ChemistrySelect,
Journal Year:
2024,
Volume and Issue:
9(20)
Published: May 22, 2024
Abstract
Understanding
the
intricate
interplay
between
proteins
and
ligands
is
pivotal
for
rational
drug
development.
This
study
investigates
binding
of
Ofloxacin
to
Hb
using
diverse
biophysical
techniques:
fluorescence
spectroscopy,
UV‐visible
molecular
docking,
dynamics
simulations,
electrochemical
analysis.
Spectrophotometric
data
confirm
a
static
quenching
mechanism
in
ofloxacin‐Hb
binding.
Notably,
exhibits
significant
change
free
energy
(−17.134
kJ/mol)
strong
affinity
via
docking
was
also
observed
(−8.2
kcal/mol).
Molecular
simulations
validated
these
results,
showing
−91.093
kJ/mol
with
minimal
error
13.508
kJ/mol,
supporting
plausible
interaction.
Additionally,
cyclic
voltammetry
elucidated
dynamic
interactions
ofloxacin.
comprehensive
approach
provides
insights
into
drug‐protein
dynamics,
crucial
refining
development
strategies.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Feb. 21, 2024
Miniaturized
droplets,
characterized
by
well-controlled
microenvironments
and
capability
for
parallel
processing,
have
significantly
advanced
the
studies
on
enzymatic
evolution,
molecular
diagnostics,
single-cell
analysis.
However,
manipulation
of
small-sized
including
moving,
merging,
trapping
targeted
droplets
complex
biochemical
assays
subsequent
analysis,
is
not
trivial
remains
technically
demanding.
Among
various
techniques,
light-driven
methods
stand
out
as
a
promising
candidate
droplet
in
facile
flexible
manner,
given
features
contactless
interaction,
high
spatiotemporal
resolution,
biocompatibility.
This
review
therefore
compiles
an
in-depth
discussion
governing
mechanisms
underpinning
manipulation.
Besides,
light-responsive
materials,
representing
core
light-matter
interaction
key
character
converting
light
into
different
forms
energy,
are
particularly
assessed
this
review.
Recent
advancements
materials
most
notable
applications
comprehensively
archived
evaluated.
Continuous
innovations
rational
engineering
expected
to
propel
development
manipulation,
equip
with
enhanced
functionality,
broaden
routine
investigations.
Physics of Fluids,
Journal Year:
2025,
Volume and Issue:
37(2)
Published: Feb. 1, 2025
The
challenges
of
constraints
and
imprecision
in
chemical
analysis
medical
diagnosis
are
particularly
evident
the
separation
blood
cells.
Pinched
flow
fractionation,
a
passive
microfluidic
technique,
has
gained
attention
for
its
potential
size-based
cell
separation.
Enhancing
efficiency
is
crucial,
especially
through
integration
with
external
forces
or
active
methods.
This
study
explores
use
dielectrophoresis
(DEP)
magnetophoresis
(MP)
to
separate
platelet
cells,
red
white
circulating
tumor
These
were
analyzed
both
independently
simultaneously.
To
achieve
optimal
separation,
Navier–Stokes
equations,
Newton's
second
law,
system's
electric
magnetic
fields
modeled.
Experiments
conducted
branched
microchannel
assess
impact
various
parameters,
including
buffer
rate,
pinched
section
width,
applied
voltage,
field
frequency,
intensity.
revealed
approximately
99%
under
conditions
Wp=20
μm,
Q2=1250
μl/h,
f=100
kHz,
V=3
V,
M=1.5
T.
findings
demonstrate
that
while
DEP
MP
individually
enhance
their
simultaneous
application
significantly
improves
precision,
achieving
complete
optimized
system
holds
great
promise
applications
analytical
chemistry
diagnostics,
analysis.
LWT,
Journal Year:
2024,
Volume and Issue:
207, P. 116636 - 116636
Published: Aug. 15, 2024
In
this
research,
the
probiotic
potential,
anti-microbial,
anti-biofilm,
and
safety
properties
of
Levilactobacillus
brevis
Lb13H
were
investigated.
Also,
Gaussian
Process
Regression
(GPR)
model
was
applied
to
predict
some
experimental
parameters.
The
strain
demonstrated
resistance
acidic
pH,
gastric
intestinal
juices,
bile
salts.
Additionally,
exhibited
45.34%
surface
hydrophobicity,
36.55%
auto-aggregation
capacity,
26.30%
co-aggregation,
10.20%
adhesion
Caco-2
cells,
42.57%
cholesterol
removal,
48.63%
radical-scavenging
properties.
able
inhibit
Listeria
monocytogenes
cells
by
31.30%,
its
competitive
inhibition
percentage
37.5%.
Furthermore,
replacement
L.
with
measured
at
22.10%.
most
significant
antimicrobial
effect
observed
against
monocytogenes,
while
least
noted
Rhizopus
stolonifer.
Haemolytic
activity,
DNase
production,
biogenic
amine
production
not
in
strain,
which
also
found
be
sensitive
antibiotics
ciprofloxacin
chloramphenicol.
cell-free
supernatant
effectively
inhibited
degraded
biofilm
formation
achieving
effectiveness
rates
40.80%
36.60%
respectively.
results
modeling
indicated
that
there
is
any
difference
between
actual
predicted
data
GPR
can
variables
high
accuracy.
Pharmaceuticals,
Journal Year:
2024,
Volume and Issue:
17(6), P. 772 - 772
Published: June 13, 2024
The
emergence
of
antibiotic
resistance,
caused
by
the
improper
use
antibiotics,
is
a
significant
challenge
in
combating
infectious
diseases,
leading
to
millions
annual
fatalities.
occurrence
antimicrobial
side
effects
catalyzes
investigation
novel
compounds
and
sources
drugs.
Consequently,
research
on
biological
activity
that
conducted
plants,
plant
extracts,
are
produced
from
components
utmost
significance.
In
this
study,
CtAC/MNPs
were
obtained
reaction
activated
carbon
(AC)
fruits
Celtis
tournefortii
(Ct)
magnetic
nanoparticles
(MNPs),
CtAC/MNPs-Ag
nanocomposite
was
synthesized
reduction
silver
ions
added
reaction.
nanocomposites
analyzed
spectroscopically
(FTIR,
XRD),
microscopically
(SEM,
EDX),
optically
(DLS),
electrochemically
(zeta
potential)
magnetically
(VSM).
antibacterial
activities
against
S.
aureus
E.
coli
investigated
microdilution
method
using
minimal
inhibitory
concentration
(MIC)
disk
diffusion
methods.
Antioxidant
including
total
phenolic
content
DPPH
cuprac
assays,
revealed
remarkable
effect
nanocomposite.
This
study
has
advantages
obtaining
short
time
without
requiring
energy,
most
importantly,
takes
place
any
toxic
substances.
addition,
according
data
thought
shed
light
biomedical
research.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Feb. 27, 2024
Abstract
Nowadays,
droplet
microfluidics
has
become
widely
utilized
for
high-throughput
assays.
Efficient
mixing
is
crucial
initiating
biochemical
reactions
in
many
applications.
Rapid
during
formation
eliminates
the
need
incorporating
micromixers,
which
can
complicate
chip
design.
Furthermore,
immediate
of
substances
upon
contact
significantly
improve
consistency
chemical
and
resulting
products.
This
study
introduces
three
innovative
designs
generators
that
achieve
efficient
produce
small
droplets.
The
T-cross
cross-T
geometries
combine
cross
T
junction
mechanisms,
improved
efficiency.
Numerical
simulations
were
conducted
to
compare
these
novel
with
traditional
junctions
terms
index,
diameter,
eccentricity.
geometry
exhibited
highest
index
produced
smallest
For
flow
rate
ratio
0.5,
this
offered
a
10%
increase
decrease
diameter
by
compared
junction.
While
best
efficiency
among
generators,
it
produces
larger
droplets,
risk
contamination
due
microchannel
walls.
Therefore,
highly
desirable
most
applications
its
production
considerably
smaller
asymmetric
8%
around
2%
conventional
0.5.
All
demonstrated
comparable
lowest
droplets
(around
1%).
Thus,
geometries,
particularly
geometry,
are
favorable
choice
where
both
high
sizes
important.
