Exopolysaccharide production by salt-tolerant bacteria: Recent advances, current challenges, and future prospects

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130731 - 130731

Published: March 11, 2024

Language: Английский

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High-Level Production of l-Fucose by Plasmid-Free or Antibiotic-Independent Metabolically Engineered Escherichia coli Strains

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(17), P. 6619 - 6628

Published: April 17, 2024

l-Fucose is an important monosaccharide unit that exists in various biomasses, especially microalgae. Microbial l-fucose using metabolically engineered strains has attracted attention due to its high yield and industrial feasibility. Previously, we Escherichia coli MG1655 efficiently produce 2′-fucosyllactose by genomic integration. Herein, this plasmid-free strain was further integrating a specific α-l-fucosidase gene deleting the degradation pathway. Its effectiveness of biosynthesis inducer-free fermentation demonstrated both shake-flask fed-batch cultivation with titers 2.74 21.15 g/L, respectively. The precursor GDP-l-fucose supply strengthened obviously enhance introducing single plasmid expressing four pathway genes. hok/sok system introduced promote stabilization without antibiotic. final could antibiotics, 6.83 35.68 g/L fed-cultivation cultivation,

Language: Английский

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Optimization of l-Fucose Biosynthesis in Escherichia coli through Pathway Engineering and Mixed Carbon Source Strategy

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

This study presents an engineered strain of Escherichia coli specifically designed to enhance the production l-fucose while minimizing residues 2′-fucosyllactose. The optimization strategies employed include selection key enzymes, gene copy numbers, and fermentation using mixed carbon sources. metabolic flux was directed toward synthesis by integrating preferred 1,2-fucosyltransferase α-l-fucosidase into genome. Furthermore, numbers were optimized enzyme expression, thereby increasing production. Additionally, supply guanosine 5′-triphosphate improved, cofactors regenerated better regulate metabolism. Modifications transporter proteins effectively reduced accumulation implementation a glucose/glycerol co-fermentation strategy enhanced distribution efficiency. achieved yield 91.90 g/L in 5 L bioreactor, representing 80.01% increase over previous yields, with productivity 1.18 g L–1 h–1. is highest reported for l-fucose, demonstrating its potential industrial

Language: Английский

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Dual Antibiotic-Free Plasmid Systems Enable High-Efficiency l-Fucose Biosynthesis

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

l-Fucose, a functional monosaccharide with significant commercial potential in the pharmaceutical, nutraceutical, and cosmetic industries, faces challenges microbial production due to antibiotic-dependent plasmid maintenance systems. This study presents dual antibiotic-free strategy engineered Escherichia coli BL21(DE3) achieve high-efficiency l-fucose biosynthesis. By integration of hok/sok toxin-antitoxin system cysC-based auxotrophic selection into two plasmids, genetic stability retention were ensured without antibiotics. Metabolic pathway optimization involved enhancing GDP-l-fucose supply via promoter replacements, genomic key enzymes (α1,2-fucosyltransferase α-l-fucosidase), blocking degradation. The strain demonstrated robust performance, producing 7.99 g/L shake-flask fermentation 61.91 fed-batch cultivation─both antibiotic-free. titer represents highest reported yield date, highlighting effectiveness combining toxin–antitoxin systems for sustainable, high-productivity manufacturing.

Language: Английский

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Effects of Fucoidan and Fucoidan Oligosaccharides in Growth and Quorum Sensing Mediated Virulence Factor of Campylobacter Jejuni

Polysaccharides, Journal Year: 2025, Volume and Issue: 6(2), P. 24 - 24

Published: March 27, 2025

Fucoidan is a sulfated fucan marine polysaccharide with potential therapeutic applications, including antibacterial activity and the control of virulence factors associated quorum sensing. This study investigates bioactivity fucoidan derived from brown algae Ascophyllum nodosum, as well their oligosaccharides (OFuc; <3 kDa), on growth, motility, biofilm formation, adhesion Campylobacter jejuni, leading cause bacterial gastroenteritis worldwide. The results showed that decreased growth rate C. jejuni at concentrations greater than 25 µg/mL, while no effect was observed different (5–100 µg/mL) OFuc. Neither compound affected motility. Both OFuc inhibited abiotic formation diminished pathogen in concentration-dependent manner. also found recognized molecule through an enzyme-like lectin assay (ELLA) showing lectin-like adhesin-carbohydrate recognition. Overall, these suggest A. nodosum for controlling food industry, they open new avenues research into use aimed blocking infections caused by jejuni.

Language: Английский

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Development of a new L-fucose purity certified reference material

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2025, Volume and Issue: 263, P. 116922 - 116922

Published: May 2, 2025

Language: Английский

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Evaluation of potential prebiotic activity of a novel fucose-containing trisaccharide prepared from bacterial exopolysaccharides

Food Bioscience, Journal Year: 2024, Volume and Issue: 60, P. 104380 - 104380

Published: May 21, 2024

Language: Английский

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Molecular insights into FucR transcription factor to control the metabolism of L-fucose in Bifidobacterium longum subsp. infantis

Microbiological Research, Journal Year: 2024, Volume and Issue: 283, P. 127709 - 127709

Published: April 3, 2024

Language: Английский

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Generative adversarial network integrated with metabolomics identifies potential biomarkers related to quality changes of atemoya (Annona cherimola × Annona squamosa) stored at 10 and 25 °C

Food Chemistry, Journal Year: 2024, Volume and Issue: 470, P. 142679 - 142679

Published: Dec. 31, 2024

Language: Английский

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