Colocalization and potential interactions of Endozoicomonas and chlamydiae in microbial aggregates of the coral Pocillopora acuta

Science Advances, Journal Year: 2023, Volume and Issue: 9(20)

Published: May 17, 2023

Corals are associated with a variety of bacteria, which occur in the surface mucus layer, gastrovascular cavity, skeleton, and tissues. Some tissue-associated bacteria form clusters, termed cell-associated microbial aggregates (CAMAs), poorly studied. Here, we provide comprehensive characterization CAMAs coral Pocillopora acuta. Combining imaging techniques, laser capture microdissection, amplicon metagenome sequencing, show that (i) located tentacle tips may be intracellular; (ii) contain Endozoicomonas (Gammaproteobacteria) Simkania (Chlamydiota) bacteria; (iii) vitamins to its host use secretion systems and/or pili for colonization aggregation; (iv) distinct, but adjacent, CAMAs; (v) receive acetate heme from neighboring Endozoicomonas. Our study provides detailed insight into endosymbionts, thereby improving our understanding physiology health providing important knowledge reef conservation climate change era.

Language: Английский

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Probiotics reshape the coral microbiome in situ without detectable off-target effects in the surrounding environment

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: April 9, 2024

Beneficial microorganisms for corals (BMCs), or probiotics, can enhance coral resilience against stressors in laboratory trials. However, the ability of probiotics to restructure microbiome situ is yet be determined. As a first step elucidate this, we inoculated putative probiotic bacteria (pBMCs) on healthy colonies Pocillopora verrucosa Red Sea, three times per week, during 3 months. pBMCs significantly influenced microbiome, while surrounding seawater and sediment remained unchanged. The genera Halomonas, Pseudoalteromonas, Bacillus were enriched probiotic-treated corals. Furthermore, treatment also correlated with an increase other beneficial groups (e.g., Ruegeria Limosilactobacillus), decrease potential pathogens, such as Vibrio. all (treated non-treated) throughout experiment, could not track health improvements protection stress. Our data indicate that healthy, therefore stable, microbiomes restructured situ, although repeated continuous inoculations may required these cases. Further, our study provides supporting evidence that, at studied scale, have no detectable off-target effects near

Language: Английский

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Microbial-Based Therapies to Restore and Rehabilitate Disrupted Coral Health

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 181 - 195

Published: Jan. 1, 2025

Language: Английский

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Horizon scan of rapidly advancing coral restoration approaches for 21st century reef management

Emerging Topics in Life Sciences, Journal Year: 2022, Volume and Issue: 6(1), P. 125 - 136

Published: Feb. 4, 2022

Coral reef restoration activity is accelerating worldwide in efforts to offset the rate of health declines. Many advances have already been made practices centred on coral biology (coral restoration), and particularly those that look employ high adaptive state capacity corals order ensure rebuilding biomass also equip reefs with enhanced resilience future stress. We horizon scan state-of-play for many innovations underway across complex life cycle spans both asexual sexual reproduction — assisted evolution (manipulations targeted host host-associated microbes), biobanking, as well scalable propagation planting how these are different stages maturity support new 21st century management frameworks. Realising potential tools aids undoubtedly rests validating approaches their application continues scale. Whilst ecosystem service responses increased scaling still largely remain be seen, has delivered immense understanding coral-associated microbial long lagged behind other sciences.

Language: Английский

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Symbiosis and host responses to heating

Trends in Ecology & Evolution, Journal Year: 2022, Volume and Issue: 37(7), P. 611 - 624

Published: April 29, 2022

Language: Английский

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Advancing the protection of marine life through genomics

PLoS Biology, Journal Year: 2022, Volume and Issue: 20(10), P. e3001801 - e3001801

Published: Oct. 17, 2022

The rapid growth in genomic techniques provides the potential to transform how we protect, manage, and conserve marine life. Further, solutions boost resilience of species climate change other disturbances that characterize Anthropocene require transformative approaches, made more effective if guided by data. Although genetic have been employed conservation for decades availability data is rapidly expanding, widespread application still lags behind types. This Essay reviews genetics genomics utilized management initiatives ocean restoration, highlights success stories, presents a pathway forward enhance uptake protecting our oceans.

Language: Английский

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The Evolution of Coral Reef under Changing Climate: A Scientometric Review

Animals, Journal Year: 2023, Volume and Issue: 13(5), P. 949 - 949

Published: March 6, 2023

In this scientometric review, we employ the Web of Science Core Collection to assess current publications and research trends regarding coral reefs in relation climate change. Thirty-seven keywords for change seven were used analysis 7743 articles on The field entered an accelerated uptrend phase 2016, it is anticipated that will last next 5 10 years publication citation. United States Australia have produced greatest number field. A cluster (i.e., focused issue) showed bleaching dominated literature from 2000 2010, ocean acidification 2010 2020, sea-level rise, as well central Red Sea (Africa/Asia), 2021. Three different types appear based which are (i) most recent (2021), (ii) influential (highly cited), (iii) mostly (frequently article) Great Barrier Reef, found waters Australia, thought be subject reef research. Interestingly, climate-induced temperature changes “ocean warming” “sea surface temperature” significant dominant area.

Language: Английский

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Genomic exploration of coral-associated bacteria: identifying probiotic candidates to increase coral bleaching resilience in Galaxea fascicularis

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: Aug. 19, 2023

Reef-building corals are acutely threatened by ocean warming, calling for active interventions to reduce coral bleaching and mortality. Corals associate with a wide diversity of bacteria which can influence health, but knowledge specific functions that may be beneficial under thermal stress is scant. Under the oxidative theory bleaching, scavenge reactive oxygen (ROS) or nitrogen species (RNS) expected enhance resilience. Further, bacterial carbon export might substitute supply from algal photosymbionts, resilience facilitate recovery. To identify probiotic candidates, we sequenced genomes 82 pure-cultured were isolated emerging model Galaxea fascicularis.Genomic analyses showed isolates affiliated 37 genera. Isolates such as Ruegeria, Muricauda Roseovarius found encode genes synthesis antioxidants mannitol, glutathione, dimethylsulfide, dimethylsulfoniopropionate, zeaxanthin and/or β-carotene. Genes involved in RNS-scavenging many G. fascicularis-associated bacteria, represents novel finding several genera (including Pseudophaeobacter). Transporters suggested (semiSWEET) detected seven isolates, including Pseudovibrio Roseibium. range strains, strains Roseibium Roseovarius, revealed genomic features colonisation association host, secretion systems eukaryote-like repeat proteins.Our work provides an in-depth analysis functional potential identifies combinations traits coral's ability withstand bleaching. Identifying characterising critical development effective probiotics boost climate Video Abstract.

Language: Английский

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Lack of evidence for the oxidative stress theory of bleaching in the sea anemone, Exaiptasia diaphana, under elevated temperature

Coral Reefs, Journal Year: 2022, Volume and Issue: 41(4), P. 1161 - 1172

Published: April 6, 2022

Abstract To survive in nutrient-poor waters corals rely on a symbiotic association with intracellular microalgae. However, increased sea temperatures cause algal loss—known as coral bleaching—often followed by death. Some of the most compelling evidence support ‘oxidative stress theory bleaching’ comes from studies that exposed corals, cultures their endosymbionts, or model Exaiptasia diaphana to exogenous antioxidants during thermal stress. Here, we replicate these experiments using E. addition ascorbate + catalase, catechin, mannitol under ambient and elevated along an antioxidant-free control. In absence antioxidants, bleached no change reactive oxygen species (ROS) levels associated microalgal cells. Ascorbate catalase treatments rescued anemones bleaching, although ROS antioxidant temperature conditions. While bleaching was not changes net for symbionts, it is evident our findings excess connected phenotype were successful mitigating effects cnidarians. This understanding may assist applied research aims reduce impact climate reefs.

Language: Английский

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Advancing coral microbiome manipulation to build long-term climate resilience

Microbiology Australia, Journal Year: 2023, Volume and Issue: 44(1), P. 36 - 40

Published: March 9, 2023

Coral reefs house one-third of all marine species and are high cultural socioeconomic importance. However, coral under dire threat from climate change other anthropogenic stressors. Climate is causing bleaching, the breakdown symbiosis between host its algal symbionts, often resulting in mortality deterioration these valuable ecosystems. While it essential to counteract root causes change, remains urgent develop restoration conservation methods that will buy time for reefs. The manipulation bacterial microbiome associated with corals has been suggested as one intervention improve resilience. Early microbiome-manipulation studies, which aimed at enhancing bleaching tolerance, have shown promising results, but inoculated bacteria did generally not persist within microbiome. Here, we highlight importance long-term incorporation inocula into target corals, repeated inoculations be too costly feasible on large reef systems like Great Barrier Reef. Therefore, studies need prioritise approaches can provide sustained

Language: Английский

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