The Long-Noncoding RNA TUG1 Regulates Oxygen-Induced Retinal Neovascularization in Mice via MiR-299 DOI Creative Commons
Yue Wang, Xue Wang, Yuexia Wang

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2022, Volume and Issue: 63(1), P. 37 - 37

Published: Jan. 27, 2022

Purpose: The oxygen-induced retinal neovascularization mouse model closely approximates pathological changes associated with human neovascularization-associated diseases, including retinopathies. We used this and endothelial cells (HRECs) under hypoxia to explore the relationship between taurine upregulated gene-1 (TUG1), vascular growth factor (VEGF), miR-299-3p on retinopathy of prematurity (ROP). Methods: An (OIR) was established; mice were divided into a normal control group, OIR TUG1 group (lentivirus control), TUG1-knockdown group. apoptosis evaluated using TUNEL assay. Angiogenic, apoptotic, inflammatory factors detected by Western blot, immunohistochemistry, immunofluorescence analyses. HRECs cultured assessed for VEGF expression, apoptosis, tubule formation, migration ability. TUG1, VEGF, via dual luciferase reporter gene Results: Intravitreal injection lentivirus reduced response in tissue markedly retina. Overexpression miR-299 rate, tube ability hypoxia-treated cells, thereby inhibiting formation new blood vessels. assay suggested that has binding sites VEGF. Conclusions: reduces expression VEGFA competitively adsorbing facilitates regulation neovascularization, suggesting it may serve as therapeutic target neovascular diseases.

Language: Английский

Inhaled non-viral delivery systems for RNA therapeutics DOI Creative Commons
Cheng Huang, Hongjian Li,

Xing Duan

et al.

Acta Pharmaceutica Sinica B, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0

Novel drug discovery strategies for chronic obstructive pulmonary disease: the latest developments DOI
Luigino Calzetta,

Elena Pistocchini,

Shima Gholamalishahi

et al.

Expert Opinion on Drug Discovery, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 10

Published: April 14, 2025

The journey from initial drug discovery to approval for respiratory diseases typically spans approximately 10.4 years and cost over $2.8 billion. This intricate process involves five stages: target identification, therapeutic molecule discovery, preclinical testing, clinical trials, regulatory approval. review examines novel strategies chronic obstructive pulmonary disease (COPD), focusing on advanced in vitro models that replicate human lung conditions accurate testing according the following search string: AND strategy COPD. It explores targeted molecular therapies, structure-based design, repurposing approaches facilitated by computational analysis. significance of personalized medicine tailoring treatments diverse COPDs is emphasized, highlighting complexity necessity these innovative methodologies improve outcomes. COPD remains a challenging area, with significant unmet medical need. Despite previous efforts, few effective therapies exist. Innovative models, are showing promise. Emphasizing existing drugs could transform treatment paradigms, promoting more complex like These innovations hold potential enhancing efficiency, leading precision approaches.

Language: Английский

Citations

0

Suppression of circXPO1 attenuates cigarette smoke-induced inflammation and cellular senescence of alveolar epithelial cells in chronic obstructive pulmonary disease DOI
Yong Du, Yi Ding,

Tianyun Shi

et al.

International Immunopharmacology, Journal Year: 2022, Volume and Issue: 111, P. 109086 - 109086

Published: July 27, 2022

Language: Английский

Citations

16

Identification and validation of aging-related genes in COPD based on bioinformatics analysis DOI Creative Commons
Shan Zhong, Yang Li, Naijia Liu

et al.

Aging, Journal Year: 2022, Volume and Issue: 14(10), P. 4336 - 4356

Published: May 24, 2022

Chronic obstructive pulmonary disease (COPD) is a serious chronic respiratory disorder. One of the major risk factors for COPD progression aging. Therefore, we investigated aging-related genes in using bioinformatic analyses. Firstly, Aging Atlas database containing 500 and Gene Expression Omnibus (GSE38974) were utilized to screen candidates. A total 24 candidate identified related both Using gene ontology Kyoto Encyclopedia Genes Genomes enrichment analyses, found that this list was enriched associated with cytokine activity, cell apoptosis, NF-κB IL-17 signaling. Four these (CDKN1A, HIF1A, MXD1 SOD2) determined be significantly upregulated clinical samples cigarette smoke extract-exposed Beas-2B cells vitro, their expression negatively correlated predicted forced expiratory volume vital capacity. In addition, combination levels four had good discriminative ability patients (AUC = 0.794, 95% CI 0.743–0.845). All as target hsa-miR-519d-3p, which down-regulated patients. The results from study proposed regulatory network hsa-miR-519d-3p/CDKN1A, MXD1, SOD2 closely COPD, provides theoretical basis link aging effectors progression, may suggest new diagnostic therapeutic targets disease.

Language: Английский

Citations

14

The Long-Noncoding RNA TUG1 Regulates Oxygen-Induced Retinal Neovascularization in Mice via MiR-299 DOI Creative Commons
Yue Wang, Xue Wang, Yuexia Wang

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2022, Volume and Issue: 63(1), P. 37 - 37

Published: Jan. 27, 2022

Purpose: The oxygen-induced retinal neovascularization mouse model closely approximates pathological changes associated with human neovascularization-associated diseases, including retinopathies. We used this and endothelial cells (HRECs) under hypoxia to explore the relationship between taurine upregulated gene-1 (TUG1), vascular growth factor (VEGF), miR-299-3p on retinopathy of prematurity (ROP). Methods: An (OIR) was established; mice were divided into a normal control group, OIR TUG1 group (lentivirus control), TUG1-knockdown group. apoptosis evaluated using TUNEL assay. Angiogenic, apoptotic, inflammatory factors detected by Western blot, immunohistochemistry, immunofluorescence analyses. HRECs cultured assessed for VEGF expression, apoptosis, tubule formation, migration ability. TUG1, VEGF, via dual luciferase reporter gene Results: Intravitreal injection lentivirus reduced response in tissue markedly retina. Overexpression miR-299 rate, tube ability hypoxia-treated cells, thereby inhibiting formation new blood vessels. assay suggested that has binding sites VEGF. Conclusions: reduces expression VEGFA competitively adsorbing facilitates regulation neovascularization, suggesting it may serve as therapeutic target neovascular diseases.

Language: Английский

Citations

13