Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2023, Volume and Issue: 234, P. 115526 - 115526
Published: June 15, 2023
Language: Английский
Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2023, Volume and Issue: 234, P. 115526 - 115526
Published: June 15, 2023
Language: Английский
Critical Reviews in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 54(7), P. 2670 - 2687
Published: April 8, 2023
Efficient and sensitive detection of targets is one the motivations for constant development innovation various biosensors. CRISPR-Cas12a, a new generation gene editing tools, has shown excellent application potential in biosensor design construction. By combining with specific recognition element-aptamer, single-stranded oligonucleotide obtained by systematic evolution ligands exponential enrichment (SELEX) vitro screening, CRISPR-Cas12a also shows superior performance non-nucleic acid detection, such as small molecules, proteins, virus pathogenic bacteria. However, aptamer (CRISPR-Cas12a/Apt) still face some problems target single signal response mode narrow linear range. The diverse CRISPR-Cas12a/Apt biosensors necessary to meet needs environments. In this review, working principle was introduced recent progress summarized. Moreover, requirements critical parameters crRNA sequence, activator reaction system were discussed, which could provide reference efficient novel addition, challenges prospects CRISPR-Cas12a/Apt-based further presented.
Language: Английский
Citations
14Critical Reviews in Food Science and Nutrition, Journal Year: 2023, Volume and Issue: 64(18), P. 6395 - 6414
Published: Jan. 20, 2023
Food safety is a global issue in public hygiene. The accurate, sensitive, and on-site detection of various food contaminants performs significant implications. However, traditional methods suffer from the time-consuming professional operation, restricting their application. Hydrogels with merits highly porous structure, high biocompatibility, good shape-adaptability, stimuli-responsiveness offer promising biomaterial to design sensors for ensuring safety. This review describes emerging applications hydrogel-based inspection recent years. In particular, this study elaborates on fabrication strategies unique sensing mechanisms depending whether hydrogel stimuli-responsive or not. Stimuli-responsive hydrogels can be integrated functional ligands sensitive convenient via signal amplification transduction; while non-stimuli-responsive are mainly used as solid-state encapsulating carriers probe, nanomaterial, cell conductive media. addition, existing challenges, future perspectives, application prospects discussed. These practices greatly enrich scenarios improve performance detection.
Language: Английский
Citations
13Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: 152, P. 104681 - 104681
Published: Aug. 24, 2024
Language: Английский
Citations
5Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 375, P. 733 - 744
Published: Sept. 29, 2024
Language: Английский
Citations
5Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2023, Volume and Issue: 234, P. 115526 - 115526
Published: June 15, 2023
Language: Английский
Citations
12