MrgX2-Targeting Ligand Screen for Antipseudoallergic Agents by Immobilized His-Tag-Fused Protein Technology

Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Mas-related G protein-coupled receptor X2 (MrgX2) plays a key role in pseudoallergy reactions; thus, it is of great significance to screen compounds with antipseudoallergy activity via MrgX2. Cell membrane chromatography (CMC) demonstrates potential drug screening, but requires further optimization improve its specificity and stability. In this study, new CMC system incorporating His-tag-oriented immobilized proteins was constructed MrgX2 antagonists. Single His-tag-fused extracted intactly covalently bond divinyl sulfone-modified amino silica gel obtain bioaffinity composites. The characterized composites were utilized establish MrgX2-His-tag@VS/CMC Compound Z-3578 screened from compound library 3010 revealed efficient vitro vivo conclusion, the oriented-immobilized will provide an analytical tool for screening active precursors.

Language: Английский

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Sustainable stable extraction of anti-osteoporotic compounds from Cornus officinalis by osteoblast membrane bionic multifunctional carbonaceous microspheres composite column

Journal of Food Composition and Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 107493 - 107493

Published: March 1, 2025

Language: Английский

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Mitochondrial membrane chromatography: Discovery of mitochondrial targeting modulators

Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101272 - 101272

Published: March 1, 2025

Language: Английский

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In silico and in vivo study of anti-inflammatory activity of Morinda longissima (Rubiaceae) extract and phytochemicals for treatment of inflammation-mediated diseases

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 328, P. 118051 - 118051

Published: March 16, 2024

Language: Английский

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Engineered Leukocyte Biomimetic Colorimetric Sensor Enables High-Efficient Detection of Tumor Cells Based on Bioorthogonal Chemistry

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(28), P. 36106 - 36116

Published: July 2, 2024

Accurate detection of heterogeneous circulating tumor cells (CTCs) is critical as they can make more aggressive, drug-resistant, and metastasizing. Although the leukocyte membrane coating strategy promising in meeting challenge detecting CTCs due to its inherent antiadhesive properties, it still limited by reduction or loss expression known markers. Bioorthogonal glycol-metabolic engineering expected break down this barrier feeding with sugar derivatives a unique functional group establish artificial targets on surface cells. Herein, an engineered biomimetic colorimetric sensor was accordingly fabricated for high-efficient CTCs. Compared conventional coating, could covalently bound models fed Ac4ManNAz vitro through synergy bioorthogonal chemistry metabolic glycoengineering, ignoring phenotypic changes Meanwhile, sandwich structure composed layer/CTCs/MoS2 nanosheet formed visual HeLa low 10 mL–1. Overall, approach overcome dependence cell technology specific phenotypes provide new viewpoint highly efficiently detect

Language: Английский

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Surface Membrane Coating as a Versatile Platform for Modifying Antitumor Nanoparticles

ACS Materials Letters, Journal Year: 2022, Volume and Issue: 4(10), P. 2019 - 2050

Published: Sept. 13, 2022

Antitumor nanoparticles now confront several obstacles, including poor biocompatibility and biodegradability, quick removal, a short cycle time, given the growing interest in surface membrane coating as versatile platform for altering overcoming limitations of current nanodrug delivery methods. This paper reviews recent progress cells, synthetic polymers, polyphenols, polysaccharides coatings with favorable biodegradability easy modification on antitumor nanoparticle platforms, which can provide promising strategy endowing specific expected performance to optimize efficiency antitumor. Furthermore, multiple combination strategies additional biorecognition ligands are supplied an attempt tackle challenges single variety tumor therapeutic applications ultimately facilitate effective clinical transformation.

Language: Английский

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Site-Specific Covalent Immobilization of SMA-Stabilized ACE2 for SARS-CoV-2 Recognition and Drug Screening

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(28), P. 33348 - 33361

Published: July 3, 2023

Membrane protein (MP)-based biomaterials have a wide range of applications in drug screening, antigen detection, and ligand–receptor interaction analysis. Traditional MP immobilization methods the disadvantage disordered orientation, leading to shielded binding domain unreliable pattern. Herein, we describe site-specific covalent MPs, which utilizes styrene maleic acid (SMA) detergent-free extraction method MPs as well reaction between His-tag divinyl sulfone (DVS). As an example, covalently immobilized angiotensin-converting enzyme 2 (ACE2) on cell membrane chromatography system (ACE2-His-SMALPs/CMC) manner verified specificity stability this system. This technique significantly improves service life compared physisorption CMC column. The improved strategies ACE2-His-SMALPs/CMC enable it effectively recognize SARS-CoV-2 pseudoviral particles detect viral ambient air once combined with aerosol collector; powerful ligand biosensor, was used screen for compounds anti-SARS-CoV-2 pseudovirus activity. In conclusion, optimized strategy has been successfully applied technology, showing enhanced sensitivity, can provide efficient convenient biomaterials.

Language: Английский

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Development of biomimetic phospholipid membrane chromatography for drug discovery: A comprehensive review

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 171, P. 117512 - 117512

Published: Dec. 28, 2023

Language: Английский

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Nondestructively Assemble Cell Membrane-Coated Nanoparticles by Host–Guest Interactions for Efficient Capture of Bioactive Compounds

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(43), P. 17353 - 17361

Published: Oct. 17, 2024

Cell membrane-coated nanoparticles (CNPs) have emerged as an attractive nanomedical tool. The basic premise is that the surface properties of natural cells can be integrated with physical and chemical by coating them cell membranes. However, degree preservation membrane proteins on nanoparticles, a key indicator related to biomedical function these biomimetic systems, largely affected process. Herein, we report supramolecular conjugation strategy mediated host-guest interactions assemble CNPs without compromising protein activities. β-cyclodextrin (β-CD) was rapidly stably inserted into lipid anchor affecting proteins, thus attaching sites surface. By harnessing excellent binding affinity between β-CD attached adamantane, membrane-magnetic nanoparticle conjugate (CDM@AMNPs) synthesized. Thanks nondestructive assembly this strategy, CDM@AMNPs were endowed greater number active sites, exhibiting efficient adsorption performance. This nonreceptor site-based proposes scalable cell-friendly for development highly CNPs.

Language: Английский

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Advancements in screening systems for active ingredients in traditional Chinese medicines using ligand fishing technique

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 117983 - 117983

Published: Sept. 1, 2024

Language: Английский

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