Microfluidic platform for microbial spore germination studies in multiple growth conditions DOI Creative Commons

Léa S. Bernier,

Aislinn Estoppey, Saskia Bindschedler

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1)

Published: Oct. 3, 2024

Language: Английский

Permafrost pore structure and its influence on microbial diversity: Insights from X-ray computed tomography DOI Creative Commons
Nathan David Blais, Joy M. O’Brien, Hannah Holland‐Moritz

et al.

Geoderma, Journal Year: 2025, Volume and Issue: 454, P. 117192 - 117192

Published: Feb. 1, 2025

Language: Английский

Citations

1
Salinity decreases the contribution of microbial necromass to soil organic carbon pool in arid regions DOI
Bin Jia,

Han Mao,

Yanmei Liang

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 930, P. 172786 - 172786

Published: April 25, 2024

Language: Английский

Citations

3
Millennial-Scale Depth-Resolved Ancient Microbial Diversity and Pathogenic Potential in Styx Glacier, Antarctica DOI
Minkyung Kim, Hanbyul Lee, S. Edward Lee

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Experimental Access to Cellulose Oxidation and the Dynamics of Microbial Carbon and Energy Use in Artificial Soil Under Varying Temperature, Water Content, and C/N ratio DOI Creative Commons
Shiyue Yang,

Alina Rupp,

Matthias Kästner

et al.

Soil Biology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109717 - 109717

Published: Jan. 1, 2025

Language: Английский

Citations

0
Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress DOI Creative Commons
Martina Lo Sterzo, Domenico Iuso, Luca Palazzese

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 27, 2025

Language: Английский

Citations

0
Isolation and characterization of Plasmodium falciparum blood-stage persisters by improved selection protocols using dihydroartemisinin alone DOI Creative Commons
Daniel Kiboi, Juliana M. Sá, Akshaykumar Nayak

et al.

Antimicrobial Agents and Chemotherapy, Journal Year: 2025, Volume and Issue: 69(3)

Published: Feb. 10, 2025

ABSTRACT Artemisinin-based combination therapies (ACTs) are vital for malaria treatment, but these threatened by blood-stage persisters—dormant forms of Plasmodium parasites that can survive drug exposure and cause recrudescent infections. Here, we present improved protocols efficient preparation pure falciparum persister populations without the need magnetically activated columns, sorbitol exposure, or prolonged manipulations. Our transformed actively replicating into exposing mixed to three four consecutive daily 6 h pulses 700 nM 200 dihydroartemisinin (DHA). In micrographs Giemsa-stained cells, observed different morphologies: Type I persisters containing a rounded magenta-stained nucleus accompanied local region blue-stained cytoplasm; more-prevalent II characterized dark round irregular-appearing faded no detectable cytoplasm. We also cells with disorganized nuclear cytoplasmic structure, suggesting possible autophagic processes destruction remodeling. Recrudescence starting parasitemia higher occurred around 17–22 days after initial DHA exposure. Differential expression patterns acetyl CoA carboxylase ( acc ) skeleton binding protein 1 sbp1 genes during dormancy, recrudescence highlighted evolution physiologic states metabolic changes underlying formation recovery. findings suggest hypotheses questions further research understand cellular pathways dormancy uncover strategies thwart parasite survival

Language: Английский

Citations

0
Characterization of RNA Helicase Genes in Ustilago maydis Reveals Links to Stress Response and Teliospore Dormancy DOI Open Access

Amanda M. Seto,

Barry Saville

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2432 - 2432

Published: March 8, 2025

Fungi produce dormant structures that are responsible for protection during adverse environmental conditions and dispersal (disease spread). Ustilago maydis, a basidiomycete plant pathogen, is model understanding the molecular mechanisms of teliospore dormancy germination. Dormant teliospores store components required germination including mRNAs which may be stored as dsRNAs. RNA helicases conserved enzymes function to modulate, bind, unwind duplexes, can displace other proteins. We hypothesize stabilize and/or modulate mRNAs. identified U. maydis udbp3 uded1 encoding interest they upregulated in decrease Experimental results suggest negative regulator osmotic stress-responsive genes modulates stress response by repressing translation. The altered expression also slow growth, polarized formation dsRNA. Together, data support role both modulating gene expression, stress, leading responses Increasing our these processes will aid developing novel strategies mitigate disease spread.

Language: Английский

Citations

0
Ecosystems have multiple interacting processes that buffer against co-occurring stressors DOI
Xiang Zheng Kong, Baile Xu, James Orr

et al.

Trends in Ecology & Evolution, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Atmospheric CO2 sink caused by enhanced chemical weathering in the Rongbuk glacier runoff at the initial ablation, Mt. Qomolangma (Everest) DOI
Haiying Qiu, Guangjian Wu, Zhengliang Yu

et al.

Journal of Hydrology, Journal Year: 2025, Volume and Issue: unknown, P. 133200 - 133200

Published: March 1, 2025

Language: Английский

Citations

0
The sleeping bacterium: shedding light on the resuscitation mechanism DOI Creative Commons
Eleonora Alfinito, Matteo Beccaria

European Biophysics Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Language: Английский

Citations

0