Deficiency in peptidoglycan recycling promotes β-lactam sensitivity in Caulobacter crescentus DOI Creative Commons

Malvika Modi,

Deepika Chauhan, Michael C. Gilmore

et al.

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

ABSTRACT Peptidoglycan (PG)-modifying enzymes play a crucial role in cell wall remodeling, essential for growth and division. Cell degradation products are transported to the cytoplasm recycled back most gram-negative bacteria, PG recycling is also linked β-lactam resistance many bacteria. Caulobacter crescentus intrinsically resistant β-lactams. Recently, it was shown that soluble lytic transglycosylase, SdpA, resistance. However, precise of SdpA unknown. This study investigated pathway its antibiotic C. . Anhydromuropeptides generated by action transglycosylases (LTs) permease AmpG. encodes an ampG homolog, deletion mutants sdpA sensitive The mutant displays significant accumulation anhydromuropeptides periplasm crescentus, demonstrating recycling. While single knockout exhibit no defects, double-deletion (∆ ∆ ) severe morphological defects. These double show enhanced sensitivity Analysis muropeptides wild-type (WT), , revealed reduced levels precursors (UDP-GlcNAc, UDP-MurNAc, UDP-MurNAc-P5), suggesting contribute toward de novo biosynthesis. Furthermore, supplementing media with GlcNAc sugar fitness under stress. In conclusion, our indicates defects compromise biogenesis, leading IMPORTANCE antibiotics target peptidoglycan biosynthetic response pressures, bacteria have developed various mechanisms. species, into induce expression β-lactamase enzymes. this study, we Based on data prior studies, propose utilized synthesis cytoplasm. A deficiency leads increased findings understanding mechanisms

Language: Английский

Deficiency in peptidoglycan recycling promotes β-lactam sensitivity in Caulobacter crescentus DOI Creative Commons

Malvika Modi,

Deepika Chauhan, Michael C. Gilmore

et al.

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

ABSTRACT Peptidoglycan (PG)-modifying enzymes play a crucial role in cell wall remodeling, essential for growth and division. Cell degradation products are transported to the cytoplasm recycled back most gram-negative bacteria, PG recycling is also linked β-lactam resistance many bacteria. Caulobacter crescentus intrinsically resistant β-lactams. Recently, it was shown that soluble lytic transglycosylase, SdpA, resistance. However, precise of SdpA unknown. This study investigated pathway its antibiotic C. . Anhydromuropeptides generated by action transglycosylases (LTs) permease AmpG. encodes an ampG homolog, deletion mutants sdpA sensitive The mutant displays significant accumulation anhydromuropeptides periplasm crescentus, demonstrating recycling. While single knockout exhibit no defects, double-deletion (∆ ∆ ) severe morphological defects. These double show enhanced sensitivity Analysis muropeptides wild-type (WT), , revealed reduced levels precursors (UDP-GlcNAc, UDP-MurNAc, UDP-MurNAc-P5), suggesting contribute toward de novo biosynthesis. Furthermore, supplementing media with GlcNAc sugar fitness under stress. In conclusion, our indicates defects compromise biogenesis, leading IMPORTANCE antibiotics target peptidoglycan biosynthetic response pressures, bacteria have developed various mechanisms. species, into induce expression β-lactamase enzymes. this study, we Based on data prior studies, propose utilized synthesis cytoplasm. A deficiency leads increased findings understanding mechanisms

Language: Английский

Citations

0