Five glutathione S-transferase isozymes played crucial role in the detoxification of aflatoxin B1 in chicken liver DOI Creative Commons
Jiang Deng, Zhe Peng, Zhiyuan Xia

et al.

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 7, 2025

Abstract Background AFB 1 -8,9-exo-epoxide (AFBO) is the highly toxic product of Aflatoxin B (AFB ). Glutathione S -transferases (GSTs) play pivotal roles in detoxifying by catalyzing conjugation AFBO with glutathione (GSH). Although there are over 20 GST isozymes that have been identified chicken, involved detoxification process not yet. The objective this study was to determine which played key role . Results A total 17 pcDNA3.1(+)-GST isozyme plasmids were constructed and genes overexpressed 80–2,500,000 folds chicken Leghorn male hepatoma (LMH) cells. Compared treatment, overexpression GSTA2X, GSTA3, GSTT1L, GSTZ1-1, GSTZ1-2 increased cell viability 6.5%–17.0% LMH Moreover, five reduced release lactate dehydrogenase reactive oxygen species 8.8%–64.4%, 57.2%–77.6%, respectively, as well enhanced production AFBO-GSH 15.8%–19.6%, thus mitigating DNA damage induced After comprehensive evaluation various indicators, GSTA2X displayed best effects against expressed Pichia pastoris X-33 its enzymatic properties for GSH showed optimum temperature pH 20–25 °C 7.6–8.6 kinetic parameter V max 0.23 nmol/min/mg Michaelis constant 86.05 μmol/L substrate. Conclusions In conclusion, detoxification, will provide new remediation strategies prevent aflatoxicosis chickens.

Language: Английский

Poultry Nutrition: Achievement, Challenge and Strategy DOI
Kexin Cao,

Zhang‐Chao Deng,

Shijun Li

et al.

Journal of Nutrition, Journal Year: 2024, Volume and Issue: 154(12), P. 3554 - 3565

Published: Oct. 16, 2024

Language: Английский

Citations

4
Developmental changes of skin quality from breast, back, and thigh of Pekin ducks from 1 to 6 weeks of age DOI Creative Commons
Hao Li,

Wenxuan Zheng,

Shuo Xin

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: 104(2), P. 104759 - 104759

Published: Jan. 6, 2025

Good skin quality not only improved carcass but also increased consumer demand for fresh poultry meat. This study aimed to investigate the developmental changes in growth and of Pekin ducks during 1-6 weeks age. The samples were collected from breast, back, thigh tissues six male at end each week. strength, thickness, collagen content as well related gene expressions determined evaluation quality. results showed that body weight, absolute areas, density, epidermal dermal thickness (breast thigh), shearing force, piercing force (back linearly quadratically with age, reaching a plateau 5-6 age (P < 0.05). mRNA IGF-1 FGFR1 cell proliferation highest 3 while expression FGF14 EGF associated synthesis reached maximum values 5 Additionally, IGF1R FGFR2 upregulated breast skins 1 week old back compared birds other In conclusion, pattern structure linear manner was quadratic manner, which target genes synthesis.

Language: Английский

Citations

0
Five glutathione S-transferase isozymes played crucial role in the detoxification of aflatoxin B1 in chicken liver DOI Creative Commons
Jiang Deng, Zhe Peng, Zhiyuan Xia

et al.

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 7, 2025

Abstract Background AFB 1 -8,9-exo-epoxide (AFBO) is the highly toxic product of Aflatoxin B (AFB ). Glutathione S -transferases (GSTs) play pivotal roles in detoxifying by catalyzing conjugation AFBO with glutathione (GSH). Although there are over 20 GST isozymes that have been identified chicken, involved detoxification process not yet. The objective this study was to determine which played key role . Results A total 17 pcDNA3.1(+)-GST isozyme plasmids were constructed and genes overexpressed 80–2,500,000 folds chicken Leghorn male hepatoma (LMH) cells. Compared treatment, overexpression GSTA2X, GSTA3, GSTT1L, GSTZ1-1, GSTZ1-2 increased cell viability 6.5%–17.0% LMH Moreover, five reduced release lactate dehydrogenase reactive oxygen species 8.8%–64.4%, 57.2%–77.6%, respectively, as well enhanced production AFBO-GSH 15.8%–19.6%, thus mitigating DNA damage induced After comprehensive evaluation various indicators, GSTA2X displayed best effects against expressed Pichia pastoris X-33 its enzymatic properties for GSH showed optimum temperature pH 20–25 °C 7.6–8.6 kinetic parameter V max 0.23 nmol/min/mg Michaelis constant 86.05 μmol/L substrate. Conclusions In conclusion, detoxification, will provide new remediation strategies prevent aflatoxicosis chickens.

Language: Английский

Citations

0