Replication Kinetics, Pathogenicity and Virus-induced Cellular Responses of Cattle-origin Influenza A(H5N1) isolates from Texas, United States DOI Creative Commons
Elsayed M. Abdelwhab, Ramya S. Barre, Anna Allué‐Guardia

et al.

Emerging Microbes & Infections, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

The host range of HPAIV H5N1 was recently expanded to include ruminants, particularly dairy cattle in the United States (US). Shortly after, human infection reported a worker Texas following exposure infected cattle. Herein, we rescued cattle-origin influenza A/bovine/Texas/24-029328-02/2024(H5N1, rHPbTX) and A/Texas/37/2024(H5N1, rHPhTX) viruses, identified human, respectively, their low pathogenic forms, rLPbTX rLPhTX, with monobasic HA cleavage sites. Intriguingly, rHPhTX replicated more efficiently than rHPbTX mammalian avian cells. Still, variations PA NA proteins didn't affect antiviral susceptibility inhibitors. Unlike rLPbTX, both rLPhTX exhibited higher pathogenicity efficient replication C57BL/6J mice. lungs rHPhTX-infected mice produced inflammatory cytokines/chemokines rHPbTX-infected Our results highlight potential risk virus adaptation and/or during current multistate/multispecies outbreak US.

Language: Английский

Emerging Threats of Highly Pathogenic Avian Influenza A (H5N1) in US Dairy Cattle: Understanding Cross-Species Transmission Dynamics in Mammalian Hosts DOI Creative Commons
Chithra Sreenivasan, Feng Li, Dan Wang

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(11), P. 1703 - 1703

Published: Oct. 30, 2024

The rapid geographic spread of the highly pathogenic avian influenza (HPAI) A(H5N1) virus in poultry, wild birds, and other mammalian hosts, including humans, raises significant health concerns globally. recent emergence HPAI agricultural animals such as cattle goats indicates ability to breach unconventional host interfaces, further expanding range. Among four types-A, B, C, D, are most susceptible D infection serve a reservoir for this seven-segmented virus. It is generally thought that bovines not hosts types viruses, type A. However, long-standing viewpoint has been challenged by outbreaks dairy cows United States. To date, into fourteen states, affecting 299 herds causing clinical symptoms reduced appetite, fever, sudden drop milk production. Infected can also transmit disease through raw milk. This review article describes current epidemiological landscape US its interspecies transmission events reported across globe. discusses viral determinants tropism, range, adaptative mutations various with natural experimental infections, vaccination strategies. Finally, it summarizes some immediate questions need be addressed better understanding biology, transmission, immune response bovines.

Language: Английский

Citations

2
Dissecting the role of the HA1-226 leucine residue in the fitness and airborne transmission of an A(H9N2) avian influenza virus DOI
Xiangjie Sun, Jessica A. Belser, Joanna A. Pulit-Penaloza

et al.

Journal of Virology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

ABSTRACT A better understanding of viral factors that contribute to influenza virus (IAV) airborne transmission is crucial for pandemic preparedness. limited capacity was recently observed in a human A(H9N2) isolate (A/Anhui-Lujiang/39/2018, AL/39) possesses leucine (L) residue at position HA1-226 (H3 numbering), indicative human-like receptor binding potential. To evaluate the roles this fitness and transmission, wild-type AL/39 (AL/39-wt) mutant (AL/39-HA1-L226Q) with single substitution from glutamine (Q), consensus avian viruses, were rescued assessed ferret model. The AL/39-HA1-L226Q lost ability transmit by air, although had comparable replication, induced similar levels host innate immune responses, detected air surrounding inoculated ferrets relative AL/39-wt virus. However, showed lower susceptibility infection compared Furthermore, viruses each gained dominance different anatomic sites respiratory tract co-infection competition model ferrets. Taken together, our findings demonstrate increasing HA1-L226 an plays multifaceted model, including improved infectivity. IMPORTANCE Although key prerequisite non-human origin become transmissible mammalian hosts, underlying molecular basis not well understood. In study, we investigated naturally occurring (leucine glutamine) 226 HA avian-origin impact on replication We enhanced associated mainly due increased infectivity Interestingly, found that, unlike most tract, ethmoid turbinate lined olfactory epithelium favors virus, suggesting site may serve as unique niche IAV avian-like specificity potentially allow spread extrapulmonary tissues facilitate adaptation hosts.

Language: Английский

Citations

2
Ecology and Evolution of Avian Influenza A Viruses in Wild Birds DOI
Michelle Wille

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 863 - 898

Published: Jan. 1, 2024

Language: Английский

Citations

1
Ptičja influenca u divljih kanida DOI Open Access
Ivana Lojkić, Andreja Jungić, Lorena Jemeršić

et al.

Veterinarska stanica, Journal Year: 2024, Volume and Issue: 55(6), P. 631 - 640

Published: April 29, 2024

The risk of cross-species transmission infectious diseases and zoonoses has increased due to the adaptive evolution pathogens anthropogenic landscape changes. A current example is avian influenza (AI) virus, which can infect not only species but also mammals. In these species, infections are often associated with severe neurological symptoms. AI viruses primarily well adapted mammalian hosts efficiently transmitted among them. To accomplish this, must acquire adaptations or mutations that contribute replication efficiency virulence in exact combination interaction result optimal adaptation mammals still unknown may vary between host virus subtypes. globally increasing number infected birds highly pathogenic (HPAI) increases likelihood contact mammals, especially wildlife. One reason for HPAI theexposure wild their carcasses. According data, canids, such as red foxes, most However, caused by neurotropic viruses, HPAI, have been scarcely studied at all jackals. Since jackals scavengers spread rapidly Europe Croatia recent years, surveillance infection canids utmost importance. Furthermore, interactions carnivores domestic animals concern, pose a public health threat adaptation.

Language: Английский

Citations

0
Replication Kinetics, Pathogenicity and Virus-induced Cellular Responses of Cattle-origin Influenza A(H5N1) Isolates from Texas, United States DOI Open Access

Ahmed Mostafa,

Ramya S. Barre, Anna Allué‐Guardia

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Abstract The host range of HPAIV H5N1 was recently expanded to include ruminants, particularly dairy cattle in the United States. Shortly after, human infection reported a worker Texas following exposure infected cattle. Herein, we rescued cattle-origin influenza A/bovine/Texas/24-029328-02/2024(H5N1, rHPbTX) and A/Texas/37/2024(H5N1, rHPhTX) viruses, identified human, respectively, their low pathogenic forms, rLPbTX rLPhTX, with monobasic HA cleavage sites. Intriguingly, rHPhTX replicated more efficiently than rHPbTX mammalian avian cells. Still, variations PA NA proteins didn’t affect antiviral susceptibility inhibitors. Compared rLPbTX, rLPhTX exhibited higher pathogenicity efficient replication C57BL/6J mice. lungs rHPhTX-infected mice produced inflammatory cytokines/chemokines rHPbTX-infected Our results highlight potential risk virus adaptation and/or during current multistate/multispecies outbreak.

Language: Английский

Citations

0
Global influenza threatens conservation DOI
Jonathan A. Runstadler

Science, Journal Year: 2024, Volume and Issue: 386(6722), P. 618 - 619

Published: Nov. 7, 2024

How avian influenza virus will continue to spread and circulate among wildlife is unclear.

Language: Английский

Citations

0
Replication Kinetics, Pathogenicity and Virus-induced Cellular Responses of Cattle-origin Influenza A(H5N1) isolates from Texas, United States DOI Creative Commons
Elsayed M. Abdelwhab, Ramya S. Barre, Anna Allué‐Guardia

et al.

Emerging Microbes & Infections, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

The host range of HPAIV H5N1 was recently expanded to include ruminants, particularly dairy cattle in the United States (US). Shortly after, human infection reported a worker Texas following exposure infected cattle. Herein, we rescued cattle-origin influenza A/bovine/Texas/24-029328-02/2024(H5N1, rHPbTX) and A/Texas/37/2024(H5N1, rHPhTX) viruses, identified human, respectively, their low pathogenic forms, rLPbTX rLPhTX, with monobasic HA cleavage sites. Intriguingly, rHPhTX replicated more efficiently than rHPbTX mammalian avian cells. Still, variations PA NA proteins didn't affect antiviral susceptibility inhibitors. Unlike rLPbTX, both rLPhTX exhibited higher pathogenicity efficient replication C57BL/6J mice. lungs rHPhTX-infected mice produced inflammatory cytokines/chemokines rHPbTX-infected Our results highlight potential risk virus adaptation and/or during current multistate/multispecies outbreak US.

Language: Английский

Citations

0