Microbiome responses to natural Fusarium infection in field-grown soybean plants DOI
Sietske van Bentum, Bridget S. O’Banion, Alexandra D. Gates

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

Abstract Aims The rhizosphere microbiome influences plant health, for example, by mediating plant-pathogen interactions. Plants can recruit protective microbes in response to disease, but the consistency of this process field conditions is unclear. We aimed identify candidate beneficial enriched during pathogen infection across multiple fields, offering potential support crop resilience against disease. Methods DNA amplicon sequencing was employed examine field-grown soybean (Glycine max L.) naturally infected with root pathogens three commercial fields Kentucky, USA. Symptomatic and asymptomatic plants were sampled assess disease-associated shifts bacterial fungal microbiome. Results identified a diverse Fusarium community, one Fusarium solani sequence variant (ASV) consistently diseased plants, identifying it as likely pathogen. While microbial communities differed between healthy these largely field-specific. Several ASVs known biocontrol (Clonostachys rosea, Penicillium, Trichoderma) implying role disease suppression. A Sphingomonas ASV, representing genus previously linked protection, more abundant rhizospheres two suggesting pathogen-triggered recruitment. Conversely, Macrophomina phaseolina, generalist pathogen, all indicating possible co-infection F. solani. Conclusions These findings reveal complex pathogen-associated patterns emphasize need field-specific research inform sustainable management strategies.

Language: Английский

Bring back the phenotype DOI
César Marín, Michael J. Wade

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Summary When thinking about evolutionary change, many practicing biologists will focus on changes in allele frequencies over time. This gene‐centric view of evolution has strongly impacted how (and biological science general) is thought, taught, and funded. In this viewpoint, we join recent criticisms the call for reinstalling a phenotypic evolution. The assumptions view—enormous/nonstructured populations totally random interactions between genes, individuals, environments—are hard to imagine real world. A gene's effects phenotype fitness depend its with other genes (epistasis), microbiome, environment, it generations, populations, environments. Incorrectly, have been given an agency role natural selection that they do not possess: replicate, but variation or differential proliferation through their traits (these are characteristics units deemed ‘interactors’). Here, show necessary capture several widespread phenomena: epistasis, nongenetic inheritance, multilevel selection, niche construction plant–soil feedbacks, all which vast empirical evidence. Life marvelous, complex, certainly more than machinery genetic information.

Language: Английский

Citations

0
Microbiome responses to natural Fusarium infection in field-grown soybean plants DOI
Sietske van Bentum, Bridget S. O’Banion, Alexandra D. Gates

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

Abstract Aims The rhizosphere microbiome influences plant health, for example, by mediating plant-pathogen interactions. Plants can recruit protective microbes in response to disease, but the consistency of this process field conditions is unclear. We aimed identify candidate beneficial enriched during pathogen infection across multiple fields, offering potential support crop resilience against disease. Methods DNA amplicon sequencing was employed examine field-grown soybean (Glycine max L.) naturally infected with root pathogens three commercial fields Kentucky, USA. Symptomatic and asymptomatic plants were sampled assess disease-associated shifts bacterial fungal microbiome. Results identified a diverse Fusarium community, one Fusarium solani sequence variant (ASV) consistently diseased plants, identifying it as likely pathogen. While microbial communities differed between healthy these largely field-specific. Several ASVs known biocontrol (Clonostachys rosea, Penicillium, Trichoderma) implying role disease suppression. A Sphingomonas ASV, representing genus previously linked protection, more abundant rhizospheres two suggesting pathogen-triggered recruitment. Conversely, Macrophomina phaseolina, generalist pathogen, all indicating possible co-infection F. solani. Conclusions These findings reveal complex pathogen-associated patterns emphasize need field-specific research inform sustainable management strategies.

Language: Английский

Citations

0