Retention mechanisms of amitriptyline and its impurities on amide, amino, diol, and silica columns in hydrophilic interaction liquid chromatography

Journal of Separation Science, Journal Year: 2024, Volume and Issue: 47(9-10)

Published: May 1, 2024

Hydrophilic interaction liquid chromatography (HILIC) has been widely applied to challenging analysis in biomedical and pharmaceutical fields, bridging the gap between normal-phase high-performance reversed-phase (RP-HPLC). This paper comprehensively explores retention mechanisms of amitriptyline its impurities A, B, C, D, F, G on amide, amino, diol, silica columns. Dual HILIC/RP-HPLC were developed, transitional points HILIC RP-HPLC calculated Adsorption partition contributions overall evaluated using Python software regions. The cation exchange mechanism dominates for ionized analytes column (R

Language: Английский

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Simplified Synthesis of Poly(ethyleneimine)-Modified Silica Particles and Their Application in Oligosaccharide Isolation Methods

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9465 - 9465

Published: Aug. 30, 2024

There are great challenges in the field of natural product isolation and purification pharmacological study oligosaccharide monomers. And these processes still universal problems products (NPs), traditional Chinese medicine (TCM), omics, etc. The same polymer-modified materials designed for special separation oligosaccharides, named Sil-epoxy-PEI Sil-chloropropyl-PEI, were synthesized via two different methods characterized by scanning electron microscopy combined with energy spectrum analysis, Fourier transform infrared spectroscopy, thermogravimetric zeta potential as well surface area Several nucleotide/nucleoside molecules polarities selectivities successfully isolated our laboratory using stainless-steel columns filled material. In addition, saccharide probes oligosaccharides mixtures water extracts Morinda officinalis compared HILIC mode. results showed that resolution separations representative analytes column was higher than Sil-chloropropyl-PEI column, developed stationary phase exhibited improved performance to hydrothermal carbon, amide other previously reported.

Language: Английский

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Field-Assisted liquid chromatography for enhanced separation and Purification: A comprehensive review

Separation and Purification Technology, Journal Year: 2024, Volume and Issue: 354, P. 128858 - 128858

Published: July 18, 2024

Language: Английский

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HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements

Separations, Journal Year: 2024, Volume and Issue: 11(2), P. 45 - 45

Published: Jan. 30, 2024

This article highlights the fundamental aspects of hydrophilic interaction liquid chromatography (HILIC) on poly-hydroxyl stationary phases to analyze non-derivatized mono- and disaccharides, including commonly consumed carbohydrates like glucose, fructose, sucrose, lactose. The evaporative light-scattering detector (ELSD) is utilized as an alternative MS detector, separation system’s selectivity allows anomers monosaccharides. study also includes a rapid method for determining isomaltulose (Palatinose), which was validated applied food supplement samples available in Czech market, even those with high protein content. Additionally, separated from sucrose just 13 min.

Language: Английский

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Rational Development of Functional Hydrophilic Polymer for the Characterization of Protein Glycosylation in Bovine Milk and Colostrum

Published: Jan. 1, 2024

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Language: Английский

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Rational development of functional hydrophilic polymer to characterize site-specific glycan differences between bovine milk and colostrum

Food Chemistry, Journal Year: 2024, Volume and Issue: 460, P. 140669 - 140669

Published: July 27, 2024

Language: Английский

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Chromatographic evaluation and application of nitrogenous heterocyclic ring-bonded stationary phase in hydrophilic interaction liquid chromatography

Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1734, P. 465315 - 465315

Published: Aug. 27, 2024

Language: Английский

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Hydrophilic interaction liquid chromatography: An efficient tool for assessing thorium interaction with phosphorylated biomimetic peptides

Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1735, P. 465341 - 465341

Published: Sept. 3, 2024

Language: Английский

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Expression and immobilization of novel N-glycan-binding protein for highly efficient purification and enrichment of N-glycans, N-glycopeptides, and N-glycoproteins

Analytical and Bioanalytical Chemistry, Journal Year: 2024, Volume and Issue: 416(29), P. 6859 - 6868

Published: Oct. 16, 2024

Language: Английский

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Comprehensive analysis of the effective and intra-particle diffusion of weakly retained compounds in silica hydrophilic interaction liquid chromatography columns

Journal of Chromatography A, Journal Year: 2023, Volume and Issue: 1713, P. 464529 - 464529

Published: Nov. 23, 2023

Language: Английский

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