Woodsmoke and Diesel Exhaust: Distinct Transcriptomic Profiles in the Human Airway Epithelium
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 2, 2025
ABSTRACT
Climate
change
is
increasing
the
frequency
and
severity
of
wildfires
globally,
causing
significant
woodsmoke
(WS)
emissions.
Vehicles
emit
sizable
amounts
toxic
traffic-related
air
pollution
(TRAP),
for
which
diesel
exhaust
(DE)
a
model.
Both
WS
DE
contain
particulate
matter
<2.5
microns
(PM
2.5
)
deeply
penetrates
lungs,
respiratory
epithelial
inflammation
that
drives
health
effects.
Regulations
focus
on
PM
concentration,
despite
emerging
research
highlights
how
composition
mediates
As
are
compositionally
distinct,
we
conducted
first
head-to-head
comparison
effects
transcriptomes
air-liquid
interface
cultured
primary
human
bronchial
cells
(HBEC).
Differentiated
donor-matched
HBEC
transwells
were
exposed
2-hours
to
filtered
(FA;
control),
or
(furnace
tube
burning
pine)
(Hatz
1B30E
generator)
both
diluted
300
µg/m
3
.
had
higher
ultrafine
PM,
whereas
exposure
contained
significantly
NO
2
,
CO,
O
RNA
sequencing
showed
resulted
in
119
(↑41,
↓78)
differentially
expressed
genes,
while
modulated
399
(↑255,
↓144)
compared
FA
exposure.
was
associated
with
small
ribosomal
subunit
cytochrome
complex
related
HIF-1
signaling,
chain
interferon
alpha/beta
signaling/ISG15-protein
conjugation,
suggesting
TRAP
may
enhance
infection
risk.
We
also
analyzed
protein
immune-mediators.
demonstrate
two
major
sources
modulate
different
genes
pathways
HBECs,
minimal
overlap.
This
informs
debate
regarding
regulatory
concentration
assumptions
similar
concentrations
have
indistinct
Highlights
Lung
wood
smoke
underexplored
RNAseq
WS-exposed
lung
revealed
larger
greater
repression
host
antiviral
response-associated
than
A
miss
composition-specific
Language: Английский
Toxicological effects of long-term continuous exposure to ambient air on human bronchial epithelial Calu-3 cells exposed at the air-liquid interface
Elias J. Zimmermann,
No information about this author
Abhijit Das,
No information about this author
Alan H. Huber
No information about this author
et al.
Environmental Research,
Journal Year:
2025,
Volume and Issue:
unknown, P. 120759 - 120759
Published: Jan. 1, 2025
Language: Английский
On the dose-response association of fine and ultrafine particles in an urban atmosphere: toxicological outcomes on bronchial cells at realistic doses of exposure at the Air Liquid Interface
Chemosphere,
Journal Year:
2024,
Volume and Issue:
unknown, P. 143417 - 143417
Published: Sept. 1, 2024
Language: Английский
A mobile air-liquid interface exposure system optimized for outdoor toxicity testing of aerosols
Aerosol Science and Technology,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 13
Published: Dec. 2, 2024
Particulate
matter
(PM)
represents
a
growing
concern
in
public
health.
Air
Liquid
Interface
(ALI)
exposure
systems
are
used
to
assess
the
toxicity
of
airborne
pollutants
and
their
impact
on
human
However,
there
have
been
only
few
studies
using
ALI
outdoor
environments.
To
address
this
need,
we
developed
mobile
system
called
ELectrostatic
Air-Liquid
Exposure
(ELLIE)
system.
This
is
designed
deposit
particles,
especially
nanoparticles,
its
design
has
optimized
enable
field
studies.
Different
particle
size
fractions
can
be
targeted
by
different
selectors.
We
several
parameters,
such
as
aerosol
flow,
temperature,
humidity,
incubation
time,
ensure
best
cell
survival:
while
100
mL/min
doesn't
viability
no
relative
humidity
(within
tested
range),
even
maintained
at
flows
214
when
humidified
least
85%
humidity.
The
ELLIE
controls
temperature
standard
culture
conditions
(37
°C,
85–90%
RH).
Moreover,
it
enables
an
estimation
deposited
dose
layer
measuring
distribution
before
after
deposition
layer.
With
method,
copper
nanoparticles
was
estimated
1.5
µg/cm2
study.
Language: Английский
Portable emissions toxicity system: Evaluating the toxicity of emissions or polluted air by exposure of cell cultures at air-liquid interface in a compact field-deployable setup
The Science of The Total Environment,
Journal Year:
2024,
Volume and Issue:
959, P. 178010 - 178010
Published: Dec. 21, 2024
Language: Английский