Investigative Ophthalmology & Visual Science,
Journal Year:
2022,
Volume and Issue:
63(1), P. 37 - 37
Published: Jan. 27, 2022
Purpose:
The
oxygen-induced
retinal
neovascularization
mouse
model
closely
approximates
pathological
changes
associated
with
human
neovascularization-associated
diseases,
including
retinopathies.
We
used
this
and
endothelial
cells
(HRECs)
under
hypoxia
to
explore
the
relationship
between
taurine
upregulated
gene-1
(TUG1),
vascular
growth
factor
(VEGF),
miR-299-3p
on
retinopathy
of
prematurity
(ROP).
Methods:
An
(OIR)
was
established;
mice
were
divided
into
a
normal
control
group,
OIR
TUG1
group
(lentivirus
control),
TUG1-knockdown
group.
apoptosis
evaluated
using
TUNEL
assay.
Angiogenic,
apoptotic,
inflammatory
factors
detected
by
Western
blot,
immunohistochemistry,
immunofluorescence
analyses.
HRECs
cultured
assessed
for
VEGF
expression,
apoptosis,
tubule
formation,
migration
ability.
TUG1,
VEGF,
via
dual
luciferase
reporter
gene
Results:
Intravitreal
injection
lentivirus
reduced
response
in
tissue
markedly
retina.
Overexpression
miR-299
rate,
tube
ability
hypoxia-treated
cells,
thereby
inhibiting
formation
new
blood
vessels.
assay
suggested
that
has
binding
sites
VEGF.
Conclusions:
reduces
expression
VEGFA
competitively
adsorbing
facilitates
regulation
neovascularization,
suggesting
it
may
serve
as
therapeutic
target
neovascular
diseases.
Expert Opinion on Drug Discovery,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 10
Published: April 14, 2025
The
journey
from
initial
drug
discovery
to
approval
for
respiratory
diseases
typically
spans
approximately
10.4
years
and
cost
over
$2.8
billion.
This
intricate
process
involves
five
stages:
target
identification,
therapeutic
molecule
discovery,
preclinical
testing,
clinical
trials,
regulatory
approval.
review
examines
novel
strategies
chronic
obstructive
pulmonary
disease
(COPD),
focusing
on
advanced
in
vitro
models
that
replicate
human
lung
conditions
accurate
testing
according
the
following
search
string:
AND
strategy
COPD.
It
explores
targeted
molecular
therapies,
structure-based
design,
repurposing
approaches
facilitated
by
computational
analysis.
significance
of
personalized
medicine
tailoring
treatments
diverse
COPDs
is
emphasized,
highlighting
complexity
necessity
these
innovative
methodologies
improve
outcomes.
COPD
remains
a
challenging
area,
with
significant
unmet
medical
need.
Despite
previous
efforts,
few
effective
therapies
exist.
Innovative
models,
are
showing
promise.
Emphasizing
existing
drugs
could
transform
treatment
paradigms,
promoting
more
complex
like
These
innovations
hold
potential
enhancing
efficiency,
leading
precision
approaches.
Aging,
Journal Year:
2022,
Volume and Issue:
14(10), P. 4336 - 4356
Published: May 24, 2022
Chronic
obstructive
pulmonary
disease
(COPD)
is
a
serious
chronic
respiratory
disorder.
One
of
the
major
risk
factors
for
COPD
progression
aging.
Therefore,
we
investigated
aging-related
genes
in
using
bioinformatic
analyses.
Firstly,
Aging
Atlas
database
containing
500
and
Gene
Expression
Omnibus
(GSE38974)
were
utilized
to
screen
candidates.
A
total
24
candidate
identified
related
both
Using
gene
ontology
Kyoto
Encyclopedia
Genes
Genomes
enrichment
analyses,
found
that
this
list
was
enriched
associated
with
cytokine
activity,
cell
apoptosis,
NF-κB
IL-17
signaling.
Four
these
(CDKN1A,
HIF1A,
MXD1
SOD2)
determined
be
significantly
upregulated
clinical
samples
cigarette
smoke
extract-exposed
Beas-2B
cells
vitro,
their
expression
negatively
correlated
predicted
forced
expiratory
volume
vital
capacity.
In
addition,
combination
levels
four
had
good
discriminative
ability
patients
(AUC
=
0.794,
95%
CI
0.743â0.845).
All
as
target
hsa-miR-519d-3p,
which
down-regulated
patients.
The
results
from
study
proposed
regulatory
network
hsa-miR-519d-3p/CDKN1A,
MXD1,
SOD2
closely
COPD,
provides
theoretical
basis
link
aging
effectors
progression,
may
suggest
new
diagnostic
therapeutic
targets
disease.
Investigative Ophthalmology & Visual Science,
Journal Year:
2022,
Volume and Issue:
63(1), P. 37 - 37
Published: Jan. 27, 2022
Purpose:
The
oxygen-induced
retinal
neovascularization
mouse
model
closely
approximates
pathological
changes
associated
with
human
neovascularization-associated
diseases,
including
retinopathies.
We
used
this
and
endothelial
cells
(HRECs)
under
hypoxia
to
explore
the
relationship
between
taurine
upregulated
gene-1
(TUG1),
vascular
growth
factor
(VEGF),
miR-299-3p
on
retinopathy
of
prematurity
(ROP).
Methods:
An
(OIR)
was
established;
mice
were
divided
into
a
normal
control
group,
OIR
TUG1
group
(lentivirus
control),
TUG1-knockdown
group.
apoptosis
evaluated
using
TUNEL
assay.
Angiogenic,
apoptotic,
inflammatory
factors
detected
by
Western
blot,
immunohistochemistry,
immunofluorescence
analyses.
HRECs
cultured
assessed
for
VEGF
expression,
apoptosis,
tubule
formation,
migration
ability.
TUG1,
VEGF,
via
dual
luciferase
reporter
gene
Results:
Intravitreal
injection
lentivirus
reduced
response
in
tissue
markedly
retina.
Overexpression
miR-299
rate,
tube
ability
hypoxia-treated
cells,
thereby
inhibiting
formation
new
blood
vessels.
assay
suggested
that
has
binding
sites
VEGF.
Conclusions:
reduces
expression
VEGFA
competitively
adsorbing
facilitates
regulation
neovascularization,
suggesting
it
may
serve
as
therapeutic
target
neovascular
diseases.
