Journal of Physics D Applied Physics,
Journal Year:
2024,
Volume and Issue:
57(41), P. 415401 - 415401
Published: July 9, 2024
Abstract
The
immobilization
design
strategy
plays
an
important
role
in
biosensor
development
and
its
sensing
performance.
Both
adsorption
(physisorption)
cross-linker
functionalization
(chemisorption)
are
common
approaches
for
immobilizing
a
bioreceptor
layer.
In
this
work,
these
two
were
studied
compared,
envisaging
functional
strongly
attached
layer
onto
sputtered
Au–TiO
2
thin
films.
DSP
(Lomant’s
reagent)
was
used
the
film’s
functionalization,
effect
of
different
concentrations
on
adhesion
investigated.
Surface
morphology
analysis
functionalized
films
suggested
uniformly
coated
self-assembled
layers.
However,
islands
with
fractal
structure
found
concentration
4
mg
ml
−1
.
Infrared
spectroscopy
confirmed
at
surface.
Confocal
microscopy
immobilized
fluorescent
antibodies
revealed
that
improve
chemical
surface
area
available
immobilization.
Moreover,
immunoassay
using
mouse
IgG
interaction
anti-mouse
(Fab
specific),
working
as
capture
detection
antibody,
respectively,
showed
favors
orientation
strength
to
surface,
when
compared
physisorption.
Chemosensors,
Journal Year:
2023,
Volume and Issue:
11(7), P. 388 - 388
Published: July 12, 2023
Neurotransmitters
(NTs)
play
a
crucial
role
in
regulating
the
behavioral
and
physiological
functions
of
nervous
system.
Imbalances
concentrations
NT
have
been
directly
linked
to
various
neurological
diseases
(e.g.,
Parkinson’s,
Huntington’s,
Alzheimer’s
disease),
addition
multiple
psychotic
disorders
such
as
schizophrenia,
depression,
dementia,
other
neurodegenerative
disorders.
Hence,
rapid
real-time
monitoring
NTs
is
utmost
importance
comprehending
identifying
Among
different
sensing
techniques,
electrochemical
biosensors
garnered
significant
interest
due
their
ability
deliver
fast
results,
compatibility
for
miniaturization
portability,
high
sensitivity,
good
controllability.
Furthermore,
utilization
enzymes
recognition
elements
biosensing
design
has
renewed
attention
unique
advantages
catalytic
biorecognition
coupled
with
simultaneous
signal
amplification.
This
review
paper
primarily
focuses
on
covering
recent
advances
enzymatic
detection
NTs,
encompassing
sensors,
electrode
materials,
electroanalytical
techniques.
Moreover,
we
shed
light
applications
enzyme-based
complex
matrices
vivo
monitoring.
Despite
numerous
biosensors,
there
are
still
challenges
that
need
be
addressed,
which
thoroughly
discussed
this
paper.
Finally,
also
presents
an
outlook
future
perspectives
opportunities
development
detection.
