Tandem mass tag (TMT)-based quantitative proteomics analysis reveals the different responses of contrasting alfalfa varieties to drought stress

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Aug. 27, 2024

Drought stress restricts the growth, distribution and productivity of alfalfa (Medicago sativa L.). In order to study response differences cultivars drought stress, we previously carried out physiological molecular comparative analysis on two varieties with contrasting resistance (relatively drought-tolerant Longdong drought-sensitive Algonquin). However, in proteomic factors still need be further studied. Therefore, TMT-based quantitative was performed using leaf tissues identify uncover differentially abundant proteins (DAPs). total, 677 DAPs were identified Algonquin 277 under stress. Subsequently, conducted various bioinformatics these DAPs, including subcellular location, functional classification biological pathway enrichment. The first main COG categories both after 'Translation, ribosomal structure biogenesis' 'Posttranslational modification, protein turnover, chaperones'. According KEGG database, treatment enriched different pathways. from 'photosynthesis' 'ribosome'. pathways 'linoleic acid metabolism', 'protein processing endoplasmic reticulum' 'RNA transport' significantly enriched. Finally, found significant DAP enrichment expression patterns between glycolysis/glycogenesis, TCA cycle, photosynthesis, biosynthesis, flavonoid isoflavonoid plant-pathogen interaction treatment. involved metabolic may explain These can used as candidate for breeding cultivate new germplasm more tolerance adapt unfavorable environments.

Language: Английский

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Comparative Evaluation of Paclobutrazol and Rhizobacterium Variovorax sp. YNA59 Effects on Mitigating Drought Stress in Chinese Cabbage (Brassica rapa ssp. Pekinensis)

Microbiology Research, Journal Year: 2023, Volume and Issue: 14(3), P. 1210 - 1224

Published: Aug. 24, 2023

Drought has severely impacted worldwide agricultural land, resulting in substantial yield loss and abiotic pressures crops such as Chinese cabbage. Paclobutrazol (PBZ) plant growth-stimulating bacteria have been extensively recommended to reduce a variety of stresses crops. Considering these facts, we used PBZ rhizobacterium Variovorax sp. YNA59, microbe with potential qualities, cabbage under drought stress non-stressed conditions, observed their impacts on morphological physiological attributes. Our findings revealed considerable improvement total biomass growth after treatment YNA59 (28%) (8%) conditions. In contrast, negative results were normal suppressing reducing biomass. The increase parameters, chlorophyll content, relative water photochemical efficiencies PS II, YNA59-treated plants, followed by PBZ, especially drought. Furthermore, significantly increased the endogenous phytohormone abscisic acid level, whereas inoculations decreased it. Antioxidant analysis that YNA glutathione catalase polyphenol oxidase peroxidase levels. These suggested could be more significant for conferring than PBZ.

Language: Английский

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Securing maize reproductive success under drought stress by harnessing CO2 fertilization for greater productivity

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 4, 2023

Securing maize grain yield is crucial to meet food and energy needs for the future growing population, especially under frequent drought events elevated CO 2 (eCO ) due climate change. To maximize kernel setting rate stress a key strategy in battling against negative impacts. Firstly, we summarize major limitations leaf source sink stress, identified that loss mainly attributed reduced set. Reproductive tolerance can be realized by collective contribution with greater assimilate import into ear, more available sugars ovary silk use, higher capacity remobilize reserve. As such, utilization of fertilization improved photosynthesis reserve remobilization coping change condition. We propose optimizing planting methods mining natural genetic variation still need done continuously, meanwhile, virtue advanced engineering plant phenomics tools, breeding program photosynthetic efficiency varieties adapted eCO accelerated. Consequently, stabilizing production achieved securing reproductive success harnessing fertilization.

Language: Английский

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Hormonal response to recurrent seasonal stress in coastal and mountain scabiouses growing in their natural habitat: linking ABA and jasmonates with photoprotection

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(2)

Published: March 1, 2024

Abstract Plant species distribution across ecosystems is influenced by multiple environmental factors, and recurrent seasonal stress events can act as natural selection agents for specific plant traits limit distribution. For that, studies aiming at understanding how constraints affect adaptive mechanisms of taxonomically closely related are great interest. We chose two Scabiosa inhabiting contrasting environments: the coastal scabious S. atropurpurea, typically coping with hot‐dry summers in a Mediterranean climate, mountain columbaria facing cold winters an oceanic climate. A set functional was examined to assess performance these congeneric from habitats. Both atropurpurea appeared be perfectly adapted their environment terms adjustments stomatal closure, CO 2 assimilation rate water use efficiency over seasons. However, unexpected dry period during winter followed typical summer forced plants deploy photoprotective responses summer. Aside reductions leaf content F v /F m , molecules (carotenoids, α‐tocopherol anthocyanins) per unit chlorophyll increased, mostly consequence severe loss. The profiling stress‐related hormones (ABA, salicylic acid jasmonates) revealed associations between ABA bioactive jasmonoyl‐isoleucine underlying response . conclude that jasmonates may used together trait may, least part, help understand current frame global climate change.

Language: Английский

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Moisture content and mycorrhizal fungi in maternal environment influence performance and composition of Lallemantia species offspring

Heliyon, Journal Year: 2024, Volume and Issue: 10(10), P. e31334 - e31334

Published: May 1, 2024

The availability of soil water content and nutrition in the maternal plant environment plays pivotal roles shaping performance, physio-biochemical properties, chemical composition produced seed. This study aimed to investigate effects arbuscular mycorrhizal fungi (AMF) on compositions Lallemantia species offspring. A split-factorial experiment was performed using a randomized complete block design (RCBD) with three replications. main plot consisted drought stress (30%, 60% 90% available depletion). subplots were factorial combination (AMF- AMF+) (L. iberica L. royleana). offspring both experienced decrease seed superoxide dismutase, catalase, ascorbate peroxidase enzyme activities, proline, as well rise hydrogen peroxide lipid peroxidation due limited environment. On other hand, providing adequate resulted improved germination index, increased starch, oil content, higher levels nitrogen phosphorus species. Compared royleana, had number achenes, seeds, weight, larger size, greater oil, nitrogen, phosphorus, potassium, calcium. In contrast, royleana exhibited longevity, enhanced under osmotic salinity stress, proline levels, activities antioxidant enzymes such ascorbic sucrose total soluble sugar. concludes that best carbohydrate observed depletion AMF inoculation These findings highlight significant impact offspring, valuable insights for optimizing production performance.

Language: Английский

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