kg omeprazole; low‐dose (D) high‐dose (E) rhamnetin groups 30 60 mg/kg, respectively. After 1 h, rats Groups B–E subjected indomethacin‐induced ulceration. Toxicity evaluations indicated safety at doses up 400 rats, without any noticeable physiological alterations. (30 mg/kg) administered orally h before gastric ulcer ameliorated stomach lesions lowered index area by 73.81% 77.87%, supplementation histopathological alterations restored barriers, including pH mucin secretion. Moreover, rhamnetin‐treated exhibited increased anti‐apoptotic heat shock protein 70 decreased Bax tissues. These findings line accumulated MDA, superoxide dismutase, catalase prostaglandin E2 levels, reduced serum inflammatory mediators (TNF‐α interleukin‐6) elevated interleukin‐10 cytokines. The outcomes indicate cicatrising against indomethacin‐mediated ulceration, possibly due modulatory actions on oxidative stress, inflammation apoptotic pathways.

Language: Английский

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Investigation of the Presence of Papillomavirus in Bovine Testicles and Determination of γH2AX and Cytochrome C in Testicular Tissues Determined to be Infected

Veterinary Medicine and Science, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 1, 2025

ABSTRACT This study aimed to detect the presence of bovine papillomavirus (BPV) in testicular tissue bulls over 1‐year old by immunohistochemical, immunofluorescence and molecular assay targeting methods. In addition, γH2AX cytochrome c expressions were evaluated immunohistochemical immunofluorescent methods samples positive for BPV agent. this study, 100 specimens that did not show any macroscopic papilloma findings collected. Testicular tissues collected examined using histologically molecularly present immunopositivity was detected 6 (6%) with IHC method 11 (11%) IF method. positivity four (4%) PCR determined be also showed seen two BPV‐positive method, observed agent Cytochrome found all When evaluated, cattle have papillomas revealed first time. It thought caused double‐stranded DNA breaks increased expression.

Language: Английский

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Effectiveness of Wharton’s jelly mesenchymal stem cell medium on burn wound healing: Focus on apoptosis, necrosis, and autophagy

Revista Científica de la Facultad de Ciencias Veterinarias, Journal Year: 2025, Volume and Issue: 35(1), P. 7 - 7

Published: March 3, 2025

The aim of this study is to evaluate the treatment efficacy Platelet–Rich Plasma (PRP), silver sulfadiazine, and Wharton Jelly Mesenchymal Stem Cell–Derived Conditioned Medium (WJ–MSC–CM) on burn wounds using a rat model. included four groups, each with 16 rats, groups were further divided into two subgroups (n=8) for 7th 14th days process. Group 1 received no after burn. 2 PRP (Platelet–Rich Plasma) first day 3 was treated sulfadiazine 4 WJ–MSC–CM In current study, expression Caspase–3, Bcl–2, TNF–α, p21, Beclin–1 genes among evaluated by Real–time PCR. exhibited lower Bcl–2 higher Caspase–3 compared other groups. TNF–α p21 high in control group showed findings demonstrate that exhibits healing comparable reference drug (silver sulfadiazine) inducing apoptosis autophagy reducing necroptosis DNA damage. Additionally, provided some positive benefits but less effective than treatments.

Language: Английский

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Determination by Immunohistochemistry of Acute Phase Proteins in Naturally Infected Sheep with Listeriosis

Acta veterinaria, Journal Year: 2025, Volume and Issue: 75(1), P. 25 - 37

Published: March 1, 2025

Abstract Listeriosis is an infectious and fatal disease affecting domestic mammals, poultry, humans worldwide. The effectiveness of local tissue expression acute phase proteins in listeriosis mammals not yet clear. aim this study to evaluate the 26 brainstem samples according distribution severity inflammation due natural sheep. material consisted paraffin blocks, including 20 from listeriosis-infected cases 6 healthy controls. Sections obtained blocks were subjected histopathological immunohistochemical analyses. Histopathological examination revealed normal histological structures brainstems control group. In contrast, sections exhibited findings such as micro abscesses composed neutrophil granulocytes microglial cells, gliosis, meningitis, congestion, perivascular cuffs, neuronal degeneration, neuronophagia. Based on inflammation, categorized into three groups: 5 mild (Group I), 8 moderate II), 7 severe III). Immunohistochemical analysis demonstrated significantly increased levels C-reactive protein (CRP), Haptoglobin (Hp), Serum Amyloid A (SAA) groups compared group, with highest statistical scores observed Group II III (p<0.001). suggest that acute-phase may play crucial roles pathophysiological processes naturally infected could express locally. Particularly, these progression provide valuable insights infection process.

Language: Английский

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Possible therapeutic effects of PROK2 in testicular ischemia/reperfusion injury: a preclinical study in Wistar albino rats

Tissue and Cell, Journal Year: 2025, Volume and Issue: 93, P. 102778 - 102778

Published: Feb. 8, 2025

Language: Английский

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Anchusa officinalis accelerates wound healing via the improvement of transforming growth factor beta 1 expressions, antioxidant levels, and inhibition of TNF-α, IL-6

Cutaneous and Ocular Toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 14

Published: March 26, 2025

Wound healing is an intricate, complicated process that needs special attention because of its related complications may occur if not treated properly or therapeutic insufficiency. Common bugloss (Anchusa officinalis L.) a deep-rooted, hairy perennial herb used in folk medicine for numerous human issues, including wound recovery. To delineate safety and potentials, we investigated the acute toxicity wound-healing effects Anchusa L. (APEAO) aerial part extracts on excisional neck injury rats. A uniform dorsal cut was formed twenty-four albino rats, which were arbitrarily divided into 4 groups daily with topical 0.2 ml dose following: group A, rats received 10% tween 20; B, intrasite gel; C D, had 250 500 mg/kg APEAO, respectively. The APEAO treatment did cause toxic damage administered up to 5 g/kg APEAO. In experiment, APEAO-treated skin exhibited significantly higher deposition tissue collagen fibroblast cells. contrast, inflammatory cells lower recovered tissues than positve control Topical application caused positive modulation Transforming Growth Factor Beta 1 (angiogenesis) skin, indicating elevated growth faster action. Moreover, significant elevation antioxidants (Superoxide dismutase, glutathione peroxidase, catalyze) hydroxyproline (collagen) content, lowering Malondialdehyde levels compared vehicle Serum chemicals (Transforming factor α, Interlukin-6, Interlukin-10) modulated following application. outcomes revealed regeneration potentials by modulatory actions several cellular processes, could serve as scientific evidence future investigation regarding production potent pharmaceuticals contraction.

Language: Английский

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Eucalyptol (1.8-cineole) attenuates gentamicin-induced liver injury

Etlik Veteriner Mikrobiyoloji Dergisi, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Gentamicin (GM), which is in the aminoglycoside antibiotic class, frequently preferred today treatment of diseases caused by gram-negative bacteria. However, its significant side effects on liver and kidney functions limit clinical usefulness. The antioxidant anti-inflammatory medical activities eucalyptol (EUC, 1.8-cineole) have been reported different studies. This study aimed to evaluate EUC GM-induced hepatotoxicity. groups are consisted control (C), EUC, GM + there were 7 rats each group. At end study, euthanized under appropriate conditions samples collected biochemical, histopathological immunohistochemical analyzes performed. It was determined that a important increase serum alanine aminotransferase (ALT), aspartate transferase (AST) gamma-glutamyl (GGT) enzymes group relative C (p

Language: Английский

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Evaluation of The Effects of Tarantula Cubensis Alcohol Extract and Sorafenib Treatments on P21 Protein, Total Antioxidant Capacity and Metabolic Profile in Experimental Rats Hepatocellular Carcinoma

Manas Journal of Agriculture Veterinary and Life Sciences, Journal Year: 2024, Volume and Issue: unknown, P. 201 - 209

Published: Oct. 22, 2024

Hepatocellular carcinoma (HCC) is defined as the sixth most common cancer type and third in terms of cancer-related deaths. Tarantula cubensis alcohol extract (TCAE, Theranekron) a homeopathic medicine frequently used veterinary treatment papilloma, mammary adenocarcinoma necrotic disorders. The present study aimed to reveal effectiveness TCAE Sorafenib (S) HCC induced by Diethylnitrosamine (DEN) N-nitrosomorpholine (NMOR)-induced rats. Rats were randomly divided into 7 groups: Control (C), + (CT), S (CS), Cancer (CC), CC+TCAE (CCT), CC+S (CCS), CC+TCAE+S (CCTS). In CC group, values for glucose, triglyceride (TG) total antioxidant capacity (TAC) found be significantly higher than all other groups (p&lt;0.001), while p21 levels lower (p&lt;0.05). It determined an increase serum (p&lt;0.05) significant decrease glucose TG (p&lt;0.001) CCT, CCS CCTS compared group. Histopathological examination revealed that group showed morphology, caused tumor incidence size. As result, it can said alone and/or combined with chemotherapy drugs antiproliferative effects on cells HCC. combination therapy may potentially synergize improve magnitude durability antitumor responses patients

Language: Английский

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