EFSA Journal, Journal Year: 2023, Volume and Issue: 21(11)
Published: Nov. 1, 2023
This publication is linked to the following EFSA Supporting Publications articles: http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2023.EN-8441/full, http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2023.EN-8440/full, http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2023.EN-8437/full
Language: Английский
Archives of Toxicology, Journal Year: 2025, Volume and Issue: unknown
Published: April 28, 2025
Abstract Unraveling the associations between human exposure to environmental chemicals and potential neurotoxicity presents significant challenges. Evaluation of using animal testing is resource-intensive (financial, labor, use) faces uncertainties regarding biological relevance health outcomes. Therefore, there a need develop efficient human-relevant in vitro new approach methodologies (NAMs) screen evaluate for potential. Recording neural network activity microelectrode array (MEA) technology has been identified as reliable reproducible method evaluating neurotoxicity. Much this research performed 2D rodent-derived cell models. The ‘BrainSpheres MEA assay’ described study offers promising functional induced pluripotent stem (iPSC)-derived 3D brain model comprising neurons, astrocytes, oligodendrocytes. We demonstrate consistent spontaneous neuronal firing bursting parameters from 7-week-old BrainSpheres high-density technology. performance NAM was evaluated by conducting multi-concentration, 13 day with set ten chemicals. Neural metrics were assessed compared results 2D-MEA assay rodent cells. Loperamide domoic acid (two positive controls) demonstrated similar bioactivity profiles BrainSphere assay, while acetaminophen (assay negative control) inactive both assays. more potent 4/7 that active In future, reducing replicate variability larger will likely improve accuracy reliability assay. These preliminary findings suggest could be used alongside rat formation (rNFA) part tiered strategy, where hits rNFA are confirmed further characterized model, helping move toward animal-free toxicological testing.
Language: Английский
Citations
0
NAM journal., Journal Year: 2025, Volume and Issue: unknown, P. 100023 - 100023
Published: April 1, 2025
Language: Английский
Citations
0
Archives of Toxicology, Journal Year: 2025, Volume and Issue: unknown
Published: May 7, 2025
Language: Английский
Citations
0
Trends in Food Science & Technology, Journal Year: 2023, Volume and Issue: 142, P. 104191 - 104191
Published: Oct. 4, 2023
The conventional toxicological approach which primarily relies on animal testing has undergone several transformations in recent years due to limitations translational relevance, ethical and reproducibility concerns, high costs, time-consuming procedures. NAMs have emerged as alternative techniques that incorporate silico, chemico, vitro approaches provide mechanism-based insights with improved ethics human-relevant safety data. current landscape of employed for food risk assessments research regulatory settings is examined a focus their strengths, limitations, validation, standardisation, status. Furthermore, the challenges associated evaluation complex matrices are highlighted key considerations utilisation assessments. acceptance progressive majority demonstrating more relevance general chemical pharmaceutical cosmetics industries. There need advance approval address battery tests demonstrated applicable assessment matrices. review provides into status food-relevant identifies areas requiring adoption increase wider applicability.
Language: Английский
Citations
9
EFSA Journal, Journal Year: 2023, Volume and Issue: 21(11)
Published: Nov. 1, 2023
This publication is linked to the following EFSA Supporting Publications articles: http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2023.EN-8441/full, http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2023.EN-8440/full, http://onlinelibrary.wiley.com/doi/10.2903/sp.efsa.2023.EN-8437/full
Language: Английский
Citations
9