Micromachines,
Journal Year:
2022,
Volume and Issue:
13(2), P. 200 - 200
Published: Jan. 27, 2022
Microfluidic
technology
has
been
highly
useful
in
nanovolume
sample
preparation,
separation,
synthesis,
purification,
detection
and
assay,
which
are
advantageous
drug
development.
This
review
highlights
the
recent
developments
trends
microfluidic
applications
two
areas
of
First,
we
focus
on
how
microfluidics
developed
as
a
facile
tool
for
fabrication
carriers
including
microparticles
nanoparticles.
Second,
discuss
chips
could
be
used
an
independent
platform
or
integrated
with
other
technologies
toxicity
screening.
Challenges
future
perspectives
development
have
also
provided
considering
present
technological
limitations.
Biotechnology Journal,
Journal Year:
2020,
Volume and Issue:
15(5)
Published: Feb. 11, 2020
One
of
the
major
challenges
for
scientists
and
engineers
today
is
to
develop
technologies
improvement
human
health
in
both
developed
developing
countries.
However,
need
cost-effective,
high-performance
diagnostic
techniques
very
crucial
providing
accessible,
affordable,
high-quality
healthcare
devices.
In
this
context,
microfluidic-based
devices
(MFDs)
offer
powerful
platforms
automation
integration
complex
tasks
onto
a
single
chip.
The
distinct
advantage
MFDs
lies
precise
control
sample
quantities
flow
rate
samples
reagents
that
enable
quantification
detection
analytes
with
high
resolution
sensitivity.
With
these
excellent
properties,
microfluidics
(MFs)
have
been
used
various
applications
healthcare,
along
other
biological
medical
areas.
This
review
focuses
on
emerging
demands
MFs
different
fields
such
as
biomedical
diagnostics,
environmental
analysis,
food
agriculture
research,
etc.,
last
three
or
so
years.
It
also
aims
reveal
new
opportunities
areas
future
prospects
commercial
MFDs.
Lab on a Chip,
Journal Year:
2021,
Volume and Issue:
21(24), P. 4749 - 4759
Published: Jan. 1, 2021
Microfluidics
has
been
the
most
promising
platform
for
drug
screening
with
a
limited
number
of
cells.
However,
convenient
on-chip
preparation
wide
range
concentrations
remains
large
challenge
and
restricted
acceptance
microfluidics
in
precision
medicine.
In
this
paper,
we
report
digital
microfluidic
system
an
innovative
control
structure
chip
design
dispensing
to
generate
that
span
three
four
orders
magnitude,
enabling
single
or
combinatorial
multi-drug
simple
electronic
control.
Specifically,
utilize
droplet
ejection
from
drop
sitting
on
special
electrode,
named
dispenser,
under
high-voltage
pulse
actuation
deliver
desired
amount
drugs
be
picked
up
by
cell
suspension
driven
low-voltage
sine
wave
actuation.
Our
proof-of-principle
validation
technique
as
involved
testing
toxicity
two
chemotherapeutics,
cisplatin
(Cis)
epirubicin
(EP),
towards
MDA-MB-231
breast
cancer
cells
MCF-10A
normal
The
results
are
consistent
those
screened
based
traditional
96-well
plates.
These
findings
demonstrate
reliability
dispenser.
This
fewer
cells,
less
consumption,
small
footprint,
high
scalability
regard
concentration
could
pave
way
biopsied
primary
tumor
medicine
any
concentration-related
research.
Small,
Journal Year:
2021,
Volume and Issue:
17(46)
Published: Oct. 17, 2021
Abstract
Droplet
microfluidics
has
revolutionized
the
biomedical
and
drug
development
fields
by
allowing
for
independent
microenvironments
to
conduct
screening
at
single
cell
level.
However,
current
microfluidic
sorting
devices
suffer
from
drawbacks
such
as
high
voltage
requirements
(e.g.,
>200
Vpp),
low
biocompatibility,
and/or
throughput.
In
this
article,
a
single‐phase
focused
transducer
(SPFT)‐based
acoustofluidic
chip
is
introduced,
which
outperforms
many
droplet
through
energy
transmission
efficiency,
accuracy,
biocompatibility.
The
SPFT‐based
sorter
can
be
driven
with
an
input
power
lower
than
20
Vpp
maintain
postsorting
viability
of
93.5%.
SPFT
achieve
throughput
over
1000
events
per
second
purity
up
99.2%.
utilized
here
doxorubicin
cytotoxicity
on
cancer
noncancer
cells,
proving
its
capability.
Overall,
device
shows
great
potential
fast,
precise,
biocompatible
screening.
Scientific Reports,
Journal Year:
2020,
Volume and Issue:
10(1)
Published: June 23, 2020
Abstract
In
this
study,
we
present
a
straightforward
approach
for
functional
cell-based
screening
by
co-encapsulation
of
secretor
yeast
cells
and
reporter
mammalian
in
millions
individual
agarose-containing
microdroplets.
Our
system
is
compatible
with
ultra-high-throughput
selection
utilizing
standard
fluorescence-activated
cell
sorters
(FACS)
without
need
extensive
adaptation
optimization.
model
study
co-encapsulated
murine
interleukin
3
(mIL-3)-secreting
S.
cerevisiae
Ba/F3
cells,
which
express
green
fluorescent
protein
(GFP)
upon
stimulation
mIL-3,
could
observe
specific
robust
induction
fluorescence
signal
compared
to
control
secreting
non-functional
mIL-3
mutant.
We
demonstrate
the
successful
enrichment
activating
wt-secreting
from
1:10,000
dilution
expressing
inactive
cytokine
variant
two
consecutive
cycles
FACS.
This
indicates
suitability
presented
strategy
high-diversity
yeast-based
libraries
demonstrates
its
potential
efficient
isolation
clones
bioactive
recombinant
proteins.
Recent
years
have
seen
unparalleled
development
of
microfluidic
applications
for
antibody
discovery
in
both
academic
and
pharmaceutical
research.
Microfluidics
can
support
native
chain-paired
library
generation
as
well
direct
screening
secreting
cells
obtained
by
rodent
immunization
or
from
the
human
peripheral
blood.
While
broad
diversities
neutralizing
antibodies
against
infectious
diseases
such
HIV,
Ebola,
COVID-19
been
identified
convalescent
individuals,
microfluidics
expedite
therapeutic
cancer
immunological
disease
indications.
In
this
study,
a
commercially
available
device,
Cyto-Mine,
was
used
rapid
identification
natively
paired
rodents
donors
screened
specific
binding
to
recombinant
antigens,
with
expressing
target
interest,
and,
our
knowledge
first
time,
functional
IgG
droplets.
The
process
time
cell
preparation
confirmed
four
weeks.
Application
similar
devices
methodologies
accelerate
enhance
hit
discovery.
ACS Sustainable Chemistry & Engineering,
Journal Year:
2022,
Volume and Issue:
10(19), P. 6476 - 6482
Published: April 29, 2022
Metal–organic
frameworks
(MOFs)
have
recently
been
widely
used
in
antimicrobial
fields
due
to
their
adjustable
composition
and
morphology.
The
inherent
properties
of
MOFs
(e.g.,
shape,
size,
metal
element)
are
highly
related
antibacterial
performance.
traditional
beaker-based
synthesis
system
evaluate
often
leads
high
reagent
costs
environmental
pollution.
Here,
a
low-cost
droplet-based
microscale
platform
for
better
economical
screening
performance
is
demonstrated.
Multiple
(Zn-,
Co-,
Cu-MOFs)
with
controlled
morphologies
successfully
one-click
fabricated
through
such
platform.
Further
(Staphylococcus
aureus
Escherichia
coli)
the
above
indicates
that
Co-MOFs
show
effects
than
Zn-MOFs
Cu-MOFs.
Such
integration
droplet
array
can
greatly
reduce
cost
time,
especially
parallel
experiments,
provide
great
potential
guiding
large-scale
high-performance
materials.
