Phylogenetic and taxonomic insights into Betula: low-coverage whole genome sequencing and plastome analysis with focus on the rare Ukrainian endemic species Betula klokovii Zaverucha

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Abstract Betula klokovii Zaverucha is a rare endemic species of Ukraine that still not well taxonomically studied. In the current pilot study, we performed low-coverage whole genome sequencing for B. , related ( pendula Roth and pubescens Ehrh.) assumed hybrid × pendula, assessed genomic structure taxa with different mapping settings using UMAP non-linear dimension reduction algorithm, extracted assembled plastomes. Single Nucleotide Polymorphism (SNP) analysis based on (LC-WGS) followed by visualization reveals separation from other analysed taxa. The best taxonomic resolution was achieved reads filtered contamination. contrast, result in obtaining complete plastome assemblies via NOVOPlasty pipeline raw reads. size eight newly plastid genomes ranges between 160,535 160,625bp, GC content 36,1%. We annotated 130 genes (113 unique) all assemblies. addition, investigated klokovii’s relationships 20 birch two intraspecific reconstructing plastome-based Bayesian inference maximum likelihood phylogenies. Overall, phylogeny provides better comparison to phylogenies few or nuclear molecular markers. However, it could be affected chloroplast capture, some factors like quality assembly, suitable detect hybrids when used alone. particular, found likely separate taxon closely but morphologically genetically distinct. study shows genome-wide SNP data have certain potential addressing issues specific within genus L. fully leverage this approach, suggest collecting much larger number sequences sequenced assembled. For understanding there need reference-grade chromosome scale polyploid species.

Language: Английский

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Gender-Responsive Implementation of Biodiversity and Nature Conservation Treaties

Cambridge University Press eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 126 - 144

Published: Feb. 13, 2025

Language: Английский

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A chromosome-level genome assembly of the European green toad (Bufotes viridis)

G3 Genes Genomes Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Abstract The European green toad (Bufotes viridis) is geographically widely distributed. While the species global conservation status labeled as of least concern by IUCN, it declining in many parts its range where populations are fragmented and isolated. A high-quality reference genome an important resource for genomic researchers who trying to understand interpret signals population decline, inbreeding, accumulation deleterious mutations. Here, we assembled annotated a chromosome-level B. viridis part Reference Genome Atlas pilot project. assembly, with size ∼3.89 Gb consists 11 chromosomes additional 2,096 unplaced scaffolds. final assembly had scaffold N50 value 478.39 Mb covered 90.4% single copy tetrapod orthologs, 46.7% repetitive elements. Finally, total 23,830 protein-coding genes matching known gene, together 56,974 mRNAs were predicted. This will benefit amphibian evolutionary genomics research enable genetic studies inform practical work on this species.

Language: Английский

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Genomic Insights Into the Origin, Decline and Recovery of the Once Critically Endangered Iberian Lynx

Molecular Ecology, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

ABSTRACT The Iberian lynx was at the brink of extinction by year 2000 but has since then, and thanks to intensive conservation measures, gone through a remarkable recovery, providing much‐welcomed encouraging success story. Genetic issues have probably contributed decline in past, genetic management inbreeding diversity is likely contributing its recent recovery. species an early adopter genomic approaches, combination extreme decline, monitoring programme extensive resources data makes excellent model for genomics. Here, we review how knowledge evolutionary demographic history, evaluation status time, including historical ancient data, this information prompted guided actions. In process, genomics provided valuable insights into dynamics functional variation bottlenecked populations consequences intraspecific interspecific admixtures. more applied terms, subjected ambitious programme, covering captive, remnant reintroduced populations, which succeeded improving thereby Current work aims expanding these contributions with novel while capitalising on genealogical ongoing non‐invasive programme.

Language: Английский

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Essential skills for the training of conservation social scientists

Conservation Biology, Journal Year: 2025, Volume and Issue: 39(2)

Published: April 1, 2025

Abstract Since 2000, the field of biodiversity conservation has been reckoning with historical lack effective engagement social sciences in parallel rapid declines and escalating concerns regarding socioecological justice exacerbated by many common practices. As a result, there is now wide recognition among scholars practitioners importance understanding engaging human dimensions practice. Developing applying theoretical practical knowledge related to sciences, therefore, should be priority for people working conservation. We considered training needs next generation science professionals surveying multiple sectors. Based on 119 responses, 3 most cited soft skills (i.e., nontechnical abilities that facilitate interpersonal interaction, collaboration, adaptability diverse contexts) were cultural awareness ability understand values perspectives others, management conflict resolution skills, develop maintain inter‐ intraorganizational networks relationships. The technical expertise behavior change expertise, government policy, general critical thinking problem‐solving skills. Overall, we found current scientists believe students early career conservationists prioritize rather than effective. These also correlated hardest acquire through on‐the‐job training. suggest essential including awareness, networking, thinking, statistical analysis tailored sectoral regional needs.

Language: Английский

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