Differential Glial Response and Neurodegenerative Patterns in CA1, CA3, and DG Hippocampal Regions of 5XFAD Mice DOI Open Access
Tahsin Nairuz, Jin-Chul Heo, Jong‐Ha Lee

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12156 - 12156

Published: Nov. 12, 2024

In this study, the distinct patterns of glial response and neurodegeneration within CA1, CA3, dentate gyrus (DG) regions hippocampus were examined in 5XFAD mice at 6 12 months age. The primary feature transgenic mouse model is rapid onset amyloid pathology. We employed quantitative assessments via immunohistochemistry, incorporating double staining techniques, followed by observation with light microscopy subsequent digital analysis microscopic images. identified significantly increased Aβ deposition these three hippocampal mice. Moreover, CA1 CA3 showed higher vulnerability, signs reactive astrogliosis such as astrocyte density elevated GFAP expression. Additionally, we observed a significant rise microglia density, along inflammatory markers (TNFα) regions. These findings highlight non-uniform neuronal to plaque 5xFAD mice, potentially contributing neurodegenerative memory deficit characteristics Alzheimer's disease model.

Language: Английский

Astrocyte Regulation of Synaptic Plasticity Balances Robustness and Flexibility of Cell Assemblies DOI Creative Commons
Roman Koshkin, Tomoki Fukai

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 8, 2024

Abstract Cell assemblies are believed to represent the substrate of memory. Although long-term plasticity likely enables formation cell assemblies, how other factors, such as astrocytes and short-term (STP), affect their properties is poorly understood. To close this gap, we investigated assembly dynamics in a recurrent network model mimicking hippocampal area CA3. As shown experiment, connections our obey symmetric spike-timing-dependent (STDP), which weight change may or not depend on releasable amount neurotransmitter. The former case involves an interplay between STDP STP. In addition, implicitly modeled effect astrocyte NMDA receptors by manipulating breadth distribution neurotransmitter release probability Both STP-dependent STP-independent enabled spontaneous formation. Under former, however, tend be smaller more responsive external stimulation, improving network’s memory capacity enabling flexible restructuring. Furthermore, regulation facilitates stimulus-driven reorganization neural networks without destroying existing structure, thus balancing assemblies’ flexibility robustness. Our findings elucidate computational advantages interaction STP highlight astrocytes’ possible regulatory role

Language: Английский

Citations

0
Heterogeneous brain region-specific responses to astrocytic mitochondrial DNA damage in mice DOI Open Access

Daniela A. Ayala,

Anthony Matarazzo,

Bonnie L. Seaberg

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 31, 2024

Astrocytes use Ca

Language: Английский

Citations

0
Heterogeneous brain region-specific responses to astrocytic mitochondrial DNA damage in mice DOI Creative Commons

Daniela A. Ayala,

Anthony Matarazzo,

Bonnie L. Seaberg

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 10, 2024

Astrocytes display context-specific diversity in their functions and respond to noxious stimuli between brain regions. Astrocytic mitochondria have emerged as key players governing astrocytic functional heterogeneity, given ability dynamically adapt morphology regional demands on ATP generation Ca2+ buffering functions. Although there is reciprocal regulation mitochondrial dynamics signaling astrocytes, the extent of this astrocytes from different regions remains unexplored. Brain-wide, experimentally induced DNA (mtDNA) loss showed that mtDNA integrity critical for astrocyte function, however, possible diverse responses stimulus areas were not reported these experiments. To selectively damage a brain-region-specific manner, we developed novel adeno-associated virus (AAV)-based tool, Mito-PstI expressing restriction enzyme PstI, specifically mitochondria. Here, applied two regions, dorsolateral striatum dentate gyrus, show induces vivo, but with remarkable brain-region-dependent differences dynamics, fluxes, microglial reactivity. Thus, AAV-Mito-PstI tool explore relationship network brain-region-selective manner.

Language: Английский

Citations

0
Differential Glial Response and Neurodegenerative Patterns in CA1, CA3, and DG Hippocampal Regions of 5XFAD Mice DOI Open Access
Tahsin Nairuz, Jin-Chul Heo, Jong‐Ha Lee

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12156 - 12156

Published: Nov. 12, 2024

In this study, the distinct patterns of glial response and neurodegeneration within CA1, CA3, dentate gyrus (DG) regions hippocampus were examined in 5XFAD mice at 6 12 months age. The primary feature transgenic mouse model is rapid onset amyloid pathology. We employed quantitative assessments via immunohistochemistry, incorporating double staining techniques, followed by observation with light microscopy subsequent digital analysis microscopic images. identified significantly increased Aβ deposition these three hippocampal mice. Moreover, CA1 CA3 showed higher vulnerability, signs reactive astrogliosis such as astrocyte density elevated GFAP expression. Additionally, we observed a significant rise microglia density, along inflammatory markers (TNFα) regions. These findings highlight non-uniform neuronal to plaque 5xFAD mice, potentially contributing neurodegenerative memory deficit characteristics Alzheimer's disease model.

Language: Английский

Citations

0