Potent phytoceuticals cocktail exhibits anti-inflammatory and antioxidant activity on LPS-triggered RAW264.7 macrophages in vitro DOI Creative Commons
Gabriele De Rubis, Keshav Raj Paudel,

Sofia Kokkinis

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 266, P. 155770 - 155770

Published: Dec. 13, 2024

Chronic inflammatory conditions, which include respiratory diseases and other ailments, are characterized by persistent inflammation oxidative stress, represent a significant health burden, often inadequately managed current therapies conventional inhaled bronchodilators oral or corticosteroids in the case of disorders. The present study explores potential Vedicinals®9 Advanced, polyherbal formulation, to mitigate LPS-induced stress RAW264.7 mouse macrophages. cells were pre-treated with followed exposure LPS induce an response. Key experimental outcomes assessed, including nitric oxide (NO) reactive oxygen species (ROS) production, as well expression stress-related genes proteins. Advanced significantly reduced NO ROS indicating strong anti-inflammatory antioxidant properties. Additionally, formulation downregulated LPS-upregulated mRNA pro-inflammatory cytokines, such TNF-α CXCL1, markers, GSTP1 NQO1. Furthermore, protein chemokines CCL2 CCL6, co-receptor, CD14, cytokines G-CSF IL-1β. These findings highlight therapeutic option for managing CRDs conditions. formulation's ability simultaneously target suggests it may offer advantages over existing treatments, broader application diseases.

Language: Английский

Applications and advancements of nanoparticle-based drug delivery in alleviating lung cancer and chronic obstructive pulmonary disease DOI
Gabriele De Rubis, Keshav Raj Paudel, Leander Corrie

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2023, Volume and Issue: 397(5), P. 2793 - 2833

Published: Nov. 22, 2023

Language: Английский

Citations

33
The Role of Transforming Growth Factor-β (TGF-β) in Asthma and Chronic Obstructive Pulmonary Disease (COPD) DOI Creative Commons
Krzysztof Kraik, Maciej Tota, Julia Laska

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(15), P. 1271 - 1271

Published: July 29, 2024

Asthma and chronic obstructive pulmonary disease (COPD) represent inflammatory respiratory disorders that, despite having distinct pathophysiological underpinnings, both feature airflow obstruction symptoms. A critical component in the pathogenesis of each condition is transforming growth factor-β (TGF-β), a multifunctional cytokine that exerts varying influences across these diseases. In asthma, TGF-β significantly involved airway remodeling, key aspect marked by subepithelial fibrosis, hypertrophy smooth muscle, enhanced mucus production, suppression emphysema development. The facilitates collagen deposition proliferation fibroblasts, which are crucial structural modifications within airways. contrast, role COPD more ambiguous. It initially acts as protective agent, fostering tissue repair curbing inflammation. However, prolonged exposure to environmental factors such cigarette smoke causes signaling malfunction. Such dysregulation leads abnormal excessive deposition, enlargement airspaces, and, thus, accelerated development emphysema. Additionally, epithelial-to-mesenchymal transition (EMT), process contributing phenotypic alterations observed COPD. thorough comprehension multifaceted asthma imperative for elaborating precise therapeutic interventions. We review several promising approaches alter signaling. Nevertheless, additional studies essential delineate further specific mechanisms its potential impacts

Language: Английский

Citations

9
A gelatin-chitosan-based film containing berberine hydrochloride/polypyrrole that promotes infectious wound healing through antibacterial and antioxidant properties, and electrical conductivity DOI
Yiqing Chen, Yingbin Lv, Wei Su

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141228 - 141228

Published: Feb. 1, 2025

Language: Английский

Citations

1
Plant-based therapeutics for chronic obstructive pulmonary diseases: Nanoformulation strategies to overcome delivery challenges DOI Creative Commons

Sofia Kokkinis,

Manisha Singh, Keshav Raj Paudel

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 58, P. 103761 - 103761

Published: Feb. 16, 2024

Chronic obstructive pulmonary disease (COPD) is a widespread global health problem marked by increasing airflow limitation and chronic airway inflammation. Conventional treatments for COPD offer limited efficacy have potentially undesirable effects, requiring the investigation of other therapeutic options. Because their anti-inflammatory, antioxidant, bronchodilatory plant-based chemicals emerged as viable candidates therapy. The successful delivery these compounds to respiratory system, on hand, remains an enormous challenge. This extensive review article explores promising potential therapies investigates leading-edge nanoformulation technologies targeted at addressing complex challenges that accompany natural compounds. We discuss multiple plant-derived (polyphenols, flavonoids, alkaloids) modes action in reducing COPD-related indications complications. Additionally, function nanotechnology improving bioavailability, stability, pharmaceuticals treatment explored. Nanoformulation techniques, such nanoparticles, liposomes, micelles, are described, with emphasis precisely encapsulate transport bioactive afflicted parts lung. Furthermore, constraints development nanoformulations also highlighted, including safety, scalability, regulatory issues. current aims provide thorough understanding future combining knowledge novel technologies. intersection nature-inspired medicines may hold key more effective, safer, patient-centred choices people suffering from this illness.

Language: Английский

Citations

4
Berberine Ameliorates Pulmonary Fibrosis by Inactivating the Thrombospondin 2/Smad Homolog 2/3 Pathway DOI
Liwen Zhang, Jie Chen, Xiang Huang

et al.

Chemical Biology & Drug Design, Journal Year: 2025, Volume and Issue: 105(4)

Published: April 1, 2025

ABSTRACT Pulmonary fibrosis (PF) is a progressive and irreversible lung disease. Previous studies have shown that berberine (BBR) ameliorates PF; however, the mechanism of BBR regulating PF remains unclear. mRNA expression thrombospondin 2 (THBS2) was analyzed by quantitative real‐time polymerase chain reaction. Protein level detected western blotting assay or immunohistochemistry assay. Cell function using cell counting kit‐8 assay, 5Ethynyl‐2'‐deoxyuridine transwell invasion wound‐healing enzyme‐linked immunosorbent assays, colorimetric mouse model established bleomycin (BLM) to analyze effect on PF. The results showed THBS2 upregulated in tissues BLM‐induced mice transforming growth factor‐β1 (TGF‐β1)‐induced HLF cells. ameliorated vivo. TGF‐β1 treatment induced proliferation, invasion, migration, inflammation response, oxidative stress, accompanied increases collagen I, fibronectin, α‐SMA protein expression; these effects were attenuated after silencing. In addition, TGF‐β1‐induced pro‐fibrotic phenotypes cells through inactivation THBS2/Smad2/3 pathway. Thus, inhibited inactivating pathway, providing theoretical basis for with BBR.

Language: Английский

Citations

0
Berberine suppressed the epithelial–mesenchymal transition (EMT) of colon epithelial cells through the TGF-β1/Smad and NF-κB pathways associated with miRNA-1269a DOI Creative Commons
Chao Huang,

H.-L. Liu,

Yidian Yang

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(16), P. e36059 - e36059

Published: Aug. 1, 2024

To explore the mechanisms of TGF-β1/Smad and NF-κB pathways in effect berberine (BBR) on colon cancer epithelial-mesenchymal transition (EMT) their regulatory relationships with microRNAs (miRNAs).

Language: Английский

Citations

2
Recent trends and therapeutic potential of phytoceutical‐based nanoparticle delivery systems in mitigating non‐small cell lung cancer DOI Creative Commons

Adam Haysom‐McDowell,

Keshav Raj Paudel, Stewart Yeung

et al.

Molecular Oncology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Lung cancer is the leading cause of death globally, with non‐small cell lung accounting for majority (85%) cases. Standard treatments including chemotherapy and radiotherapy present multiple adverse effects. Medicinal plants, used centuries, are traditionally processed by methods such as boiling oral ingestion, However, water solubility, absorption, hepatic metabolism reduce phytoceutical bioavailability. More recently, isolated molecular compounds from these plants can be extracted phytoceuticals administered either individually or an adjunct standard therapy. Phytoceuticals have been shown to alleviate symptoms, may dosage and, in some cases, enhance pharmaceutical mechanisms. Research has identified many phytoceuticals' actions on cancer‐associated pathways, oncogenesis, tumour microenvironment, proliferation, metastasis, apoptosis. The development novel nanoparticle delivery systems solid lipid nanoparticles, liquid crystalline liposomes enhanced bioavailability targeted pharmaceuticals phytoceuticals. This review explores biological pathways associated cancer, a diverse range phytoceuticals, they act upon, pros cons several systems.

Language: Английский

Citations

1
Fecal microbiome extract downregulates the expression of key proteins at the interface between airway remodelling and lung cancer pathogenesis in vitro DOI
Gabriele De Rubis, Keshav Raj Paudel, Sukriti Vishwas

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 260, P. 155387 - 155387

Published: June 1, 2024

Language: Английский

Citations

0
In silico Analysis of Berberine as a Potential Therapeutic Approach for Various Signalling Pathways Linked to Androgenetic Alopecia DOI
Sagarika Majhi,

Chaitanya Vinayak,

Iti Chauhan

et al.

Recent Advances in Inflammation & Allergy Drug Discovery, Journal Year: 2024, Volume and Issue: 19(1), P. 71 - 78

Published: July 10, 2024

Background: Alopecia is defined by a loss of hair density and often considered symptom multiple illnesses, such as infection inflammation. Objective: The molecular mechanisms underlying the hair-promoting effects include inhibition 5α-reductase activity, reducing binding affinity Dihydrotestosterone (DHT) to androgen receptors, decreasing/down-regulating TGF-β2 which have suggestive role in androgenetic alopecia. Finasteride minoxidil are approved non-surgical treatment alternatives for loss, but they cause side patients. Therefore, bioactive phytoconstituents with targets can be used find novel, secure, efficacious medicinal products. Methods: This study has carried out silico evaluation berberine using various software. To possible interactions between enzyme Transforming Growth Factor-- beta 2 (TGF-β2), critical protein involved human development cycle, computer-aided drug discovery was employed. Results: According studies, been found bind well 4K7a 6M2N sites. adhere Lipinski's rule five, its pharmacokinetic characteristics were noteworthy. Drug-likeness Absorption, Distribution, Metabolism, Excretion, Toxicity (ADMET) properties showed appreciable results. Furthermore, docking scores -8.4 (5α-reductase) -7.1 significantly better than (-4.8, -3.2). In general, exhibited improved interactions, toxicity investigations provided very little basis risk prediction. Conclusion: current protocol offered experimental support berberine's therapeutic use male pattern baldness. it target therapy alopecia through regulation activity.

Language: Английский

Citations

0
Cellular probing using phytoceuticals encapsulated advanced delivery systems in ameliorating lung diseases: Current trends and future prospects DOI Creative Commons

Jessica Katrine Datsyuk,

Gabriele De Rubis, Keshav Raj Paudel

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 141, P. 112913 - 112913

Published: Aug. 12, 2024

Chronic respiratory diseases such as Obstructive Pulmonary Disease (COPD) and asthma have posed a significant healthcare economic cost over prolonged duration worldwide. At present, available treatments are limited to range of preventive medicines, mono- or multiple-drug therapy, which necessitates daily use not considered viable reverse the inflammatory processes airway remodelling is inclusive alteration intra extracellular matrix tract, death epithelial cells, increase in smooth muscle cell activation fibroblasts. Hence, with problem mind considerable body study has been dedicated comprehending underlying factors that contribute inflammation within framework these disorders. adequate literature unveiled necessary cellular probing reduce tract by improving selectivity precision novel treatment. However, through mechanisms downregulation various markers, interleukin 8, (IL-8), Interleukin 6 (IL-6), 1β (IL-1β) tumor necrosis factor-α (TNF-α) uncovered. target probes implementation phytoceuticals encapsulated an advanced drug delivery system shown potential be solution vitro vivo studies highlighting their anti-inflammatory antioxidant effects. high costs associated systems focused exclusively on nanoparticles pose challenges. Additionally, biochemical characteristics due poor solubility, bioavailability, difficulties mass production makes it difficult implement this product treatment for COPD asthma. This aims examine integration many critical features context application chronic

Language: Английский

Citations

0