A commentary on the inhibition of human TPC2 channel by the natural flavonoid naringenin: Methods, experiments, and ideas DOI Creative Commons
Velia Minicozzi, Tianwen Qi, Antonella Gradogna

et al.

BioMolecular Concepts, Journal Year: 2023, Volume and Issue: 14(1)

Published: Jan. 1, 2023

Abstract Human endo-lysosomes possess a class of proteins called TPC channels on their membrane, which are essential for proper cell functioning. This protein family can be functionally studied by expressing them in plant vacuoles. Inhibition hTPC activity naringenin, one the main flavonoids present human diet, has potential to beneficial severe diseases such as solid tumor development, melanoma, and viral infections. We attempted identify molecular basis interaction between hTPC2 using ensemble docking dynamics (MD) trajectories, but specific binding site remains elusive, posing challenge that could potentially addressed future increased computational power MD combined use microscopy techniques cryo-EM.

Language: Английский

Activating plant immunity: the hidden dance of intracellular Ca2+ stores DOI Open Access
Qi Wang, Xiao‐Yan Cang, Haiqiao Yan

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: 242(6), P. 2430 - 2439

Published: April 8, 2024

Calcium ion (Ca

Language: Английский

Citations

9
Function of Key Ion Channels in Abiotic Stresses and Stomatal Dynamics DOI Creative Commons

Yuanyuan Zuo,

Asad Abbas, Seidat Oluwadamilola Dauda

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109574 - 109574

Published: Jan. 1, 2025

Climate changes disrupt environmental and soil conditions that affect ionic balance in plants, presenting significant challenges to their survival productivity. Membrane transporters are crucial for maintaining homeostasis regulating the movement of substances across plasma organellar membranes, particularly under abiotic stresses. Among these stress-responsive mechanisms, stomata critical water loss carbon dioxide uptake, reflecting a plant's ability respond adapt stresses effectively. This review highlights role ion transporters, including both anion cation plant stress responses. It explores interplay between different channels regulatory components enable plants withstand key such as drought, salinity, heat. Moreover, we emphasized contributions three essential types - potassium, anion, calcium stress-related stomatal regulation. These orchestrate complex signaling networks allow modulate behavior maintain physiological adverse conditions. article provides valuable molecular insights into mechanisms transport regulation challenges. Thus, this offers useful foundation developing innovative strategies enhance crop resilience performance an era increasingly unpredictable harsh climates.

Language: Английский

Citations

0
In silico characterization of the gating and selectivity mechanism of the human TPC2 cation channel DOI Creative Commons
Alp Tegin Şahin, Ulrich Zachariae

The Journal of General Physiology, Journal Year: 2025, Volume and Issue: 157(3)

Published: Feb. 21, 2025

Two-pore channels (TPCs) are twofold symmetric endolysosomal cation forming important drug targets, especially for antiviral drugs. They activated by calcium, ligand binding, and membrane voltage, to date, they the only ion shown alter their selectivity depending on type of bound ligand. However, despite importance, activation TPCs molecular mechanisms underlying still poorly understood. Here, we set out elucidate mechanistic basis human TPC2 (hTPC2) mechanism ligand-induced channel lipid PI(3,5)P2. We performed all-atom in silico electrophysiology simulations study Na+ Ca2+ permeation across full-length hTPC2 timescale conduction investigated conformational changes induced presence or absence Our findings reveal that adopts distinct conformations PI(3,5)P2 allosteric transition pathways between these structures. Additionally, examined mechanism, solvation states, binding sites ions during through pore. The results our explain experimental observation is more selective over PI(3,5)P2via a multilayer mechanism. Importantly, mutations filter region maintain but change drastically.

Language: Английский

Citations

0
Wireless discharge of piezoelectric nanogenerator opens voltage-gated ion channels for calcium overload-mediated tumor treatment DOI
Yuchu He, Xiaoyu Yang, Meng Yuan

et al.

Biomaterials, Journal Year: 2025, Volume and Issue: unknown, P. 123311 - 123311

Published: April 1, 2025

Language: Английский

Citations

0
Calcium in plants: an important element of cell physiology and structure, signaling, and stress responses DOI Creative Commons
Agata Wdowiak, Anna Podgórska, Bożena Szal

et al.

Acta Physiologiae Plantarum, Journal Year: 2024, Volume and Issue: 46(12)

Published: Nov. 14, 2024

Abstract Calcium ions (Ca 2+ ) are absorbed from the soil by root cells before being distributed throughout plant. The transport of Ca to aboveground parts relies on movement xylem, which is influenced transpiration rate individual organs. final distribution in tissues and depends effective function channels transporters that facilitate through plasma specific intracellular membranes. Local fluctuations free concentrations serve as a mechanism elicit cellular responses, characterized distinct calcium signatures, or enable long-distance signaling cell-to-cell network. specificity proteins primarily determined studies plants with induced changes expression these proteins. Concurrently, it recognized plant contain significant reserves both labile permanently bound states, influences various aspects such biomechanical properties defensive capabilities cells. diverse roles highlight importance thorough understanding metabolism.

Language: Английский

Citations

3
Coordinating activation of endo‐lysosomal two‐pore channels and TRP mucolipins DOI Creative Commons
Yu Yuan, Dawid Jaślan, Taufiq Rahman

et al.

The Journal of Physiology, Journal Year: 2024, Volume and Issue: 602(8), P. 1623 - 1636

Published: April 1, 2024

Two-pore channels and TRP mucolipins are ubiquitous endo-lysosomal cation of pathophysiological relevance. Both Ca

Language: Английский

Citations

2
Shared signals, different fates: Calcium and ROS in plant PRR and NLR immunity DOI Creative Commons

Chanusha Weralupitiya,

Sophie Eccersall, Claudia‐Nicole Meisrimler

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(11), P. 114910 - 114910

Published: Oct. 30, 2024

Language: Английский

Citations

2
Conservation of Long-Range Signaling in Land Plants via Glutamate Receptor–Like Channels DOI Open Access
Masatsugu Toyota

Plant and Cell Physiology, Journal Year: 2024, Volume and Issue: 65(4), P. 657 - 659

Published: April 1, 2024

Language: Английский

Citations

1
Channels of Evolution: Unveiling Evolutionary Patterns in Diatom Ca2+ Signalling DOI Creative Commons
Eleanor A. Murphy, Friedrich H. Kleiner, Katherine E. Helliwell

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(9), P. 1207 - 1207

Published: April 26, 2024

Diatoms are important primary producers in marine and freshwater environments, but little is known about the signalling mechanisms they use to detect changes their environment. All eukaryotic organisms Ca2+ perceive respond environmental stimuli, employing a range of Ca2+-permeable ion channels facilitate movement across cellular membranes. We investigated distribution different families diatom genomes, with comparison other members stramenopile lineage. The four-domain voltage-gated (Cav) present some centric diatoms almost completely absent pennate diatoms, whereas single-domain EukCatA were found all diatoms. Glutamate receptors (GLRs) pentameric ligand-gated (pLGICs) also appear have been lost several species. Transient receptor potential (TRP) not undergone significant expansion seen brown algae. species analysed lacked mitochondrial uniporter (MCU), highly conserved channel type many eukaryotes, including lineages. These results highlight unique Ca2+-signalling toolkit indicate that evolutionary gains or losses may contribute differences cellular-signalling between

Language: Английский

Citations

0
A commentary on the inhibition of human TPC2 channel by the natural flavonoid naringenin: Methods, experiments, and ideas DOI Creative Commons
Velia Minicozzi, Tianwen Qi, Antonella Gradogna

et al.

BioMolecular Concepts, Journal Year: 2023, Volume and Issue: 14(1)

Published: Jan. 1, 2023

Abstract Human endo-lysosomes possess a class of proteins called TPC channels on their membrane, which are essential for proper cell functioning. This protein family can be functionally studied by expressing them in plant vacuoles. Inhibition hTPC activity naringenin, one the main flavonoids present human diet, has potential to beneficial severe diseases such as solid tumor development, melanoma, and viral infections. We attempted identify molecular basis interaction between hTPC2 using ensemble docking dynamics (MD) trajectories, but specific binding site remains elusive, posing challenge that could potentially addressed future increased computational power MD combined use microscopy techniques cryo-EM.

Language: Английский

Citations

1