Development Growth & Differentiation,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 21, 2025
Abstract
In
previous
studies,
we
have
established
approximately
15
cultured
cell‐lines
derived
from
planula
larvae
of
Acropora
tenuis
.
Based
on
their
morphology
and
behavior,
these
cells
were
classified
into
three
types,
flattened
amorphous
(FAmCs),
vacuolated
adherent
(VAdCs),
small
smooth
(SSmCs).
FAmCs
include
fibroblast‐like
spherical,
brilliant
brown
(BBrCs),
which
are
transformable
to
each
other.
To
examine
the
larval
origin
cell
raised
antibodies:
anti‐AtMLRP2
that
appears
recognize
FAmC,
anti‐AtAHNAK
for
BBrC,
anti‐AtSOMP5
anti‐AtEndoG
SSmC,
anti‐AtGal
anti‐AtFat4
VAdC,
respectively.
Anti‐AtMLRP2
antibody
stained
in
vivo
stomodeum
neuroblast‐like
embedded
ectoderm
around
aboral
pole.
Anti‐AtAHNAK
neuron‐like
cells,
both
also
with
neuron‐specific
tubulin
β‐3
antibody.
These
results
suggest
vitro
BBrCs
share
neuronal
properties
common.
Two
antibodies
SSmCs,
anti‐AtEndoG,
suggesting
SSmCs
properties.
VAdCs,
anti‐AtFat4,
endoderm
VAdCs
Therefore,
lines
appear
retain
stomodeum,
neuroblast,
ectoderm,
or
endoderm.
Each
them
may
be
used
future
investigations
reveal
cellular
molecular
types
coral
larvae,
such
as
potential
symbiosis.
Biology Letters,
Journal Year:
2022,
Volume and Issue:
18(11)
Published: Nov. 1, 2022
Scleractinian
corals
are
essential
ecosystem
engineers,
forming
the
basis
of
coral
reef
ecosystems.
However,
these
organisms
in
decline
globally,
part
due
to
rising
disease
prevalence.
Most
dependent
on
symbiotic
interactions
with
single-celled
algae
from
family
Symbiodiniaceae
meet
their
nutritional
needs,
however,
suppression
host
immunity
may
be
this
relationship.
To
explore
immunological
consequences
algal
symbioses
scleractinian
corals,
we
investigated
constitutive
immune
activity
facultatively
coral,
Astrangia
poculata
.
We
compared
metrics
(melanin
synthesis,
antioxidant
production
and
antibacterial
activity)
between
colonies
varying
symbiont
density.
Symbiont
density
was
positively
correlated
both
melanin
concentration,
likely
as
a
result
dual
roles
pathways
symbiosis
regulation.
Our
results
confirm
complex
nature
relationships
highlight
need
for
nuanced
approaches
when
considering
relationships.
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 13, 2025
Abstract
Oxylipin
signaling
has
been
suggested
as
a
potential
mechanism
for
the
inter-partner
recognition
and
homeostasis
regulation
of
cnidarian-dinoflagellate
symbiosis,
which
maintains
ecological
viability
coral
reefs.
Here
we
assessed
effects
symbiosis
symbiont
identity
on
model
cnidarian,
sea
anemone
Exaiptasia
diaphana,
using
mass
spectrometry
to
quantify
octadecanoid
oxylipins
(i.e.,
18-carbon-derived
oxygenated
fatty
acids).
A
total
84
octadecanoids
were
reported,
distinct
stereospecificity
was
observed
synthesis
R-S-enantiomers
symbiont-free
anemones
free-living
cultured
dinoflagellate
symbionts,
respectively.
Symbiont-derived
13(S)-hydroxy-octadecatetraenoic
acid
(13(S)-HOTE)
linked
13(S)-lipoxygenase
translocated
host
with
32-fold
increase,
suggesting
it
biomarker
agonist
receptors
that
regulate
inflammatory
transcription.
Only
native
Breviolum
minutum
decreased
abundance
pro-inflammatory
9(R)-hydroxy-octadecadienoic
(9(R)-HODE)
in
host.
In
contrast,
non-native
Durusdinium
trenchii
marked
by
higher
autoxidation-derived
octadecanoids,
corroborating
previous
evidence
cellular
stress
this
association.
The
putative
pathways
reported
here
suggest
foundational
knowledge
gaps
can
support
bioengineering
selective
breeding
more
optimal
host-symbiont
pairings
enhance
resilience
survival
Development Growth & Differentiation,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 21, 2025
Abstract
In
previous
studies,
we
have
established
approximately
15
cultured
cell‐lines
derived
from
planula
larvae
of
Acropora
tenuis
.
Based
on
their
morphology
and
behavior,
these
cells
were
classified
into
three
types,
flattened
amorphous
(FAmCs),
vacuolated
adherent
(VAdCs),
small
smooth
(SSmCs).
FAmCs
include
fibroblast‐like
spherical,
brilliant
brown
(BBrCs),
which
are
transformable
to
each
other.
To
examine
the
larval
origin
cell
raised
antibodies:
anti‐AtMLRP2
that
appears
recognize
FAmC,
anti‐AtAHNAK
for
BBrC,
anti‐AtSOMP5
anti‐AtEndoG
SSmC,
anti‐AtGal
anti‐AtFat4
VAdC,
respectively.
Anti‐AtMLRP2
antibody
stained
in
vivo
stomodeum
neuroblast‐like
embedded
ectoderm
around
aboral
pole.
Anti‐AtAHNAK
neuron‐like
cells,
both
also
with
neuron‐specific
tubulin
β‐3
antibody.
These
results
suggest
vitro
BBrCs
share
neuronal
properties
common.
Two
antibodies
SSmCs,
anti‐AtEndoG,
suggesting
SSmCs
properties.
VAdCs,
anti‐AtFat4,
endoderm
VAdCs
Therefore,
lines
appear
retain
stomodeum,
neuroblast,
ectoderm,
or
endoderm.
Each
them
may
be
used
future
investigations
reveal
cellular
molecular
types
coral
larvae,
such
as
potential
symbiosis.
