Development of an Easy-To-Use Microfluidic System to Assess Dynamic Exposure to Mycotoxins in 3D Culture Models: Evaluation of Ochratoxin A and Patulin Cytotoxicity DOI Creative Commons
Veronica Zingales, Caterina Piunti,

Sara Micheli

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(24), P. 4167 - 4167

Published: Dec. 23, 2024

Mycotoxins are among the most concerning natural toxic food contaminants. Over years, significant efforts have been made to characterize risk associated with their exposure. However, assessing toxicity has so far elusive due lack of adequate models that closely mimic physiological conditions human cells in vivo. Here, we present SpheroFlow Device (SFD), an efficient microfluidic platform designed, manufactured, and validated evaluate mycotoxin-induced cytotoxicity under dynamic continuous exposure 3D culture settings. In study, integrated neuroblastoma SH-SY5Y spheroids into SFD assess acute induced by mycotoxins ochratoxin A (OTA) patulin (PAT). The developed system enabled qualitative quantitative measurements concentration–response relationships provided accurate control over microenvironment. Our findings show enhancing model applying flow, exhibited different sensitivities OTA PAT compared conventional static spheroids, confirming critical role mycotoxin assessment. This is first study neurotoxicity on considering contribution fluid flow.

Language: Английский

Pyrroloquinoline Quinone Improved Boar Sperm Quality via Maintaining Mitochondrial Function During Cryopreservation DOI Creative Commons
Shanpeng Wang, Qi Wang, Lingjiang Min

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(1), P. 102 - 102

Published: Jan. 16, 2025

Due to oxidative damage and mitochondrial dysfunction, boar semen cryopreservation remains a significant challenge. This study investigates the effects of pyrroloquinoline quinone (PQQ), mitochondrial-targeted antioxidant, on post-thaw sperm quality during cryopreservation. Boar was diluted in freezing extender containing different concentrations PQQ (0, 10, 100, 1000, 10,000 nM). After freezing-thawing, motility, viability, acrosome integrity, activity, adenosine triphosphate (ATP) levels, DNA malondialdehyde (MDA) reactive oxygen species (ROS) superoxide dismutase (SOD) transcription proteins fertilization capacity were assessed. The results show that 1000 nM supplementation significantly enhanced integrity compared control (p < 0.05). Additionally, increased membrane potential (MMP) ATP while decreasing MDA ROS reducing Furthermore, levels mitochondrial-encoded elevated group Interestingly, showed higher binding rate oviductal epithelial cells zona pellucida (ZP), indicating potential. These findings suggest use mitochondria-target PQQ, can improve via its reduce stress protect function.

Language: Английский

Citations

2
Quercetin attenuates DEHP-induced pyroptosis and programmed necrosis in chicken duodenum through regulation of the TLR4/MyD88/NF-κB pathway DOI

Ruirui Xie,

Tong Xu, Yilin Yin

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: 372, P. 126016 - 126016

Published: March 6, 2025

Language: Английский

Citations

0
Development of an Easy-To-Use Microfluidic System to Assess Dynamic Exposure to Mycotoxins in 3D Culture Models: Evaluation of Ochratoxin A and Patulin Cytotoxicity DOI Creative Commons
Veronica Zingales, Caterina Piunti,

Sara Micheli

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(24), P. 4167 - 4167

Published: Dec. 23, 2024

Mycotoxins are among the most concerning natural toxic food contaminants. Over years, significant efforts have been made to characterize risk associated with their exposure. However, assessing toxicity has so far elusive due lack of adequate models that closely mimic physiological conditions human cells in vivo. Here, we present SpheroFlow Device (SFD), an efficient microfluidic platform designed, manufactured, and validated evaluate mycotoxin-induced cytotoxicity under dynamic continuous exposure 3D culture settings. In study, integrated neuroblastoma SH-SY5Y spheroids into SFD assess acute induced by mycotoxins ochratoxin A (OTA) patulin (PAT). The developed system enabled qualitative quantitative measurements concentration–response relationships provided accurate control over microenvironment. Our findings show enhancing model applying flow, exhibited different sensitivities OTA PAT compared conventional static spheroids, confirming critical role mycotoxin assessment. This is first study neurotoxicity on considering contribution fluid flow.

Language: Английский

Citations

0