Oncogene,
Journal Year:
2023,
Volume and Issue:
42(9), P. 665 - 678
Published: Jan. 3, 2023
Abstract
To
understand
how
malignant
tumors
develop,
we
tracked
cell
membrane,
nuclear
spindle,
and
cycle
dynamics
in
polyploid
giant
cancer
cells
(PGCCs)
during
the
formation
of
high-grade
serous
carcinoma
organoids
using
long-term
time-lapse
imaging.
Single
underwent
traditional
mitosis
to
generate
tissue
with
uniform
size,
while
others
formed
PGCCs
via
asymmetric
mitosis,
endoreplication,
multipolar
endomitosis,
fusion,
karyokinesis
without
cytokinesis.
restitution
fragmentation,
micronuclei
increase
contents
heterogeneity.
At
cellular
level,
development
was
associated
forming
transient
intracellular
cells,
termed
fecundity
cells.
The
can
be
decellularized
facilitate
fusion
synchronized
other
nuclei
for
subsequent
replication.
undergo
several
rounds
entosis
form
complex
structures,
structures.
multiple
modes
replication
absence
cytokinesis
leads
an
nuclear-to-cytoplasmic
(N/C)
ratio
reproduction,
which
is
remarkably
similar
mode
division
pre-embryogenesis.
Our
data
support
that
may
represent
a
central
regulator
histogenesis,
intratumoral
heterogeneity,
immune
escape,
macroevolution
de-repression
suppressed
pre-embryogenic
program
somatic
Nature Communications,
Journal Year:
2016,
Volume and Issue:
7(1)
Published: April 22, 2016
The
mammary
gland
represents
a
unique
tissue
to
study
organogenesis
as
it
predominantly
develops
in
the
post-natal
animal
and
undergoes
dramatic
morphogenetic
changes
during
puberty
reproductive
cycle.
physiological
function
of
is
produce
milk
sustain
newborn.
Here
we
view
lactating
through
three-dimensional
confocal
imaging
intact
tissue.
We
observed
that
majority
secretory
alveolar
cells
are
binucleated.
These
first
arise
very
late
pregnancy
due
failure
cytokinesis
larger
than
mononucleated
cells.
Augmented
expression
Aurora
kinase-A
Polo-like
kinase-1
at
lactogenic
switch
likely
mediates
formation
binucleated
Our
findings
demonstrate
an
important
role
for
polyploid
epithelial
lactation,
based
on
their
presence
five
different
species,
suggest
evolved
maximize
production
promote
survival
offspring
across
all
mammalian
species.
Proceedings of the National Academy of Sciences,
Journal Year:
2021,
Volume and Issue:
118(7)
Published: Jan. 27, 2021
We
present
a
unifying
theory
to
explain
cancer
recurrence,
therapeutic
resistance,
and
lethality.
The
basis
of
this
is
the
formation
simultaneously
polyploid
aneuploid
cells,
polyaneuploid
cells
(PACCs),
that
avoid
toxic
effects
systemic
therapy
by
entering
state
cell
cycle
arrest.
independent
which
classically
associated
oncogenic
mutations
have
already
occurred.
PACCs
been
generally
disregarded
as
senescent
or
dying
cells.
Our
states
resistance
driven
PACC
enabled
accessing
program
allows
an
double
its
genomic
content,
followed
entry
into
nondividing
protect
DNA
integrity
ensure
survival.
Upon
removal
stress,
e.g.,
chemotherapy,
undergo
depolyploidization
generate
resistant
progeny
make
up
bulk
within
tumor.
Oncogene,
Journal Year:
2023,
Volume and Issue:
42(9), P. 665 - 678
Published: Jan. 3, 2023
Abstract
To
understand
how
malignant
tumors
develop,
we
tracked
cell
membrane,
nuclear
spindle,
and
cycle
dynamics
in
polyploid
giant
cancer
cells
(PGCCs)
during
the
formation
of
high-grade
serous
carcinoma
organoids
using
long-term
time-lapse
imaging.
Single
underwent
traditional
mitosis
to
generate
tissue
with
uniform
size,
while
others
formed
PGCCs
via
asymmetric
mitosis,
endoreplication,
multipolar
endomitosis,
fusion,
karyokinesis
without
cytokinesis.
restitution
fragmentation,
micronuclei
increase
contents
heterogeneity.
At
cellular
level,
development
was
associated
forming
transient
intracellular
cells,
termed
fecundity
cells.
The
can
be
decellularized
facilitate
fusion
synchronized
other
nuclei
for
subsequent
replication.
undergo
several
rounds
entosis
form
complex
structures,
structures.
multiple
modes
replication
absence
cytokinesis
leads
an
nuclear-to-cytoplasmic
(N/C)
ratio
reproduction,
which
is
remarkably
similar
mode
division
pre-embryogenesis.
Our
data
support
that
may
represent
a
central
regulator
histogenesis,
intratumoral
heterogeneity,
immune
escape,
macroevolution
de-repression
suppressed
pre-embryogenic
program
somatic
