ABSTRACT
Nitrogen
gas
(N
2
)
fixation
driven
by
diazotrophs
is
a
crucial
process
for
supplying
nitrogen
to
paddy
soil
ecosystems.
The
genus
Geomonas
has
been
considered
be
an
important
potential
diazotroph
in
soils,
but
direct
experimental
evidence
of
the
nitrogen-fixing
ability
pure
culture
still
lacking.
Hence,
we
aimed
demonstrate
this
capability
and
shed
light
on
how
was
regulated
response
ammonium
(NH
4
+
.
In
study,
determined
that
key
nitrogenase
gene
(
nifH
present
50
isolates
from
soils.
Members
contained
minimum
cluster
nifBHDKEN
based
genomic
analysis,
implying
species
had
fix
nitrogen.
Acetylene
reduction
assay
(ARA),
15
N
isotope
labeling,
total
accumulation
assays
validated
was,
indeed,
able
culture.
Under
conditions,
cell
morphology
changed
short
rod-shaped
(with
NH
long
flagella
became
longer
thicker.
expression
genes
correlated
transcriptome
quantified
Expression
associated
with
nitrogenase,
flavin-based
electron
bifurcation
complexes
(such
as
FixAB
system),
uptake,
transformation
(e.g.,
glutamine
glutamate
synthetases)
were
significantly
upregulated
under
suggesting
these
mechanisms
might
involved
These
results
verified
RT-qPCR.
Taken
together,
our
possess
expand
understanding
ecological
significance
applications
IMPORTANCE
metabolic
feature
its
application
plant
growth-promoting
rhizobacterium.
This
research
great
importance
it
provides
first
comprehensive
We
isolated
number
strains
soils
strains.
study
demonstrated
harbored
encoding
do
Geobacter
Anaeromyxobacter
same
class
Deltaproteobacteria
-dependent
growth
regulation
fixation.
establishes
advances
Sarhad Journal of Agriculture,
Journal Year:
2024,
Volume and Issue:
40(2)
Published: Jan. 1, 2024
IntroductionC
henopodium
quinoa
(Willd.)
is
cultivated
mainly
for
its
edible
grains,
considered
as
one
of
the
crops
that
may
be
able
to
maintain
food
security
in
this
century
due
remarkable
nutritional
qualities,
such
a
high
protein
content,
fiber,
lipids,
free
gluten,
having
considerable
amount
fatty-acids,
Abstract
|
Elevating
salinity
level
has
become
worldwide
threat
agricultural
lands.High
concentration
salts
hampers
growth
plants
and
yield
biomass
by
impacting
major
physiological
mechanisms
i.e.,
ionic,
oxidative
osmotic
stress.One
possible
climate
resilient
strategy
introduce
new
can
bear
allow
irrigation
with
saline
water.Quinoa
great
potential
grow
under
conditions
outstanding
nutritious
value.Pot
based
complete
block
design
was
conducted
COMSATS
University
Abbottabad,
Pakistan
during
winter
season.Five
lines
(L30,
L81,
L11,
L9,
L24)
were
grown
sandy
loam
soil
containing
15
dS
m
-1
30dS
NaCl
salt
treatments.The
results
revealed
better
noticed
line
L30
L24
30
dSm
Salt
treatments
compared
other
three
lines.Whereas,
minimum
plant
genotype
L9
L81
treatments.Highest
leaf
area,
chlorophyll
contents,
contents
noted
all
condition
lines.High
levels
potassium,
calcium
magnesium
lowest
sodium
aided
resisting
stress
cause
increased
both
non-saline
soil.The
present
trial
recognised
maximum
salt-tolerant
severe
stress,
it
might
applied
improve
quinoa's
tolerance
later
breeding
phase.
Frontiers in Sustainable Food Systems,
Journal Year:
2024,
Volume and Issue:
8
Published: April 10, 2024
Introduction
Localized
nitrogen
(N)
supply
affects
rice
N
uptake
by
influencing
release,
and
few
studies
have
examined
the
effects
of
root
zone
distribution
growth
on
yield
under
localized
(LNS).
Methods
A
two-year
field
experiment
was
conducted
with
six
treatments:
no
application,
farmers’
fertilizer
practice
(FFP),
four
LNS
treatments,
including
two
types
urea
(U)
controlled
release
(CRU)
were
mechanically
side
deep
fertilized
(SDF)
or
(RZF)
at
10
cm
soil
depth
(US,
UR,
CRUS
CRUR
respectively).
Results
Compared
FFP,
dry
matter
accumulation,
uptake,
increased
27%,
21%,
17%,
respectively.
For
type,
compared
U,
NH
4
+
-N
concentration,
total
surface
area,
volume,
average
diameter,
biomass
CRU
significantly
50%,
43%,
53%,
23%,
respectively,
which
resulted
in
a
significant
increase
12%.
Regarding
application
methods,
SDF
32%,
24%,
10%,
25%
RZF,
However,
RZF
more
stable
lasted
longer,
concentration
21%
to
SDF.
Moreover,
yield,
agronomic
use
efficiency,
gross
return
3.15%,
5.62%,
2.81%,
CRUS.
Conclusion
should
be
selected
as
recommended
types,
combination
most
effective
choice
for
production.
Sarhad Journal of Agriculture,
Journal Year:
2024,
Volume and Issue:
40(3)
Published: Jan. 1, 2024
IntroductionS
esame
(Sesamum
indicum
L.)
belonging
to
the
Pedaliaceae
family
possesses
title
queen
of
oilseeds
owing
its
superior
oil
quality
along
with
beneficial
compounds
like
sterols,
sesamin,
sesamolin,
and
tocopherols,
which
serve
as
nutraceuticals
contributing
various
physiological
nutritional
advantages
(Langyan
et
al.,
2022).The
growth
sesame
plants
is
sustained
by
tropical,
subtropical,
southern
temperate
regions.Leading
sesameexporting
countries
include
China,
Myanmar,
India,
Sudan
(Chen
2020).Sesame
can
replace
animal
proteins
fats
used
in
human
food
Abstract
|
Salinity
most
significant
abiotic
factor
limiting
growth,
physio-chemical
mechanisms
productivity.To
address
this
issue
a
pot
trial
was
conducted
check
effectiveness
hydropriming
(water;
control),
thiourea
(150
mM)
seed
priming
under
normal
(10.8
saline
conditions
(70
mM).The
seeds
two
varieties
(TS-05
TH-06)
sown
at
Botany
Garden,
University
Agriculture,
Faisalabad.Results
showed
that
salinity
decreases
(root
shoot
parameters)
impaired
balance
between
antioxidants
(superoxide
dismutase,
catalase,
peroxidase)
oxidants
(hydrogen
peroxide
malondialdehyde),
lessened
uptake
essential
minerals
(potassium
calcium)
irrespective
varietal
differences.However,
performance
TH-6
better
than
TS-5.In
addition,
enhanced
photosynthetic
pigments
efficiency,
secondary
metabolites
production,
antioxidant
machinery,
nutrient
both
increased
development
result.So,
an
effective
strategy
counteract
negative
effects
salt
stress
improving
tolerance
mechanisms.
Agronomy,
Journal Year:
2023,
Volume and Issue:
13(8), P. 2037 - 2037
Published: July 31, 2023
Large
amounts
of
nitrogen
fertilizer
are
annually
applied
to
improve
soybean
yield.
However,
the
overuse
fertilizers
has
resulted
in
significant
environmental
pollution.
Biological
fixation
is
an
ecological
and
environmentally
friendly
method
increase
signaling
pathway
function
genes
plant
host
regulated
by
rhizobia
under
symbiotic
relationship
remain
unknown.
In
this
study,
chromosome
region
responsive
Sinorhizobium
fredii
HH103
was
identified
using
chromosome-segment-substituted
lines
produced
from
a
cross
between
cultivated
SN14
wild
Zyd00006.
addition,
candidate
associated
with
nodulation
traits
S.
were
identified.
total,
three
quantitative
trait
loci
(QTLs)
containing
68
on
chromosomes
02,
05,
20.
The
differentially
expressed
among
QTL
regions
determined
RNA-seq
qRT-PCR.
Glyma.
05G240500,
potential
gene
responding
positively
regulating
nodulation,
To
explore
relationships
haplotypes
phenotypes,
SNPs
found
regulatory
areas
Glyma.05G240500
haplotype
investigated.
Our
study
revealed
role
symbiosis
provided
reference
for
facilitating
field
marker-assisted
selection.
ABSTRACT
Nitrogen
gas
(N
2
)
fixation
driven
by
diazotrophs
is
a
crucial
process
for
supplying
nitrogen
to
paddy
soil
ecosystems.
The
genus
Geomonas
has
been
considered
be
an
important
potential
diazotroph
in
soils,
but
direct
experimental
evidence
of
the
nitrogen-fixing
ability
pure
culture
still
lacking.
Hence,
we
aimed
demonstrate
this
capability
and
shed
light
on
how
was
regulated
response
ammonium
(NH
4
+
.
In
study,
determined
that
key
nitrogenase
gene
(
nifH
present
50
isolates
from
soils.
Members
contained
minimum
cluster
nifBHDKEN
based
genomic
analysis,
implying
species
had
fix
nitrogen.
Acetylene
reduction
assay
(ARA),
15
N
isotope
labeling,
total
accumulation
assays
validated
was,
indeed,
able
culture.
Under
conditions,
cell
morphology
changed
short
rod-shaped
(with
NH
long
flagella
became
longer
thicker.
expression
genes
correlated
transcriptome
quantified
Expression
associated
with
nitrogenase,
flavin-based
electron
bifurcation
complexes
(such
as
FixAB
system),
uptake,
transformation
(e.g.,
glutamine
glutamate
synthetases)
were
significantly
upregulated
under
suggesting
these
mechanisms
might
involved
These
results
verified
RT-qPCR.
Taken
together,
our
possess
expand
understanding
ecological
significance
applications
IMPORTANCE
metabolic
feature
its
application
plant
growth-promoting
rhizobacterium.
This
research
great
importance
it
provides
first
comprehensive
We
isolated
number
strains
soils
strains.
study
demonstrated
harbored
encoding
do
Geobacter
Anaeromyxobacter
same
class
Deltaproteobacteria
-dependent
growth
regulation
fixation.
establishes
advances