High-throughput analysis of hazards in novel food based on the density functional theory and multimodal deep learning

Food Chemistry, Journal Year: 2024, Volume and Issue: 442, P. 138468 - 138468

Published: Jan. 19, 2024

Language: Английский

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A review of microbial responses to biochar addition in anaerobic digestion system: Community, cellular and genetic level findings

Bioresource Technology, Journal Year: 2023, Volume and Issue: 391, P. 129929 - 129929

Published: Nov. 2, 2023

Language: Английский

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Development of a method for the quantification of six mycotoxins in cereal samples using isotope-coded derivatization combined with ultra-high-performance liquid chromatography–tandem mass spectrometry

Journal of Food Composition and Analysis, Journal Year: 2023, Volume and Issue: 120, P. 105347 - 105347

Published: April 14, 2023

Language: Английский

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Isotopic Labeling Analysis using Single Cell Mass Spectrometry

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract Here we show that a recently developed single cell mass spectrometry method can be used to monitor the incorporation of an isotopically labeled precursor into plant protoplasts. Specifically, deuterium-labeled tryptamine alkaloid pathway over course 24 hours. The resulting data provides glimpse rate synthesis and transport chemically complex monoterpene indole alkaloids in cells across small population. By measuring concentration alkaloids, gain insight flux important biosynthetic pathway. Stable-isotope labeling is widely approach study metabolic networks by tracking isotopologues time. This manuscript proof principle isotopic performed pathways.

Language: Английский

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Dynamic single-cell metabolomics reveals cell-cell interaction between tumor cells and macrophages

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 16, 2025

Language: Английский

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Analysis of the response mechanisms of Pinellia ternata to terahertz wave stresses using transcriptome and metabolic data

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 12, 2023

Pinellia ternata (Thunb.) Breit. (Araceae), a significant medicinal plant, has been used to treat various diseases for centuries. Terahertz radiation (THZ) is located between microwaves and infrared rays on the electromagnetic spectrum. THZ possesses low single-photon energy spectral fingerprint, but its effects plant growth have not yet investigated. The study's primary objective was examine transcriptome metabolome databases of SY line provide new perspective identifying genes associated with resistance promotion comprehending underlying molecular mechanism. Variations in biological characteristics P. grown under control experimental conditions were analyzed determine effect THZ. Compared group, phenotypic variables such as leaf length, petiole number leaves, diameter, proliferation coefficient exhibited differences. response regarding coercions root exudation. group contained considerably more sugar alcohol than group. analysis revealed 1,695 differentially expressed (DEGs), including 509 upregulated 1,186 downregulated genes. In KEGG-enriched hormone signaling pathway, there 19 genes, 13 which six upregulated. metabolomic analysis, approximately 416 metabolites uncovered. There 112 DEMs that downregulated, whereas 148 leaves displayed differences phytohormone metabolites, specifically brassinolide (BR) abscisic acid (ABA). rise BR triggers alterations internal hormones, resulting faster development ternata. Our findings demonstrated link several metabolic pathway processes, will enhance our understanding mechanisms.

Language: Английский

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Identification of Predictive Biomarkers of Lameness in Transition Dairy Cows

Animals, Journal Year: 2024, Volume and Issue: 14(14), P. 2030 - 2030

Published: July 10, 2024

The aim of this study was to identify with a high level confidence metabolites previously identified as predictors lameness and understand their biological relevance by carrying out pathway analyses. For the dairy cattle sector, is major challenge large impact on animal welfare farm economics. Understanding metabolic alterations during transition period associated before appearance clinical signs may allow its early detection risk prevention. annotation metabolite understanding interactions between metabolism immunity are crucial for better condition. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS) authentic standards increase in putative annotations determined predictive cows, it possible cresol, valproic acid, gluconolactone L1, L2, respectively which highest levels identification. set enrichment analysis pathways involved six significant (

Language: Английский

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Synthesis of Metabolites and Metabolite-Like Compounds Using Biocatalytic Systems

Published: Aug. 18, 2023

Methodologies for the synthesis and purification of metabolites, which have been developed following their discovery, analysis structural identification, involved in numerous life science milestones. The renewed focus on small molecule domain biological cells has also created an increasing awareness rising gap between metabolites identified prepared as pure compounds. Due to large number molecular diversity design engineering resource-efficient straightforward synthetic methodologies production attracted much interest. variety metabolic pathways provide a wonderful blueprint designing simplified routes desired metabolites. Therefore, biocatalytic systems become key enabling tools can then be utilized standards, enzyme substrates, inhibitors or products, discovery novel functions.

Language: Английский

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Synthesis of Metabolites and Metabolite-like Compounds Using Biocatalytic Systems

Metabolites, Journal Year: 2023, Volume and Issue: 13(10), P. 1097 - 1097

Published: Oct. 19, 2023

Methodologies for the synthesis and purification of metabolites, which have been developed following their discovery, analysis, structural identification, involved in numerous life science milestones. The renewed focus on small molecule domain biological cells has also created an increasing awareness rising gap between metabolites identified prepared as pure compounds. design engineering resource-efficient straightforward synthetic methodologies production diverse metabolite-like compounds attracted much interest. variety metabolic pathways provides a wonderful blueprint designing simplified routes to desired metabolites. Therefore, biocatalytic systems become key enabling tools number can then be utilized standards, enzyme substrates, inhibitors, or other products, discovery novel functions.

Language: Английский

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Lipidome isotope labelling of gut microbes (LILGM): A method of discovering endogenous microbial lipids

Talanta, Journal Year: 2024, Volume and Issue: 271, P. 125730 - 125730

Published: Feb. 1, 2024

Lipidomics analysis of gut microbiome has become critical in recent surge extensive human disease studies that investigate contributions. However, challenges remain comprehending the origins thousands lipid species produced by diverse microbes. Here, we proposed development and utilization a liquid chromatography-mass spectrometry-based approach, named lipidome isotope labelling microbes (LILGM), which enables confident detection identification endogenous microbial via 13C/15N labeling strategy high-resolution mass spectrometry. Our method leveraged vitro cultures stable isotope-labeled 13C 15 N, allowing reasonable degree incorporation into lipids over short-term inoculation. We then systematically detected spectral patterns 182 labeled our in-house data pipeline. Further bioinformatics analyses confidently identified biologically relevant from classes such as diacylglycerols (DGs), fatty acids (FAs), phosphatidylglycerols (PGs), phosphatidylethanolamines (PEs) may have profound impacts to physiology. study also demonstrated application LILGM showcasing dysregulated post antibiotic perturbation. The debiased sparse partial correlation provides insights metabolism intricacies. Overall, can provide unambiguous given biological context, instantly reflect lipidomic changes response environmental factors. believe approach potential new body knowledge combining promising analytical approaches for sensitive specific support functional studies.

Language: Английский

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