Investigation of the Leaf and Pollen Micromorphology of Maples (Acer L.) of Iran DOI Creative Commons
Davoud Kartoolinejad,

F Shayanmehr,

Alireza Moshki

et al.

Risālaẗ al-Ḥusayn., Journal Year: 2024, Volume and Issue: 12(1), P. 124 - 137

Published: May 1, 2024

Background: The genus Acer L. (maples) from the Aceraceae are mostly deciduous trees, almost all of which distributed in temperate regions Northern Hemisphere.This contains 148-150 species around world, and presence 8-10 native this has been mentioned different floras Iran.Some maples Hyrcanian forests have great commercial value, height these trees reaches 40-45 m.Maples major vegetation elements most forest ecosystems Iran.Hyrcanian north Iran contain highest diversity genus.Despite molecular morphological studies on taxa no comprehensive conducted its micromorphological characteristics.Therefore, present research describes taxonomic value anatomical features leaf epidermis pollen morphology members Iran.Methods: Eleven maple available (nine two introduced taxa), mainly Zagros ecosystems, were collected analyzed current study.The investigated A. hyrcanum, velutinum, platanoides, mazandaranicum, campesstre, cappadocicum, negundo, palmatum, monspessulanum subsp.cinerascens, subsp.turcomanicum, subsp.ibericum.The samples each individual prepared branches grown sun dried with herbarium sample method to prevent mold.Dried placed boiling water for 3 min prepare dissections.Then, thin slices dorsal ventral middle vicinity midrib using a surgical blade.To remove color chlorophyll make transparent, they sodium hypochlorite solution 5 min.After washing distilled water, photographed studied under light microscope.For electron microscopy, epidermises stabled aluminum stubs.Then, very gold layer was sprinkled surface sputter coater then Philips Scanning Electron Microscope.The voltage intensity 20 kV, image magnification set 2500-5100 x.Epicuticular waxes, trichomes, epidermal cell wall shape, stomatal type, characteristics, density index classified microphotographs.Results: epicuticular wax absolutely unique such as velotinum, even some subspecies monspessulanum.The structured type waxes observed showing circular rosette-like structures formed stomata, could not be seen other taxa.The platanoides also uniquely covered completely smooth uniform wax.Certain surfaces subsp.cinerasens making cells appear prominent former indented latter number stomata per unit area belonged non-native species, namely palmatum parameters lowermost

Language: Английский

Atypical myopathy associated with box elder in Europe DOI Creative Commons
E. J. Knowles, Richard J. Piercy

Equine Veterinary Education, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Language: Английский

Citations

0
Release of Hypoglycin A from Hypoglycin B and Decrease of Hypoglycin A and Methylene Cyclopropyl Glycine Concentrations in Ruminal Fluid Batch Cultures DOI Creative Commons
Anna Maria Engel, Ahmed H. El‐Khatib, Martin Bachmann

et al.

Toxins, Journal Year: 2025, Volume and Issue: 17(2), P. 46 - 46

Published: Jan. 21, 2025

The transformation of hypoglycin A (HGA), B (HGB), and methylene cyclopropyl glycine (MCPrG) in ruminal fluid batch cultures was investigated, the effect these toxins on culture microorganisms using microbial metabolites measured. An experiment conducted ovine ANKOM RF Gas Production System over four runs, each with an incubation period 48 h. fermenters contained 200 mg (i) a substrate mixture (80% cellulose, 20% starch; CSM), (ii) CSM 1.5 mL solution pure (a 500 ng/mL HGA MCPrG each; PCM), or (iii) 100 sycamore maple seeds (SMS). Each fermenter 30 inoculum (ruminal buffer, 1:2 v/v). For control, autoclaved incubated CSM, PCM, SMS, respectively. Samples were taken from liquid phase analyzed chromatography–tandem mass spectrometry (LC/MS-MS) for metabolites. Microbial activity assessed gas production, short chain fatty acids, NH3 concentration. Additionally, pH redox potentials In concentrations rapidly decreased (p < 0.05), not measurable anymore after 24 h period. initial 4.7 ± 1.4 µg/mL HGA, 19.9 5.41 HGB, 1.2 0.33 MCPrG. increased h, coincidently to decrease HGB concentration 0.05). We modeled rapid conversion accompanied by progressive transformation. constant until 4 afterwards SMS incubations, 5.6 0.32 0.090 remained also occurred fluid. production metabolite higher compared PCM as used additional microorganisms.

Language: Английский

Citations

0
Horse with myopathy caused by consumption of box elder tree seedlings in the Czech Republic DOI Creative Commons
P. Jahn, Tereza Novotná, Radana Brumarová

et al.

Equine Veterinary Education, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 25, 2024

Summary An 18‐year‐old Saxon Warmblood gelding was referred to an equine clinic in the Czech Republic May 2019 for mild colic. The horse had 7 h of grazing and 30–45 min exercise every day. At clinic, physical examination ruled out gastrointestinal disease, while clinical pathology confirmed rhabdomyolysis (creatine kinase 29,088 IU/L; reference range 110–250 IU/L). Because suspicion atypical myopathy (AM), metabolomic analysis hypoglycin A (HGA), 31 acylcarnitines (ACs) free carnitine performed four dry serum one blood spot. After laboratory confirmation AM patient studied (HGA: 0.757–0.415 μmol/L, ACs elevation), incriminated pasture visited searched source toxin. No sycamore ( Acer pseudoplatanus ) found vicinity pasture. However, several box elder negundo trees were found, toxicological revealed HGA samaras (7.66–296.00 mg/kg), leaves (2.12–9.39 mg/kg) seedlings (339.00 mg/kg). Not only but also tree can be a potential poisoning horses Europe, particularly lowland areas with warm climate.

Language: Английский

Citations

1
Investigation of the Leaf and Pollen Micromorphology of Maples (Acer L.) of Iran DOI Creative Commons
Davoud Kartoolinejad,

F Shayanmehr,

Alireza Moshki

et al.

Risālaẗ al-Ḥusayn., Journal Year: 2024, Volume and Issue: 12(1), P. 124 - 137

Published: May 1, 2024

Background: The genus Acer L. (maples) from the Aceraceae are mostly deciduous trees, almost all of which distributed in temperate regions Northern Hemisphere.This contains 148-150 species around world, and presence 8-10 native this has been mentioned different floras Iran.Some maples Hyrcanian forests have great commercial value, height these trees reaches 40-45 m.Maples major vegetation elements most forest ecosystems Iran.Hyrcanian north Iran contain highest diversity genus.Despite molecular morphological studies on taxa no comprehensive conducted its micromorphological characteristics.Therefore, present research describes taxonomic value anatomical features leaf epidermis pollen morphology members Iran.Methods: Eleven maple available (nine two introduced taxa), mainly Zagros ecosystems, were collected analyzed current study.The investigated A. hyrcanum, velutinum, platanoides, mazandaranicum, campesstre, cappadocicum, negundo, palmatum, monspessulanum subsp.cinerascens, subsp.turcomanicum, subsp.ibericum.The samples each individual prepared branches grown sun dried with herbarium sample method to prevent mold.Dried placed boiling water for 3 min prepare dissections.Then, thin slices dorsal ventral middle vicinity midrib using a surgical blade.To remove color chlorophyll make transparent, they sodium hypochlorite solution 5 min.After washing distilled water, photographed studied under light microscope.For electron microscopy, epidermises stabled aluminum stubs.Then, very gold layer was sprinkled surface sputter coater then Philips Scanning Electron Microscope.The voltage intensity 20 kV, image magnification set 2500-5100 x.Epicuticular waxes, trichomes, epidermal cell wall shape, stomatal type, characteristics, density index classified microphotographs.Results: epicuticular wax absolutely unique such as velotinum, even some subspecies monspessulanum.The structured type waxes observed showing circular rosette-like structures formed stomata, could not be seen other taxa.The platanoides also uniquely covered completely smooth uniform wax.Certain surfaces subsp.cinerasens making cells appear prominent former indented latter number stomata per unit area belonged non-native species, namely palmatum parameters lowermost

Language: Английский

Citations

0