Design and Development of 3D Printing on Bioinks and Biomaterials for Implants and Tissue Engineering DOI

Murali Krishnan Ramachandran,

K. Manigandan, Rajkumar Velu

et al.

Published: Jan. 1, 2023

The capacity to produce complicated structures with high precision has made it possible make personalized implants that fit the individual anatomy of patient, improving outcomes and lowering risks. For drug testing screening, 3D-printed tissue models have been employed, current research is looking into how fabricate intricate microstructures delivery systems. This chapter explores 3D printing potential transform healthcare by making create patient-specific as well useful tissues organs for transplantation. It important consider aspects like biocompatibility, printability, mechanical qualities, degradability, cost when choosing bioinks biomaterials. In vitro crucial determine these materials' effectiveness, used in morphological, rheological, chemical, characterization materials. Finally, recent studies on biomaterials using various techniques are reviewed, including their limitations implications future research.

Language: Английский

Recent advances in defined hydrogels in organoid research DOI Creative Commons
Zhongqiao Gan,

Xinyuan Qin,

Haitao Liu

et al.

Bioactive Materials, Journal Year: 2023, Volume and Issue: 28, P. 386 - 401

Published: June 16, 2023

Organoids are in vitro model systems that mimic the complexity of organs with multicellular structures and functions, which provide great potential for biomedical tissue engineering. However, their current formation heavily relies on using complex animal-derived extracellular matrices (ECM), such as Matrigel. These often poorly defined chemical components exhibit limited tunability reproducibility. Recently, biochemical biophysical properties hydrogels can be precisely tuned, offering broader opportunities to support development maturation organoids. In this review, fundamental ECM vivo critical strategies design organoid culture summarized. Two typically derived from natural synthetic polymers applicability improve organoids presented. The representative applications incorporating into highlighted. Finally, some challenges future perspectives also discussed developing advanced technologies toward supporting research.

Language: Английский

Citations

55
Microfluidic high-throughput 3D cell culture DOI
Jihoon Ko, Dohyun Park,

Jungseub Lee

et al.

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(6), P. 453 - 469

Published: April 4, 2024

Language: Английский

Citations

22
Recent advances in Organ-on-a-Chip models: How precision engineering integrates cutting edge technologies in fabrication and characterization DOI
Sorour Sadeghzade, Mohammad Hosseini Hooshiar, Hossein Akbari

et al.

Applied Materials Today, Journal Year: 2024, Volume and Issue: 38, P. 102231 - 102231

Published: May 14, 2024

Language: Английский

Citations

9
New Drug Discovery DOI
Bancha Yingngam

Advances in medical diagnosis, treatment, and care (AMDTC) book series, Journal Year: 2023, Volume and Issue: unknown, P. 134 - 184

Published: June 16, 2023

The field of drug discovery is continually advancing with the emergence new technologies and scientific developments. Moreover, there a recent growing interest in exploiting natural products as potential source novel leads. This chapter provides an overview current state discovery, specific focus on integrative medicine. process discussed, including target identification, lead generation, optimization, preclinical clinical development, along challenges associated each step solutions. use leads explored, examples that have been transformed into drugs efforts to discover product-based drugs. Furthermore, proposes valuable insights opportunities this field, well solutions for discovering developing

Language: Английский

Citations

19
Electromembrane Extraction Provides Unprecedented Selectivity for Drugs in Cell Culture Media Used in Organoid and Organ-on-Chip Systems DOI Creative Commons
Stian Kogler, Frøydis Sved Skottvoll, Helena Hrušková

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 24, 2025

The use of organoids and organ-on-chip technologies as nonanimal methodologies in drug discovery personalized medicine is rapidly expanding. However, the complexity small volumes organoid culture samples present significant analytical challenges, e.g., analysis using liquid chromatography–mass spectrometry (LC–MS). Essentially an electrophoresis across oil membrane, electromembrane extraction (EME) offers a promising approach for measuring drugs, it is, example, compatible with such formats. Given potential technology, there need to assess purity EME extracts ensure EME's compatibility high-throughput, downstream analysis. This study evaluates effectiveness sample cleanup various common cell media used organs-on-chips. were spiked 90 small-molecule drugs. Using gel (sodium dodecyl sulfate polyacrylamide electrophoresis), high-resolution nuclear magnetic resonance spectroscopy, LC–MS, we demonstrate that provides exhaustive removal unwanted medium components (proteins, polar molecules, apolar/neutral molecules) while selectively extracting was demonstrated human stem-cell-derived liver organoids, allowing simple detection monitoring telltale cytochrome P450 metabolism. Taken together, our observations highlight unprecedented ability provide matrixes technology.

Language: Английский

Citations

0
Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin DOI Creative Commons
Helena Hrušková, Christine Olsen, Roman Řemínek

et al.

Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1717, P. 464669 - 464669

Published: Jan. 20, 2024

Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal-free studies development, disease, and drug discovery. The medium organoid models Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed liquid chromatography-mass spectrometry (LC-MS). However, complexity is a major challenge, as matrix interferences reduce sensitivity selectivity, even optimized LC-MS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the background signal, allowing reduced affecting analysis human insulin.

Language: Английский

Citations

3
An FDA-Validated, Self-Cleaning Liquid Chromatography–Mass Spectrometry System for Determining Small-Molecule Drugs and Metabolites in Organoid/Organ-on-Chip Medium DOI Creative Commons
Stian Kogler,

Gustav Mathingsdal Pedersen,

Felipe Martínez-Ramírez

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(29), P. 12129 - 12138

Published: July 10, 2024

As organoids and organ-on-chip (OoC) systems move toward preclinical clinical applications, there is an increased need for method validation. Using a liquid chromatography-mass spectrometry (LC-MS)-based approach, we developed measuring small-molecule drugs metabolites in the cell medium directly sampled from liver organoids/OoC systems. The LC-MS setup was coupled to automatic filtration filter flush system with online solid-phase extraction (SPE), allowing robust automated sample cleanup/analysis. For matrix, rich in, e.g., protein, salts, amino acids, no preinjection preparation steps (protein precipitation, SPE, etc.) were necessary. approach demonstrated tolbutamide its metabolite, 4-hydroxytolbutamide (4HT). validated analysis of media human stem cell-derived cultured static conditions on microfluidic platform according Food Drug Administration (FDA) guidelines regards selectivity, matrix effects, accuracy, precision, etc. allows hundreds injections without replacing chromatography hardware. In summary, drug/metabolite organoids/OoCs can be performed robustly minimal preparation.

Language: Английский

Citations

3
Trends in organ-on-a-chip for pharmacological analysis DOI
Xinmei Xu,

S. Cheung,

Xiaomeng Jia

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 180, P. 117905 - 117905

Published: Aug. 9, 2024

Language: Английский

Citations

3
Technological advances for analyzing the content of organ-on-a-chip by mass spectrometry DOI Creative Commons
Darya Hadavi,

Ilona Tosheva,

Tiffany Porta Siegel

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: May 22, 2023

Three-dimensional (3D) cell cultures, including organ-on-a-chip (OOC) devices, offer the possibility to mimic human physiology conditions better than 2D models. The devices have a wide range of applications, mechanical studies, functional validation, and toxicology investigations. Despite many advances in this field, major challenge with use organ-on-a-chips relies on lack online analysis methods preventing real-time observation cultured cells. Mass spectrometry is promising analytical technique for excretes from This due its high sensitivity, selectivity, ability tentatively identify large variety unknown compounds, ranging metabolites, lipids, peptides proteins. However, hyphenation MS largely hampered by nature media used, presence nonvolatile buffers. turn stalls straightforward connection outlet MS. To overcome challenge, multiple been made pre-treat samples right after just before In review, we summarised these technological exhaustively evaluated their benefits shortcomings successful

Language: Английский

Citations

8
Simultaneous LC–MS determination of glucose regulatory peptides secreted by stem cell–derived islet organoids DOI Creative Commons
Christine Olsen, Chencheng Wang, Aleksandra Aizenshtadt

et al.

Electrophoresis, Journal Year: 2023, Volume and Issue: 44(21-22), P. 1682 - 1697

Published: Aug. 13, 2023

For studying stem cell-derived islet organoids (SC-islets) in an organ-on-chip (OoC) platform, we have developed a reversed-phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) method allowing for simultaneous determination of insulin, somatostatin-14, and glucagon, with improved matrix robustness compared to earlier methodology. Combining phenyl/hexyl-C18 separations using 2.1 mm inner diameter LC columns triple quadrupole spectrometry, identification quantification were secured negligible variance retention time quantifier/qualifier ratios, levels carryover (<2%), sufficient precision (±10% RSD) accuracy (±15% relative error) without use internal standard. The obtained lower limits 0.2 µg/L human 0.1 0.05 glucagon. here-developed RPLC-MS/MS showed that the SC-islets insulin response dependent on glucose concentration, produce release somatostatin-14 these peptide hormones was compatible unfiltered offline sample collection from cultivated pumpless, recirculating OoC (rOoC) platform. background secretion not significantly different rOoC device standard cell culture well-plate. Taken together, is well suited multi-hormone measurements

Language: Английский

Citations

7