Plasmon‐Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High‐Throughput Discrimination of Pathogens

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Molecular diagnosis limitations, including complex treatment processes, low cost-effectiveness, and operator-dependent reproducibility, interrupt the timely prevention of disease spread development medical devices for home outdoor uses. A newly fabricated gold nanopillar array-based film is presented superior photothermal energy conversion. Magnifying metal surface-to-volume ratio increases conversion efficiency, resulting in a swift reduction gene amplification reaction time. Plasmonic energy-based ultrafast facile confirmation methodology offer rapid discrimination platform high-throughput multiplexed diagnosis. The performance arrayed demonstrated by measuring amount pathogen (Vibrio cholerae) with sensitivity 101 cfu mL-1 5.5 min. engineered can be utilized to diagnose universal pathogens achieve an increasingly successful complete cure.

Language: Английский

---

FEN1-Aided RPA (FARPA) Coupled with Autosampling Microfluidic Chip Enables Highly Multiplexed On-Site Pathogen Screening

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

A simple, rapid, low-cost, and multiplex detection platform is crucial for the diagnosis of infectious diseases, especially on-site pathogen screening. However, current methods are difficult to satisfy requirements minimal instrument multiplexed point-of-care testing (POCT). Herein, we propose a versatile easy-to-use (FARPA-chip) by combining FARPA with an autosampling microfluidic chip. pair universal recombinase polymerase amplification (RPA) primers introduced during double-stranded cDNA (ds-cDNA) preparation employed amplify multiple targets, followed amplicon-decoding chip, indicating no bias in amplifying different targets due RPA primers. FARPA-chip exhibits that as low 10 copies each target RNA starting sample can be sensitively detected 12-plex vector-borne viruses within 45 min cross-talk observed between targets. The feasibility this confirmed designing 9-plex detect 6 kinds clinically common respiratory from 16 clinical samples nasopharyngeal swabs, results completely consistent RT-qPCR. Furthermore, integrating quick extraction reagent, turnaround time significantly decreased <50 min, highlighting our enables cost-effective screening relatively high level multiplexing. Depending on number chambers design theoretically capable detecting up 24 pathogens, which should fulfill most purposes. We believe proposed could provide effective way series healthcare-related applications resource-limited settings.

Language: Английский

---

A new approach for the detection of genetic alterations utilizing modified loop-mediated isothermal amplification reaction (LAMP)

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 8, 2025

The increasing use of genetic testing for personalised therapy, highlights the need rapid, reliable diagnostics. Current methods are hindered by complex workflows, requiring advanced equipment, skilled personnel, and invasive tissue sampling. Loop-mediated isothermal amplification (LAMP) has emerged as a more efficient alternative to traditional PCR. LAMP eliminates thermal cycling, allowing faster, cost-effective tests, is less sensitive inhibitors, enabling from minimally processed samples. Although newer longer assay development time than PCR, its potential in oncology, particularly detecting changes, promising. We have developed LAMP-based method variations, optimized point-of-care testing. This technique uses modified primers with alterations at 3' end either F2 or B2 primers, ensuring specificity altered sequences. only produces positive signal when variant present, distinguishing it wild-type DNA. Our findings demonstrate that this high sensitivity, even samples both mutated material. Paired portable device, diagnostic could revolutionize alteration detection, offering quicker results improving treatment outcomes, targeted therapies.

Language: Английский

---

Large gold nanoparticle release assay for attomolar detection of miRNA related to myelodysplastic neoplasms

Talanta, Journal Year: 2025, Volume and Issue: unknown, P. 128037 - 128037

Published: March 1, 2025

Language: Английский

---

An Inkjet-Printed Nano Cellulose Electrochemical Biosensor

Springer Proceedings in Materials, Journal Year: 2025, Volume and Issue: unknown, P. 459 - 465

Published: Jan. 1, 2025

---