Identification of sex-biased and gonadal developmental miRNAs during critical windows of early gonadal differentiation in the Chinese giant salamander (Andrias davidianus)

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 12, 2025

The Chinese giant salamander (CGS), Andrias davidianus, the largest living amphibian, is of significant conservation importance for its wild populations and serves as a valuable economic species in aquaculture. Given that male CGS are larger than female, understanding mechanisms behind gonadal differentiation development critical optimizing production This study conducted small RNA-seq on female gonads during two key windows CGS. PCA analysis revealed distinct clustering within groups clear between groups. A total 374 miRNAs were identified, including 162 known 212 novel miRNAs. Differential expression enrichment across time points identified nine sex-biased involved differentiation, let-7b-3p, miR-3529-3p, miR-34c-5p, miR-10-5p, miR-7-5p, four miRNAs, network these with their target genes constructed. Furthermore, our findings suggest processes likely regulated by provides comprehensive related CGS, offering theoretical foundation enhancing aquaculture productivity through targeted sex ratio management.

Language: Английский

Maternal inheritance of primary sex ratios in the dark-winged fungus gnatLycoriella ingenua

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Abstract Sex determination mechanisms in insects are extraordinarily diverse, although most species have zygotic genotypic sex where is established by chromosomes upon fertilisation. Dark-winged fungus gnats (Diptera: Sciaridae) a large and speciose family of flies result an unusual interplay zygotic, maternal, environmental factors. This causes some to produce clutches offspring that deviate considerably from the standard 1:1 Fisherian ratio. An early study suggested these primary ratios may be heritable mother daughter, but this observation has not been corroborated genetic basis for trait remains unknown. Other studies found species, there additional temperature effect on ratio, again mechanism Here, we perform sibling crosses temperature-shift experiments recently isolated line Lycoriella ingenua find evidence highly variable ratios, no significant effect. We discuss consequences our findings understanding evolution more broadly clade.

Language: Английский