Nitrogen fixation and transcriptome of a new diazotrophic Geomonas from paddy soils DOI Creative Commons
Guo‐Hong Liu, Shang Yang,

Shuang Han

et al.

mBio, Journal Year: 2023, Volume and Issue: 14(6)

Published: Oct. 19, 2023

ABSTRACT Nitrogen gas (N 2 ) fixation driven by diazotrophs is a crucial process for supplying nitrogen to paddy soil ecosystems. The genus Geomonas has been considered be an important potential diazotroph in soils, but direct experimental evidence of the nitrogen-fixing ability pure culture still lacking. Hence, we aimed demonstrate this capability and shed light on how was regulated response ammonium (NH 4 + . In study, determined that key nitrogenase gene ( nifH present 50 isolates from soils. Members contained minimum cluster nifBHDKEN based genomic analysis, implying species had fix nitrogen. Acetylene reduction assay (ARA), 15 N isotope labeling, total accumulation assays validated was, indeed, able culture. Under conditions, cell morphology changed short rod-shaped (with NH long flagella became longer thicker. expression genes correlated transcriptome quantified Expression associated with nitrogenase, flavin-based electron bifurcation complexes (such as FixAB system), uptake, transformation (e.g., glutamine glutamate synthetases) were significantly upregulated under suggesting these mechanisms might involved These results verified RT-qPCR. Taken together, our possess expand understanding ecological significance applications IMPORTANCE metabolic feature its application plant growth-promoting rhizobacterium. This research great importance it provides first comprehensive We isolated number strains soils strains. study demonstrated harbored encoding do Geobacter Anaeromyxobacter same class Deltaproteobacteria -dependent growth regulation fixation. establishes advances

Language: Английский

Assessment of Growth and Physiological Traits of Chenopodium quinoa Lines Under Different Salt Levels DOI Open Access

Mehwish Zafar,

Muhammad Shahzad, Muhammad Zubair Akram

et al.

Sarhad Journal of Agriculture, Journal Year: 2024, Volume and Issue: 40(2)

Published: Jan. 1, 2024

IntroductionC henopodium quinoa (Willd.) is cultivated mainly for its edible grains, considered as one of the crops that may be able to maintain food security in this century due remarkable nutritional qualities, such a high protein content, fiber, lipids, free gluten, having considerable amount fatty-acids, Abstract | Elevating salinity level has become worldwide threat agricultural lands.High concentration salts hampers growth plants and yield biomass by impacting major physiological mechanisms i.e., ionic, oxidative osmotic stress.One possible climate resilient strategy introduce new can bear allow irrigation with saline water.Quinoa great potential grow under conditions outstanding nutritious value.Pot based complete block design was conducted COMSATS University Abbottabad, Pakistan during winter season.Five lines (L30, L81, L11, L9, L24) were grown sandy loam soil containing 15 dS m -1 30dS NaCl salt treatments.The results revealed better noticed line L30 L24 30 dSm Salt treatments compared other three lines.Whereas, minimum plant genotype L9 L81 treatments.Highest leaf area, chlorophyll contents, contents noted all condition lines.High levels potassium, calcium magnesium lowest sodium aided resisting stress cause increased both non-saline soil.The present trial recognised maximum salt-tolerant severe stress, it might applied improve quinoa's tolerance later breeding phase.

Language: Английский

Citations

0

Localized nitrogen supply facilitates rice yield and nitrogen use efficiency by enabling root-zone nitrogen distribution and root growth DOI Creative Commons

Ren Hu,

Zijuan Ding,

Yingbing Tian

et al.

Frontiers in Sustainable Food Systems, Journal Year: 2024, Volume and Issue: 8

Published: April 10, 2024

Introduction Localized nitrogen (N) supply affects rice N uptake by influencing release, and few studies have examined the effects of root zone distribution growth on yield under localized (LNS). Methods A two-year field experiment was conducted with six treatments: no application, farmers’ fertilizer practice (FFP), four LNS treatments, including two types urea (U) controlled release (CRU) were mechanically side deep fertilized (SDF) or (RZF) at 10 cm soil depth (US, UR, CRUS CRUR respectively). Results Compared FFP, dry matter accumulation, uptake, increased 27%, 21%, 17%, respectively. For type, compared U, NH 4 + -N concentration, total surface area, volume, average diameter, biomass CRU significantly 50%, 43%, 53%, 23%, respectively, which resulted in a significant increase 12%. Regarding application methods, SDF 32%, 24%, 10%, 25% RZF, However, RZF more stable lasted longer, concentration 21% to SDF. Moreover, yield, agronomic use efficiency, gross return 3.15%, 5.62%, 2.81%, CRUS. Conclusion should be selected as recommended types, combination most effective choice for production.

Language: Английский

Citations

0

Seed Priming with Thiourea Enhances the Performance of Sesame (Sesamum indicum L.) Varieties Under Salinity Stress DOI Open Access

Bushra Irfan,

Muhammad Shahbaz,

Asif Mukhtiar

et al.

Sarhad Journal of Agriculture, Journal Year: 2024, Volume and Issue: 40(3)

Published: Jan. 1, 2024

IntroductionS esame (Sesamum indicum L.) belonging to the Pedaliaceae family possesses title queen of oilseeds owing its superior oil quality along with beneficial compounds like sterols, sesamin, sesamolin, and tocopherols, which serve as nutraceuticals contributing various physiological nutritional advantages (Langyan et al., 2022).The growth sesame plants is sustained by tropical, subtropical, southern temperate regions.Leading sesameexporting countries include China, Myanmar, India, Sudan (Chen 2020).Sesame can replace animal proteins fats used in human food Abstract | Salinity most significant abiotic factor limiting growth, physio-chemical mechanisms productivity.To address this issue a pot trial was conducted check effectiveness hydropriming (water; control), thiourea (150 mM) seed priming under normal (10.8 saline conditions (70 mM).The seeds two varieties (TS-05 TH-06) sown at Botany Garden, University Agriculture, Faisalabad.Results showed that salinity decreases (root shoot parameters) impaired balance between antioxidants (superoxide dismutase, catalase, peroxidase) oxidants (hydrogen peroxide malondialdehyde), lessened uptake essential minerals (potassium calcium) irrespective varietal differences.However, performance TH-6 better than TS-5.In addition, enhanced photosynthetic pigments efficiency, secondary metabolites production, antioxidant machinery, nutrient both increased development result.So, an effective strategy counteract negative effects salt stress improving tolerance mechanisms.

Language: Английский

Citations

0

QTL Mapping and Functional Identification of Candidate Genes Regulated by Sinorhizobium fredii HH103 and Associated with Nodulation Traits in Soybean DOI Creative Commons
Hejia Ni,

Siyi Tian,

Guoqing Zhang

et al.

Agronomy, Journal Year: 2023, Volume and Issue: 13(8), P. 2037 - 2037

Published: July 31, 2023

Large amounts of nitrogen fertilizer are annually applied to improve soybean yield. However, the overuse fertilizers has resulted in significant environmental pollution. Biological fixation is an ecological and environmentally friendly method increase signaling pathway function genes plant host regulated by rhizobia under symbiotic relationship remain unknown. In this study, chromosome region responsive Sinorhizobium fredii HH103 was identified using chromosome-segment-substituted lines produced from a cross between cultivated SN14 wild Zyd00006. addition, candidate associated with nodulation traits S. were identified. total, three quantitative trait loci (QTLs) containing 68 on chromosomes 02, 05, 20. The differentially expressed among QTL regions determined RNA-seq qRT-PCR. Glyma. 05G240500, potential gene responding positively regulating nodulation, To explore relationships haplotypes phenotypes, SNPs found regulatory areas Glyma.05G240500 haplotype investigated. Our study revealed role symbiosis provided reference for facilitating field marker-assisted selection.

Language: Английский

Citations

1

Nitrogen fixation and transcriptome of a new diazotrophic Geomonas from paddy soils DOI Creative Commons
Guo‐Hong Liu, Shang Yang,

Shuang Han

et al.

mBio, Journal Year: 2023, Volume and Issue: 14(6)

Published: Oct. 19, 2023

ABSTRACT Nitrogen gas (N 2 ) fixation driven by diazotrophs is a crucial process for supplying nitrogen to paddy soil ecosystems. The genus Geomonas has been considered be an important potential diazotroph in soils, but direct experimental evidence of the nitrogen-fixing ability pure culture still lacking. Hence, we aimed demonstrate this capability and shed light on how was regulated response ammonium (NH 4 + . In study, determined that key nitrogenase gene ( nifH present 50 isolates from soils. Members contained minimum cluster nifBHDKEN based genomic analysis, implying species had fix nitrogen. Acetylene reduction assay (ARA), 15 N isotope labeling, total accumulation assays validated was, indeed, able culture. Under conditions, cell morphology changed short rod-shaped (with NH long flagella became longer thicker. expression genes correlated transcriptome quantified Expression associated with nitrogenase, flavin-based electron bifurcation complexes (such as FixAB system), uptake, transformation (e.g., glutamine glutamate synthetases) were significantly upregulated under suggesting these mechanisms might involved These results verified RT-qPCR. Taken together, our possess expand understanding ecological significance applications IMPORTANCE metabolic feature its application plant growth-promoting rhizobacterium. This research great importance it provides first comprehensive We isolated number strains soils strains. study demonstrated harbored encoding do Geobacter Anaeromyxobacter same class Deltaproteobacteria -dependent growth regulation fixation. establishes advances

Language: Английский

Citations

1