The Role of Capsid in the Early Steps of HIV-1 Infection: New Insights into the Core of the Matter DOI Creative Commons

Nawal AlBurtamani,

Alwin Paul,

Ariberto Fassati

et al.

Viruses, Journal Year: 2021, Volume and Issue: 13(6), P. 1161 - 1161

Published: June 17, 2021

In recent years, major advances in research and experimental approaches have significantly increased our knowledge on the role of HIV-1 capsid virus life cycle, from reverse transcription to integration gene expression. This makes protein a good pharmacological target inhibit replication. review covers current understanding viral cycle its interaction with different host factors that enable transcription, trafficking towards nucleus, nuclear import into chromosomes. It also describes promising small molecules, some them clinical trials, as potential targets for therapy.

Language: Английский

Induced pluripotent stem cell-derived engineered T cells, natural killer cells, macrophages, and dendritic cells in immunotherapy DOI

Dixuan Xue,

Shan Lu, Hailing Zhang

et al.

Trends in biotechnology, Journal Year: 2023, Volume and Issue: 41(7), P. 907 - 922

Published: Feb. 28, 2023

Language: Английский

Citations

45
Sensor Sensibility—HIV-1 and the Innate Immune Response DOI Creative Commons
Xin Yin, Simon Langer, Zeli Zhang

et al.

Cells, Journal Year: 2020, Volume and Issue: 9(1), P. 254 - 254

Published: Jan. 20, 2020

Innate immunity represents the human immune system's first line of defense against a pathogenic intruder and is initiated by recognition conserved molecular structures known as pathogen-associated patterns (PAMPs) specialized cellular sensors, called pattern receptors (PRRs). Human immunodeficiency virus type 1 (HIV-1) unique RNA that causes acquired syndrome (AIDS) in infected individuals. During replication cycle, HIV-1 undergoes reverse transcription its genome integrates resulting DNA into genome. Subsequently, integrated provirus results production new virions spreading infection virus. Throughout viral numerous nucleic acid derived PAMPs can be recognized diverse set innate sensors cells. However, has evolved efficient strategies to evade or counteract this surveillance downstream responses. Understanding underpinnings concerted actions system, well corresponding evasion mechanisms during infection, critical understanding transmission pathogenesis, may provide important guidance for design appropriate adjuvant vaccine strategies. Here, we summarize current knowledge basis sensing cells, including CD4+ T dendritic macrophages. Furthermore, discuss underlying which regulated, describe developed

Language: Английский

Citations

70
Factors Regulating the Activity of LINE1 Retrotransposons DOI Open Access
Maria Protasova, Т. В. Андреева, Е. И. Рогаев

et al.

Genes, Journal Year: 2021, Volume and Issue: 12(10), P. 1562 - 1562

Published: Sept. 30, 2021

LINE-1 (L1) is a class of autonomous mobile genetic elements that form somatic mosaicisms in various tissues the organism. The activity L1 retrotransposons strictly controlled by many factors and germ cells at all stages ontogenesis. Alteration was noted number diseases: neuropsychiatric autoimmune diseases, as well forms cancer. Altered for some pathologies associated with epigenetic changes defects genes involved their repression. This review discusses molecular mechanisms retrotransposition regulation elements. contribution controlling expression distribution genome occurs retrotransposition. transcriptional, post-transcriptional integration into described detail. Finally, this also focuses on evolutionary aspects accumulation interplay host system.

Language: Английский

Citations

35
De-ubiquitination of SAMHD1 by USP7 promotes DNA damage repair to overcome oncogenic stress and affect chemotherapy sensitivity DOI Creative Commons
Jingwei Liu, Tingting Zhou, Xiang Da Dong

et al.

Oncogene, Journal Year: 2023, Volume and Issue: 42(22), P. 1843 - 1856

Published: April 20, 2023

Abstract Oncogenic stress induces DNA damage repair (DDR) that permits escape from mitotic catastrophe and allows early precursor lesions during the evolution of cancer. SAMHD1, a dNTPase protecting cells viral infections, has been recently found to participate in process. However, its role tumorigenesis remains largely unknown. Here, we show SAMHD1 is up-regulated early-stage human carcinoma tissues cell lines under oxidative or genotoxic insults. We further demonstrate de-ubiquitinating enzyme USP7 interacts with de-ubiquitinates it at lysine 421, thus stabilizing protein expression for interaction CtIP DDR, which promotes tumor survival stress. Furthermore, levels positively correlates various carcinomas, associated an unfavorable outcome patients who underwent chemotherapy. Moreover, inhibitor sensitizes chemotherapeutic agents by decreasing vitro vivo. These findings suggest de-ubiquitination DDR overcome oncogenic affect chemotherapy sensitivity.

Language: Английский

Citations

16
A Spotlight on Viruses—Application of Click Chemistry to Visualize Virus-Cell Interactions DOI Creative Commons
Thorsten G. Müller, Volkan Sakin, Bárbara Müller

et al.

Molecules, Journal Year: 2019, Volume and Issue: 24(3), P. 481 - 481

Published: Jan. 29, 2019

The replication of a virus within its host cell involves numerous interactions between viral and cellular factors, which have to be tightly controlled in space time. intricate interplay exploitation pathways the intrinsic defense mechanisms is difficult unravel by traditional bulk approaches. In recent years, novel fluorescence microscopy techniques single tracking transformed investigation dynamic virus-host interactions. A prerequisite for application these imaging-based methods attachment fluorescent label structure interest. However, their small size, limited coding capacity multifunctional proteins render viruses particularly challenging targets labeling Click chemistry conjunction with genetic code expansion provides virologists toolbox site-specific, minimally invasive virion components, whose potential has just recently begun exploited. Here, we summarize achievements, current developments future challenges nucleic acids, proteins, glycoproteins or lipids using click order study processes virus-cell

Language: Английский

Citations

38
From Entry to Egress: Strategic Exploitation of the Cellular Processes by HIV-1 DOI Creative Commons

Pavitra Ramdas,

Amit Kumar Sahu, Tarun Mishra

et al.

Frontiers in Microbiology, Journal Year: 2020, Volume and Issue: 11

Published: Dec. 4, 2020

HIV-1 employs a rich arsenal of viral factors throughout its life cycle and co-opts intracellular trafficking pathways. This exquisitely coordinated process requires precise manipulation the host microenvironment, most often within defined subcellular compartments. The virus capitalizes on by modulating cell-surface proteins cleverly exploiting nuclear import pathways for post entry events, among other key processes. Successful virus-cell interactions are indeed crucial in determining extent infection. By evolving defenses against restriction factors, while simultaneously dependency presents fascinating montage an ongoing host-virus arms race. Herein, we provide overview how exploits native functions cell discuss recent findings that fundamentally change our understanding post-entry replication events.

Language: Английский

Citations

38
New Insights on Molecular Mechanism of Hepatitis B Virus Covalently Closed Circular DNA Formation DOI Creative Commons
Alexander Marchetti, Haitao Guo

Cells, Journal Year: 2020, Volume and Issue: 9(11), P. 2430 - 2430

Published: Nov. 6, 2020

The chronic factor of the Hepatitis B Virus (HBV), specifically covalently closed circular DNA (cccDNA), is a highly stable and active viral episomal genome established in livers hepatitis patients as constant source disease. Being able to target eliminate cccDNA end goal for genuine cure HBV. Yet how HBV formed from genomic relaxed (rcDNA) by what host factors had been long-standing research questions. It generally acknowledged that hijacks cellular functions turn open conformation rcDNA into through repair mechanisms. With great efforts community, there have several recent leaps our understanding formation. this review analyze reports showing evidence factor's involvement molecular pathway biosynthesis.

Language: Английский

Citations

36
SAMHD1 dysfunction induces IL-34 expression via NF-κB p65 in neuronal SH-SY5Y cells DOI
Ling Zhang, Wenjing Wang, Ting Chen

et al.

Molecular Immunology, Journal Year: 2024, Volume and Issue: 168, P. 1 - 9

Published: Feb. 17, 2024

Language: Английский

Citations

4
CD81 association with SAMHD1 enhances HIV-1 reverse transcription by increasing dNTP levels DOI
Vera Rocha‐Perugini,

Henar Suárez,

Susana Álvarez

et al.

Nature Microbiology, Journal Year: 2017, Volume and Issue: 2(11), P. 1513 - 1522

Published: Sept. 1, 2017

Language: Английский

Citations

37
SAMHD1 Regulates Human Papillomavirus 16-Induced Cell Proliferation and Viral Replication during Differentiation of Keratinocytes DOI Creative Commons
Claire D. James, Apurva T. Prabhakar,

Raymonde Otoa

et al.

mSphere, Journal Year: 2019, Volume and Issue: 4(4)

Published: Aug. 6, 2019

HPVs are causative agents in human cancers and responsible for around of 5% all cancers. A better understanding the viral life cycle keratinocytes will facilitate development novel therapeutics to combat HPV-positive Here, we present a unique keratinocyte model identify host proteins that specifically interact with HPV16. Using this system, report cellular gene, SAMHD1, is regulated by HPV16 at RNA protein levels keratinocytes. Elimination SAMHD1 from these cells using CRISPR/Cas9 editing promotes enhanced proliferation elevated replication but not do have Our study demonstrates specific intricate interplay between during establishes system assist exploring factors critical HPV pathogenesis.

Language: Английский

Citations

33