Foods, Journal Year: 2024, Volume and Issue: 13(11), P. 1782 - 1782
Published: June 6, 2024
The fermentation process of Chinese Baijiu’s fermented grains involves the intricate succession and metabolism microbial communities, collectively shaping quality. Understanding composition these living communities within is crucial for comprehending flavor formation mechanisms. However, conducting high-throughput analysis complex system poses significant challenges. Thus, this study addressed challenge by devising a framework using light-flavor Baijiu as model. This combined propidium monoazide (PMA) pretreatment technology with amplicon sequencing techniques. Optimal PMA treatment parameters, including concentration 50 μM incubation in darkness 5 min followed an exposure period min, were identified. Utilizing protocol, viable microorganism biomass ranging from 8.71 × 106 to 1.47 108 copies/μL was successfully detected grain samples. Subsequent revealed distinct community structures between untreated PMA-treated groups, notable differences relative abundance compositions, particularly dominant species such Lactobacillus, Bacillus, Pediococcus, Saccharomycopsis, Issatchenkia Pichia, identified LEfSe analysis. results confirmed efficacy PMA-amplicon analyzing furnished methodological investigating diverse traditional foods. technical holds considerable significance advancing our understanding mechanisms intrinsic
Language: Английский
Citations
1Rational probe design for efficient rRNA depletion and improved metatranscriptomic analysis of human microbiomes
BMC Microbiology, Journal Year: 2023, Volume and Issue: 23(1)
Published: Oct. 20, 2023
The microbiota that colonize the human gut and other tissues are dynamic, varying both in composition functional state between individuals over time. Gene expression measurements can provide insights into microbiome function. However, efficient unbiased removal of microbial ribosomal RNA (rRNA) presents a barrier to acquiring metatranscriptomic data. Here we describe probe set achieves enzymatic rRNA complex human-associated communities. We demonstrate custom be further refined through an iterative design process efficiently deplete from range samples. Using synthetic nucleic acid spike-ins, show depletion does not introduce substantial quantitative error gene profiles. Successful allows for characterization taxonomic profiles, including during development microbiome. pan-human probes described here powerful tool studying transcriptional dynamics function
Language: Английский
Citations
3Host species drive composition of mosquito virome
Nature Ecology & Evolution, Journal Year: 2024, Volume and Issue: 8(5), P. 852 - 853
Published: March 22, 2024
Language: Английский
Citations
0A multi-omics meta-analysis of rhizosphere microbiome reveals growth-promoting marker bacteria at different stages of legume development
Authorea (Authorea), Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 1, 2024
Plant-microbe interactions have been studied extensively in legumes, but the influence of host developmental stages on its microbiome remains poorly understood. The rhizospheric region enriched with microbial diversity presents an optimal environment to investigate this relationship. We employed a multi-omics meta-analysis approach identify bacteria co-existing legumes at different stages. data from eight legume species across various geographical locations, soil conditions and (vegetative, reproductive maturation) were included study. A total 10 marker identified vegetative, maturation stages, most them known for their role plant growth promotion. functional profiling further elucidated expression these genes, indicating active presence bacteria. Co-expression network analysis revealed involvement gene clusters biological processes such as cobalt nitrogen metabolism. Furthermore, pathway enrichment illustrated metabolic pathways biosynthesis secondary metabolites, biotin metabolism carbon fixation photosynthetic organisms. Our study positive relationship between plant, suggesting crucial development that could aid advancement strategies improve crop yield.
Language: Английский
Citations
0Techniques for Studying the Microbiome
Published: Jan. 1, 2024
Language: Английский
Citations
0