Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 26, 2024
pH
and
peroxynitrite
(ONOO–)
are
two
critical
biomarkers
to
unveil
the
corresponding
status
of
endoplasmic
reticulum
(ER)
stress
mitochondrial
dysfunction,
which
closely
related
Alzheimer's
disease
(AD).
Simultaneously
monitoring
ONOO–
fluctuations
in
ER
mitochondria
during
AD
progression
is
pivotal
for
clarifying
interplay
between
disorders
organelles
revealing
pathogenesis.
Herein,
we
designed
synthesized
a
dual-channel
fluorescent
probe
(DCFP)
visualize
mitochondria.
DCFP
possessed
excellent
sensitivity
selectivity
without
spectral
crosstalk
was
utilized
analytes
within
model
cells
larval
zebrafish.
Importantly,
could
preferentially
target
normal
be
enriched
after
depolarization.
With
aid
DCFP,
slower
acidification
rate
than
that
induced
by
Aβ
oligomers
(AβOs)
first
identified,
ascribed
relief
AβOs-triggered
through
Ca2+
migration
from
Moreover,
continuous
exposure
AβOs
led
overload,
accelerating
overproduction
As
result,
intracellular
oxidative
levels
were
elevated,
further
exacerbating
aggravating
turn.
The
advanced
understanding
potential
this
work
may
offer
new
insights
methodologies
studying
developed
also
employed
study
other
diseases
dysfunction.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(5), P. 2041 - 2051
Published: Jan. 25, 2024
Ferroptosis
is
critical
in
the
treatment
of
tumor
therapies.
Thus,
monitoring
reactive
oxygen
species
(ROS)
great
significance
for
accurate
assessment
ferroptosis
without
any
interference.
However,
current
probes
ROS
during
suffer
from
a
drawback
that
consume
detection,
which
inhibits
process
and
thus
affects
accuracy
effectiveness
ferroptosis.
Herein,
new
fluorescent
donor
probe,
TFMU-SO2D,
with
combination
moiety
SO2
designed
synthesized
by
introducing
aryl
boronate
moieties
could
give
it
ability
to
effectively
recognize
ONOO–.
The
released
excess
glutathione
regulate
oxidative
stress
elevating
levels,
would
offset
depletion
TFMU-SO2D
ensure
process.
experimental
results
demonstrated
possessed
satisfactory
performance
ONOO–
as
well
simultaneously
releasing
stimulated
monensin
erastin
RSL3.
Additionally,
capability
synergized
inhibit
viability
cancer
cells
was
CCK8
assay,
may
be
due
fact
can
potentiate
cell
death
increasing
level.
Overall,
these
combined
indicated
possesses
excellent
precisely
monitor
interference,
significant
accurately
accessing
ferroptosis,
treatment,
drug
development.
Analytical Sciences,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 31, 2025
The
physiological
and
pathological
processes
in
lungs
indicated
that
ONOO-
acted
as
an
informative
indicator
for
pulmonary
functioning
status
with
the
corresponding
dynamics,
especially
permissive
hypercapnia.
Herein,
peroxynitrite
(ONOO-)
detection
imaging
living
lung
cells,
imidazo-pyridin
derived
fluorescent
probe
IDPD-ONOO
was
developed.
recognized
to
exhibit
a
fluorescence
response
at
490
nm
excitation
wavelength
355
nm.
advantages
of
developed
included
practical
linear
range
(0-100
μM),
high
sensitivity
(Limit
Detection
0.005
rapid
(within
15
min),
pH
tolerance
(7.0-9.0),
selectivity,
low
cyto-toxicity.
Furthermore,
achieved
intracellular
both
exogenous
endogenous
level,
which
meaningful
research
on
mechanism
optimized
optical
implements.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 14, 2025
Embryonic
development
is
the
beginning
of
life,
and
various
kinds
bioactive
molecules
are
implicated
in
this
crucial
process.
Especially
its
early
stage,
some
important
biochemical
reactions
regulating
physiological
balance
may
be
resuscitated.
Thus,
revealing
dynamic
changes
during
embryonic
to
elucidation
biological
phenomena.
Peroxynitrite
(ONOO-)
a
typical
signaling
molecule
intercellular
communication.
However,
up
now,
no
work
has
studied
fluctuation
ONOO-
due
low
content,
especially
mammals.
Herein,
polymeric
nanobeacon
that
integrates
an
ONOO--responsive
degradable
scaffold
fluorescence
amplification
module,
named
IFN,
was
designed
selectively
sense
with
high
sensitivity.
By
virtue
specific
dye,
sensitively
detected
range
0-4.5
μM
detection
limit
20.4
nM.
From
attractive
results,
sudden
increase
content
after
fertilization
observed
mammalian
model,
while
level
decreased
slightly
at
four-cell
eight-cell
stages,
finally
reaching
equilibrium
throughout
morula
blastocyst
stages.
This
phenomenon
resuscitation
ovotids,
activation
life
events
by
fertilization,
subsequent
establishment
balance.
not
only
suggests
plays
positive
role
normal
but
also
highlights
molecular
occurring
initial
phase
life.
Furthermore
it
opens
new
avenues
for
monitoring
chemical
development.
