Gut-on-Chip microphysiological systems: Latest advances in the integration of sensing strategies and adoption of mature detection mechanisms DOI Creative Commons
M.A. Signore, Chiara De Pascali, Lucia Giampetruzzi

et al.

Sensing and Bio-Sensing Research, Journal Year: 2021, Volume and Issue: 33, P. 100443 - 100443

Published: July 21, 2021

Synergic efforts in microfabrication processes, cells culture and tissue engineering promoted extraordinary progress Organ-on-Chip (OoC) technology, leading to the development of vitro microphysiological models able recapitulate microenvironment key biochemical, functional, structural mechanical features specific tissues living organs. In order assess functionality these cell cultures with every increasing biological complexity, it is also important equip OoCs miniaturized sensing devices monitor physical chemical parameters related pathophysiological cell-cell interactions. Gut one most interesting studied human organs: performs multiple fundamental body functions, from transport, absorption metabolism nutrients drugs, maturation immune system host protection pathogens infections. this Review, an overview Gut-on-Chip (GoC) systems provided, a special attention focused on relevant strategies integrated into GoC, aimed at monitoring situ intestine functionalities. Advantages limitations associated currently physical, chemical, biochemical sensors are discussed, together challenges that technology still faces, possible adaptive solutions coming other developed OoC models. Finally, we focus how gut microbiota connect organs role understanding progression many diseases, such as recent pandemic infection caused by SARS-CoV-2 virus.

Language: Английский

Organ-on-a-chip technology for nanoparticle research DOI Creative Commons

Shawn Kang,

Sunghee Estelle Park, Dongeun Huh

et al.

Nano Convergence, Journal Year: 2021, Volume and Issue: 8(1)

Published: July 8, 2021

The last two decades have witnessed explosive growth in the field of nanoengineering and nanomedicine. In particular, engineered nanoparticles garnered great attention due to their potential enable new capabilities such as controlled targeted drug delivery for treatment various diseases. With rapid progress nanoparticle research, increasing efforts are being made develop technologies vitro modeling analysis efficacy safety nanotherapeutics human physiological systems. Organ-on-a-chip technology represents most recent advance this effort that provides a promising approach address limitations conventional preclinical models. paper, we present concise review studies demonstrating how emerging can be applied nanoparticles. specific focus is examine use organ-on-a-chip models toxicity assessment used therapeutic applications. We also discuss challenges future opportunities implementing research.

Language: Английский

Citations

65
Recent advances in lung-on-a-chip models DOI
Isabella Francis, Jesus Shrestha, Keshav Raj Paudel

et al.

Drug Discovery Today, Journal Year: 2022, Volume and Issue: 27(9), P. 2593 - 2602

Published: June 18, 2022

Language: Английский

Citations

63
Organoid, organ-on-a-chip and traditional Chinese medicine DOI Creative Commons
Jiayue Yang, Yu Jiang, Mingxing Li

et al.

Chinese Medicine, Journal Year: 2025, Volume and Issue: 20(1)

Published: Feb. 12, 2025

Abstract In the past few years, emergence of organoids and organ-on-a-chip (OOAC) technologies, which are complementary to animal models two-dimensional cell culture methods can better simulate internal environment human body, provides a new platform for traditional Chinese medicine (TCM) studies. Organoids OOAC techniques have been increasingly applied in fields drug screening, assessment development, personalized therapies, developmental biology, there some application cases TCM this review, we summarized current status using organoid technologies research provide key insights future study. It is believed that will play more important roles make greater contributions innovative development TCM.

Language: Английский

Citations

1
Organoids and organ chips in ophthalmology DOI Creative Commons
Navid Manafi, Fereshteh Shokri, Kevin Achberger

et al.

The Ocular Surface, Journal Year: 2020, Volume and Issue: 19, P. 1 - 15

Published: Nov. 19, 2020

Recent advances have driven the development of stem cell-derived, self-organizing, three-dimensional miniature organs, termed organoids, which mimic different eye tissues including retina, cornea, and lens. Organoids engineered microfluidic organ-on-chips (organ chips) are transformative technologies that show promise in simulating architectural functional complexity native organs. Accordingly, they enable exploration facets human disease not accurately recapitulated by animal models. Together, these will increase our understanding basic physiology structures, us to interrogate unknown aspects ophthalmic pathogenesis, serve as clinically-relevant surrogates for evaluation ocular therapeutics. Both burden prevalence monogenic multifactorial diseases, can cause visual impairment or blindness, population warrants a paradigm shift towards organoids organ chips provide sensitive, quantitative, scalable phenotypic assays. In this article, we review current situation ophthalmology discuss how be leveraged translational applications.

Language: Английский

Citations

65
The future of phenotypic drug discovery DOI Creative Commons
Ellen L. Berg

Cell chemical biology, Journal Year: 2021, Volume and Issue: 28(3), P. 424 - 430

Published: Feb. 1, 2021

Language: Английский

Citations

42
Biomimetic Alveolus-on-a-Chip for SARS-CoV-2 Infection Recapitulation DOI Creative Commons
Ting Cao, Changmin Shao, Xiaoyu Yu

et al.

Research, Journal Year: 2022, Volume and Issue: 2022

Published: Jan. 1, 2022

SARS-CoV-2 has caused a severe pneumonia pandemic worldwide with high morbidity and mortality. How to develop preclinical model for recapitulating pathogenesis is still urgent essential the control of pandemic. Here, we have established 3D biomimetic alveolus-on-a-chip mechanical strain extracellular matrix taken into consideration. We validated that capable key physiological characteristics human alveolar units, which lays fundamental basis viral infection studies at organ level. Using virus-analogous chemicals pseudovirus, explored virus blocking ability antibodies during infection. This work provides favorable platform SARS-CoV-2-related researches great potential physiology pathophysiology lung level in vitro.

Language: Английский

Citations

37
Gut-on-chip for ecological and causal human gut microbiome research DOI
Shirin Moossavi, Marie‐Claire Arrieta, Amir Sanati‐Nezhad

et al.

Trends in Microbiology, Journal Year: 2022, Volume and Issue: 30(8), P. 710 - 721

Published: Feb. 18, 2022

Language: Английский

Citations

34
Organ-on-a-chip microengineering for bio-mimicking disease models and revolutionizing drug discovery DOI Creative Commons
Tanvir Ahmed

Biosensors and Bioelectronics X, Journal Year: 2022, Volume and Issue: 11, P. 100194 - 100194

Published: July 15, 2022

The core of the drug research and screening processes is predicting effect drugs prior to human clinical trials. Due 2D cell culture animal models' poor predictability, cost discovery continuously rising. development organ-on-a-chip technology, an alternative traditional preclinical testing models, resulted from intersection microfabrication & tissue engineering. Preclinical safety effectiveness improved by ability technologies mimic important physiological functions necessary for understanding effects. Organ-on-a-chip could drastically improve success rate thereby better how will act on a term used describe microengineered biomimetic device that mimics structure functionality tissue. It integrates engineering, biology, biomaterial miniature platform. To reflect physiology in vitro bridge gap between vivo data, simplification shouldn't compromise relevance. At this level technological development, biomedical engineers specializing engineering are more than ever expedite transfer technology academic lab bench specialized product institutions ever-growing market. This review focuses recent advancements discusses potential based current available literature.

Language: Английский

Citations

30
Advances of microfluidic lung chips for assessing atmospheric pollutants exposure DOI Creative Commons
Hui Wang,

Fangchao Yin,

Zhongyu Li

et al.

Environment International, Journal Year: 2023, Volume and Issue: 172, P. 107801 - 107801

Published: Feb. 1, 2023

Atmospheric pollutants, including particulate matters, nanoparticles, bioaerosols, and some chemicals, have posed serious threats to the environment human's health. The lungs are responsible organs for providing interface between circulatory system external environment, where pollutant particles can deposit or penetrate into bloodstream circulation. Conventional studies decipher mechanism underlying air pollution human health quite limited, due lack of reliable models that reproduce in vivo features lung tissues after pollutants exposure. In past decade, advanced near-to-native chips, combining cell biology with bioengineered technology, present a new strategy atmospheric assessment narrow gap 2D culture animal models. this review, key artificial chips cutting-edge technologies chip manufacture introduced. recent progresses exposure summarized highlighted. We further discuss current challenges future opportunities development their potential utilities associated toxicity testing drug screening.

Language: Английский

Citations

21
Recent advances in liver‐on‐chips: Design, fabrication, and applications DOI Creative Commons
Linjie Qiu, Bin Kong, Tiantian Kong

et al.

Smart Medicine, Journal Year: 2023, Volume and Issue: 2(1)

Published: Feb. 1, 2023

Abstract The liver is a multifunctional organ and the metabolic center of human body. Most drugs toxins are metabolized in liver, resulting varying degrees hepatotoxicity. damage will seriously affect health, so it very important to study prevention treatment diseases. At present, there many research studies this field. However, most them based on animal models, which limited by time‐consuming processes species difference between animals. In recent years, liver‐on‐chips have emerged developed rapidly expected replace models. Liver‐on‐chips refer use small number cells chips simulate microenvironment ultrastructure vivo. They hold extensive applications multiple fields reproducing unique physiological functions vitro. review, we first introduced physiology pathology then described cell system liver‐on‐chips, chip‐based transplantation, drug screening, evaluation. Finally, discussed currently encountered challenges future trends liver‐on‐chips.

Language: Английский

Citations

18